DOI: 10.1126/science.2024121. Three-Dimensional Readout of Flash X-Ray Images of Living Sperm in Water by Atomic-Force Microscopy

Three-Dimensional Readout of Flash X-Ray Images of Living Sperm in Water by Atomic-Force Microscopy

10.1126/science.2024121

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The imaging of living specimens in water by x-ray microscopy can be greatly enhanced with the use of an intense flash x-ray source and sophisticated technologies for reading x-ray images. A subnanosecond x-ray pulse from a laser-produced plasma was used to record the x-ray image of living sea urchin sperm in an x-ray resist. The resist relief was visualized at high resolution by atomic-force microscopy. Internal structure of the sperm head was evident, and the carbon density in a flagellum was estimated from the relief height.

Bibliography

Tomie, T., Shimizu, H., Majima, T., Yamada, M., Kanayama, T., Kondo, H., Yano, M., & Ono, M. (1991). Three-Dimensional Readout of Flash X-Ray Images of Living Sperm in Water by Atomic-Force Microscopy. Science, 252(5006), 691â693.

Authors 8

T. Tomie (first)
H. Shimizu (additional)
T. Majima (additional)
M. Yamada (additional)
T. Kanayama (additional)
H. Kondo (additional)
M. Yano (additional)
M. Ono (additional)

References 14 Referenced 69

Cotton R. A. paper presented at the International Conference on X-Ray Microscopy King's College London September (1990).
ALLEN, R.D., A REINVESTIGATION OF CROSS-SECTIONS OF CILIA, JOURNAL OF CELL BIOLOGY 37: 825 (1968). (10.1083/jcb.37.3.825) / JOURNAL OF CELL BIOLOGY (1968)
BINNIG, G, ATOMIC FORCE MICROSCOPE, PHYSICAL REVIEW LETTERS 56: 930 (1986). (10.1103/PhysRevLett.56.930) / PHYSICAL REVIEW LETTERS (1986)
Cheng, P. C., X-ray Microscopy: 65 (1987). / X-ray Microscopy (1987)
Eason R. W. Annual Report. Rutherford Appleton Laboratory (1985).
10.1126/science.406670
10.1126/science.3964958
Giudice, G., The Sea Urchin Embryo: 23 (1987). / The Sea Urchin Embryo (1987)
Jacobsen, C., X-ray Microscopy 2: 253 (1988). / X-ray Microscopy (1988)
Kirz, J., X-ray Microscopy: 262 (1984). / X-ray Microscopy (1984)
LONDON, R.A., WAVELENGTH CHOICE FOR SOFT-X-RAY LASER HOLOGRAPHY OF BIOLOGICAL SAMPLES, APPLIED OPTICS 28: 3397 (1989). (10.1364/AO.28.003397) / APPLIED OPTICS (1989)
ROSSER, R.J., NONDESTRUCTIVE SINGLE-SHOT SOFT-X-RAY LITHOGRAPHY AND CONTACT MICROSCOPY USING A LASER-PRODUCED PLASMA SOURCE, APPLIED OPTICS 26: 4313 (1987). (10.1364/AO.26.004313) / APPLIED OPTICS (1987)
Sayre D. X-ray Microscopy 2 (1988). (10.1007/978-3-540-39246-0)
SOLEM, J.C., IMAGING BIOLOGICAL SPECIMENS WITH HIGH-INTENSITY SOFT X-RAYS, JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS 3: 1551 (1986). (10.1364/JOSAB.3.001551) / JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS (1986)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 9:26 p.m.)
Indexed	1 year ago (Aug. 1, 2024, 10:51 p.m.)
Issued	34 years, 3 months ago (May 3, 1991)
Published	34 years, 3 months ago (May 3, 1991)
Published Print	34 years, 3 months ago (May 3, 1991)

Funders 0

None

BibTeX

@article{Tomie_1991, title={Three-Dimensional Readout of Flash X-Ray Images of Living Sperm in Water by Atomic-Force Microscopy}, volume={252}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2024121}, DOI={10.1126/science.2024121}, number={5006}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Tomie, T. and Shimizu, H. and Majima, T. and Yamada, M. and Kanayama, T. and Kondo, H. and Yano, M. and Ono, M.}, year={1991}, month=may, pages={691–693} }

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