DOI: 10.1126/science.2047860. Monolayer Crystallization of Flagellar L-P Rings by Sequential Addition and Depletion of Lipid

Monolayer Crystallization of Flagellar L-P Rings by Sequential Addition and Depletion of Lipid

10.1126/science.2047860

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The L-P ring complex is thought to be a molecular bushing that supports flagellar motor rotation at about 10,000 revolutions per minute with presumably very little friction. Structural studies of this complex have been limited because only very small amount of samples are available. Therefore devising an efficient method of crystallization was essential. The addition of a phospholipid and its subsequent slow depletion by phospholipase A 2 have been used to successfully grow well-ordered monolayer crystals that extend up to about 10 micrometers. The interaction of the L-P ring complex with lipid membranes was also visualized during this process.

Bibliography

Akiba, T., Yoshimura, H., & Namba, K. (1991). Monolayer Crystallization of Flagellar L-P Rings by Sequential Addition and Depletion of Lipid. Science, 252(5012), 1544â1546.

Authors 3

Toshihiko Akiba (first)
Hideyuki Yoshimura (additional)
Keiichi Namba (additional)

References 11 Referenced 52

AIZAWA, S, PURIFICATION AND CHARACTERIZATION OF THE FLAGELLAR HOOK BASAL BODY COMPLEX OF SALMONELLA-TYPHIMURIUM, JOURNAL OF BACTERIOLOGY 161: 836 (1985). (10.1128/jb.161.3.836-849.1985) / JOURNAL OF BACTERIOLOGY (1985)
DEPAMPHILIS, M.L., FINE STRUCTURE AND ISOLATION OF HOOK-BASAL BODY COMPLEX OF FLAGELLA FROM ESCHERICHIA-COLI AND BACILLUS-SUBTILIS, JOURNAL OF BACTERIOLOGY 105: 384 (1971). (10.1128/jb.105.1.384-395.1971) / JOURNAL OF BACTERIOLOGY (1971)
JONES, C.J., IDENTIFICATION OF PROTEINS OF THE OUTER (L AND P) RINGS OF THE FLAGELLAR BASAL BODY OF ESCHERICHIA-COLI, JOURNAL OF BACTERIOLOGY 169: 1489 (1987). (10.1128/jb.169.4.1489-1492.1987) / JOURNAL OF BACTERIOLOGY (1987)
JONES, C.J., L-RING, P-RING, AND M-RING PROTEINS OF THE FLAGELLAR BASAL BODY OF SALMONELLA-TYPHIMURIUM - GENE-SEQUENCES AND DEDUCED PROTEIN SEQUENCES, JOURNAL OF BACTERIOLOGY 171: 3890 (1989). (10.1128/jb.171.7.3890-3900.1989) / JOURNAL OF BACTERIOLOGY (1989)
JONES, C.J., STOICHIOMETRIC ANALYSIS OF THE FLAGELLAR HOOK (BASAL-BODY) COMPLEX OF SALMONELLA-TYPHIMURIUM, JOURNAL OF MOLECULAR BIOLOGY 212: 377 (1990). (10.1016/0022-2836(90)90132-6) / JOURNAL OF MOLECULAR BIOLOGY (1990)
KLEFFEL, B, SECONDARY STRUCTURE OF A CHANNEL-FORMING PROTEIN - PORIN FROM ESCHERICHIA-COLI OUTER MEMBRANES, EMBO JOURNAL 4: 1589 (1985). (10.1002/j.1460-2075.1985.tb03821.x) / EMBO JOURNAL (1985)
10.1126/science.6322311
Nikaido, H., Escherichia coli and Salmonela typhimurium: 7 (1987). / Escherichia coli and Salmonela typhimurium (1987)
PATTERSONDELAFI.J, NEW FLA GENE IN SALMONELLA-TYPHIMURIUM - FLAR - AND ITS MUTANT PHENOTYPE-SUPERHOOKS, ARCHIV FUR MIKROBIOLOGIE 90: 107 (1973). (10.1007/BF00414513) / ARCHIV FUR MIKROBIOLOGIE (1973)
STALLMEYER, MJB, IMAGE-RECONSTRUCTION OF THE FLAGELLAR BASAL BODY OF SALMONELLA-TYPHIMURIUM, JOURNAL OF MOLECULAR BIOLOGY 205: 519 (1989). (10.1016/0022-2836(89)90223-4) / JOURNAL OF MOLECULAR BIOLOGY (1989)
YOSHIMURA, H, 2-DIMENSIONAL CRYSTALLIZATION OF PROTEINS ON MERCURY, ULTRAMICROSCOPY 32: 265 (1990). (10.1016/0304-3991(90)90004-6) / ULTRAMICROSCOPY (1990)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 10:11 p.m.)
Indexed	1 day, 7 hours ago (Aug. 22, 2025, 12:47 a.m.)
Issued	34 years, 2 months ago (June 14, 1991)
Published	34 years, 2 months ago (June 14, 1991)
Published Print	34 years, 2 months ago (June 14, 1991)

Funders 0

None

BibTeX

@article{Akiba_1991, title={Monolayer Crystallization of Flagellar L-P Rings by Sequential Addition and Depletion of Lipid}, volume={252}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2047860}, DOI={10.1126/science.2047860}, number={5012}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Akiba, Toshihiko and Yoshimura, Hideyuki and Namba, Keiichi}, year={1991}, month=jun, pages={1544–1546} }

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