DOI: 10.1126/science.193.4249.227. Intensity Dependence of the Fluorescence Lifetime of in vivo Chlorophyll Excited by a Picosecond Light Pulse

Intensity Dependence of the Fluorescence Lifetime of in vivo Chlorophyll Excited by a Picosecond Light Pulse

10.1126/science.193.4249.227

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

New data on intensity-dependent lifetimes indicate that all previous in vivo fluorescence studies of chlorophyll by picosecond techniques must be reinterpreted. Anomalously short lifetimes result from high-intensity effects due to exciton-exciton annihilation processes. Measurements in Chlorella pyrenoidosa with single-pulse, low-intensity excitation indicate a longer "true" lifetime of 650 ± 150 picoseconds.

Bibliography

Campillo, A. J., Kollman, V. H., & Shapiro, S. L. (1976). Intensity Dependence of the Fluorescence Lifetime of in vivo Chlorophyll Excited by a Picosecond Light Pulse. Science, 193(4249), 227â229.

Authors 3

A. J. Campillo (first)
V. H. Kollman (additional)
S. L. Shapiro (additional)

References 27 Referenced 63

BARBER J COMMUNICATION.
BEDDARD, G.S., FLUORESCENCE LIFETIMES IN PHOTOSYNTHETIC UNIT, NATURE 258: 166 (1975). (10.1038/258166a0) / NATURE (1975)
BORISOV, A.Y., JOURNAL OF BIOENERGETICS 3: 211 (1972). (10.1007/BF01515969) / JOURNAL OF BIOENERGETICS (1972)
BRETON, J, PRESENTATION OF TRIPLET STATE OF CHLOROPHYLL IN CHLOROPLASTIC LAMELLAE, COMPTES RENDUS HEBDOMADAIRES DES SEANCES DE L ACADEMIE DES SCIENCES SERIE D 271: 1094 (1970). / COMPTES RENDUS HEBDOMADAIRES DES SEANCES DE L ACADEMIE DES SCIENCES SERIE D (1970)
CAMPILLO, A.J., PICOSECOND EXCITON ANNIHILATION IN PHOTOSYNTHETIC SYSTEMS, BIOPHYSICAL JOURNAL 16: 93 (1976). (10.1016/S0006-3495(76)85666-4) / BIOPHYSICAL JOURNAL (1976)
Campillo A. J. in preparation.
CAMPILLO A.J. UNPUBLISHED RESULTS.
Goad W. B. in preparation.
HYER, R.C., LASER-INITIATED KRYTRON-SWITCHED BLUMLEIN STRUCTURE FOR PULSE SELECTION, REVIEW OF SCIENTIFIC INSTRUMENTS 46: 1333 (1975). (10.1063/1.1134066) / REVIEW OF SCIENTIFIC INSTRUMENTS (1975)
KOLLMAN, V.H., PHOTOSYNTHETIC STUDIES WITH A 10-PSEC RESOLUTION STREAK CAMERA, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 63: 917 (1975). (10.1016/0006-291X(75)90656-7) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1975)
Latimer, P., Research in Photosynthesis: 107 (1957). / Research in Photosynthesis (1957)
10.1126/science.124.3222.585
MAUZERALL, D, MULTIPLE EXCITATIONS IN PHOTOSYNTHETIC SYSTEMS, BIOPHYSICAL JOURNAL 16: 87 (1976). (10.1016/S0006-3495(76)85665-2) / BIOPHYSICAL JOURNAL (1976)
10.1126/science.164.3877.301
MULLER, A, LIGHT-INTENSITY DEPENDENCE OF IN VIVO FLUORESCENCE LIFETIME OF CHLOROPHYLL, PHOTOCHEMISTRY AND PHOTOBIOLOGY 9: 113 (1969). (10.1111/j.1751-1097.1969.tb05916.x) / PHOTOCHEMISTRY AND PHOTOBIOLOGY (1969)
