DOI: 10.1126/science.1990439. Determination of Membrane Protein Structure by Rotational Resonance NMR: Bacteriorhodopsin

Determination of Membrane Protein Structure by Rotational Resonance NMR: Bacteriorhodopsin

10.1126/science.1990439

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Rotationally resonant magnetization exchange, a new nuclear magnetic resonance (NMR) technique for measuring internuclear distances between like spins in solids, was used to determine the distance between the C-8 and C-18 carbons of retinal in two model compounds and in the membrane protein bacteriorhodopsin. Magnetization transfer between inequivalent spins with an isotropic shift separation, δ, is driven by magic angle spinning at a speed ω r that matches the rotational resonance condition δ = n ω r , where n is a small integer. The distances measured in this way for both the 6-s- cis - and 6-s- trans -retinoic acid model compounds agreed well with crystallographically known distances. In bacteriorhodopsin the exchange trajectory between C-8 and C-18 was in good agreement with the internuclear distance for a 6-s- trans configuration [4.2 angstroms (Å)] and inconsistent with that for a 6-s- cis configuration (3.1 Å). The results illustrate that rotational resonance can be used for structural studies in membrane proteins and in other situations where diffraction and solution NMR techniques yield limited information.

Bibliography

Creuzet, F., McDermott, A., Gebhard, R., van der Hoef, K., Spijker-Assink, M. B., Herzfeld, J., Lugtenburg, J., Levitt, M. H., & Griffin, R. G. (1991). Determination of Membrane Protein Structure by Rotational Resonance NMR: Bacteriorhodopsin. Science, 251(4995), 783â786.

Authors 9

F. Creuzet (first)
A. McDermott (additional)
R. Gebhard (additional)
K. van der Hoef (additional)
M. B. Spijker-Assink (additional)
J. Herzfeld (additional)
J. Lugtenburg (additional)
M. H. Levitt (additional)
R. G. Griffin (additional)

References 31 Referenced 215

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Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 10:04 p.m.)
Indexed	2 months, 1 week ago (June 26, 2025, 9:29 a.m.)
Issued	34 years, 6 months ago (Feb. 15, 1991)
Published	34 years, 6 months ago (Feb. 15, 1991)
Published Print	34 years, 6 months ago (Feb. 15, 1991)

Funders 0

None

BibTeX

@article{Creuzet_1991, title={Determination of Membrane Protein Structure by Rotational Resonance NMR: Bacteriorhodopsin}, volume={251}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1990439}, DOI={10.1126/science.1990439}, number={4995}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Creuzet, F. and McDermott, A. and Gebhard, R. and van der Hoef, K. and Spijker-Assink, M. B. and Herzfeld, J. and Lugtenburg, J. and Levitt, M. H. and Griffin, R. G.}, year={1991}, month=feb, pages={783–786} }

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  "abstract": "<jats:p>\n            Rotationally resonant magnetization exchange, a new nuclear magnetic resonance (NMR) technique for measuring internuclear distances between like spins in solids, was used to determine the distance between the C-8 and C-18 carbons of retinal in two model compounds and in the membrane protein bacteriorhodopsin. Magnetization transfer between inequivalent spins with an isotropic shift separation, \u03b4, is driven by magic angle spinning at a speed \u03c9\n            <jats:sub>r</jats:sub>\n            that matches the rotational resonance condition \u03b4 =\n            <jats:italic>n</jats:italic>\n            \u03c9\n            <jats:sub>r</jats:sub>\n            , where\n            <jats:italic>n</jats:italic>\n            is a small integer. The distances measured in this way for both the 6-s-\n            <jats:italic>cis</jats:italic>\n            - and 6-s-\n            <jats:italic>trans</jats:italic>\n            -retinoic acid model compounds agreed well with crystallographically known distances. In bacteriorhodopsin the exchange trajectory between C-8 and C-18 was in good agreement with the internuclear distance for a 6-s-\n            <jats:italic>trans</jats:italic>\n            configuration [4.2 angstroms (\u00c5)] and inconsistent with that for a 6-s-\n            <jats:italic>cis</jats:italic>\n            configuration (3.1 \u00c5). The results illustrate that rotational resonance can be used for structural studies in membrane proteins and in other situations where diffraction and solution NMR techniques yield limited information.\n          </jats:p>",
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