Murty, N. R., Photochemistry and Photobiology 4: 917 (1965). (10.1111/j.1751-1097.1965.tb07940.x) / Photochemistry and Photobiology (1965)
NICHOLSON, W.J., MEASUREMENT OF FLUORESCENT LIFETIMES OF CHLORELLA AND PORPHYRIDIUM IN WEAK LIGHT, BIOCHIMICA ET BIOPHYSICA ACTA 143: 577 (1967). (10.1016/0005-2728(67)90063-1) / BIOCHIMICA ET BIOPHYSICA ACTA (1967)
PASCHENKO, V.Z., PROBING KINETICS OF PHOTOSYSTEM-I AND PHOTOSYSTEM-II FLUORESCENCE IN PEA CHLOROPLASTS ON A PICOSECOND PULSE FLUOROMETER, BIOCHIMICA ET BIOPHYSICA ACTA 408: 143 (1975). (10.1016/0005-2728(75)90006-7) / BIOCHIMICA ET BIOPHYSICA ACTA (1975)
PASCHENKO, V.Z., KVANTOVAYA ELECTRONI 2: 1336 (1975). / KVANTOVAYA ELECTRONI (1975)
RAHMAN, T. S., PHYSICA STATUS SOLIDI B-BASIC RESEARCH 58: 715 (1973). (10.1002/pssb.2220580233) / PHYSICA STATUS SOLIDI B-BASIC RESEARCH (1973)
SEIBERT, M, PICOSECOND FLUORESCENT KINETICS OF IN-VIVO CHLOROPHYLL, BIOCHIMICA ET BIOPHYSICA ACTA 292: 493 (1973). (10.1016/0005-2728(73)90054-6) / BIOCHIMICA ET BIOPHYSICA ACTA (1973)
SEIBERT, M, PROBING PHOTOSYNTHESIS ON A PICOSECOND TIME SCALE - EVIDENCE FOR PHOTOSYSTEM-I AND PHOTOSYSTEM-II FLUORESCENCE IN CHLOROPLASTS, BIOPHYSICAL JOURNAL 14: 269 (1974). (10.1016/S0006-3495(74)85915-1) / BIOPHYSICAL JOURNAL (1974)
SHAPIRO, S.L., ENERGY-TRANSFER IN PHOTOSYNTHESIS - PIGMENT CONCENTRATION EFFECTS AND FLUORESCENT LIFETIMES, FEBS LETTERS 54: 358 (1975). (10.1016/0014-5793(75)80939-2) / FEBS LETTERS (1975)
SINGHAL, G.S., MEASUREMENT OF FLUORESCENCE LIFETIME OF CHLOROPHYLL A IN VIVO, BIOPHYSICAL JOURNAL 9: 586 (1969). (10.1016/S0006-3495(69)86405-2) / BIOPHYSICAL JOURNAL (1969)
SWENBERG C.E. COMMUNICATION.
Swenberg C. in preparation.
YU, W, FLUORESCENT KINETICS OF CHLOROPHYLL IN PHOTOSYSTEMS-I AND PHOTOSYSTEMS-II ENRICHED FRACTIONS OF SPINACH, BIOCHIMICA ET BIOPHYSICA ACTA 387: 159 (1975). (10.1016/0005-2728(75)90060-2) / BIOCHIMICA ET BIOPHYSICA ACTA (1975)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 11:51 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 4:34 p.m.)
Indexed	1 year, 7 months ago (Jan. 11, 2024, 7:04 p.m.)
Issued	49 years, 1 month ago (July 16, 1976)
Published	49 years, 1 month ago (July 16, 1976)
Published Print	49 years, 1 month ago (July 16, 1976)

Funders 0

None

BibTeX

@article{Campillo_1976, title={Intensity Dependence of the Fluorescence Lifetime of in vivo Chlorophyll Excited by a Picosecond Light Pulse}, volume={193}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.193.4249.227}, DOI={10.1126/science.193.4249.227}, number={4249}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Campillo, A. J. and Kollman, V. H. and Shapiro, S. L.}, year={1976}, month=jul, pages={227–229} }

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