DOI: 10.1126/science.1910206. Atomic Force Microscopy and Dissection of Gap Junctions

Atomic Force Microscopy and Dissection of Gap Junctions

10.1126/science.1910206

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

An atomic force microscope (AFM) was used to study the structure of isolated hepatic gap junctions in phosphate-buffered saline (PBS). The thickness of these gap junctions appears to be 14.4 nanometers, close to the dimensions reported by electron microscopy (EM). When an increasing force is applied to the microscope tip, the top membrane of the gap junction can be "dissected" away, leaving the extracellular domains of the bottom membrane exposed. When such "force dissection" is performed on samples both trypsinized and fixed with glutaraldehyde, the hexagonal array of gap junction hemichannels is revealed, with a center-to-center spacing of 9.1 nanometers.

Bibliography

Hoh, J. H., Lal, R., John, S. A., Revel, J.-P., & Arnsdorf, M. F. (1991). Atomic Force Microscopy and Dissection of Gap Junctions. Science, 253(5026), 1405â1408.

Authors 5

Jan H. Hoh (first)
Ratneshwar Lal (additional)
Scott A. John (additional)
Jean-Paul Revel (additional)
Morton F. Arnsdorf (additional)

References 24 Referenced 189

BERNARDINI, G, GAP JUNCTION CRYSTALLIZATION IN LENS FIBERS AFTER AN INCREASE IN CELL CALCIUM, INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 21: 291 (1981). / INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (1981)
BINNIG, G, ATOMIC FORCE MICROSCOPE, PHYSICAL REVIEW LETTERS 56: 930 (1986). (10.1103/PhysRevLett.56.930) / PHYSICAL REVIEW LETTERS (1986)
BUTT, H.J., IMAGING THE MEMBRANE-PROTEIN BACTERIORHODOPSIN WITH THE ATOMIC FORCE MICROSCOPE, BIOPHYSICAL JOURNAL 58: 1473 (1990). (10.1016/S0006-3495(90)82492-9) / BIOPHYSICAL JOURNAL (1990)
DEWEY, M.M., INTERCELLULAR CONNECTION BETWEEN SMOOTH MUSCLE CELLS - NEXUS, SCIENCE 137: 670 (1962). (10.1126/science.137.3531.670.b) / SCIENCE (1962)
10.1126/science.2928794
EDSTROM, R.D., DIRECT VISUALIZATION OF PHOSPHORYLASE-PHOSPHORYLASE KINASE COMPLEXES BY SCANNING TUNNELING AND ATOMIC FORCE MICROSCOPY, BIOPHYSICAL JOURNAL 58: 1437 (1990). (10.1016/S0006-3495(90)82489-9) / BIOPHYSICAL JOURNAL (1990)
FINBOW, M, INDEPENDENT LINES OF EVIDENCE SUGGESTING A MAJOR GAP JUNCTIONAL PROTEIN WITH A MOLECULAR-WEIGHT OF 26,000, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 77: 970 (1980). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1980)
10.1126/science.377490
GOODENOUGH, D.A., TOPOLOGICAL DISTRIBUTION OF 2 CONNEXIN32 ANTIGENIC SITES IN INTACT AND SPLIT RODENT HEPATOCYTE GAP-JUNCTIONS, JOURNAL OF CELL BIOLOGY 107: 1817 (1988). (10.1083/jcb.107.5.1817) / JOURNAL OF CELL BIOLOGY (1988)
GOULD, SAC, FROM ATOMS TO INTEGRATED-CIRCUIT CHIPS, BLOOD-CELLS, AND BACTERIA WITH THE ATOMIC FORCE MICROSCOPE, JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A-VACUUM SURFACES AND FILMS 8: 369 (1990). (10.1116/1.576398) / JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A-VACUUM SURFACES AND FILMS (1990)
HENDERSON, D, STRUCTURE AND BIOCHEMISTRY OF MOUSE HEPATIC GAP-JUNCTIONS, JOURNAL OF MOLECULAR BIOLOGY 132: 193 (1979). (10.1016/0022-2836(79)90391-7) / JOURNAL OF MOLECULAR BIOLOGY (1979)
Hertzberg E. L. Modem Cell Biology: Gap Junctions (1988).
HIROKAWA, N, THE INSIDE AND OUTSIDE OF GAP-JUNCTION MEMBRANES VISUALIZED BY DEEP ETCHING, CELL 30: 395 (1982). (10.1016/0092-8674(82)90237-9) / CELL (1982)
KARRER, H, THE STRIATED MUSCULATURE OF BLOOD VESSELS .2. CELL INTERCONNECTIONS AND CELL SURFACE, JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY 8: 135 (1960). (10.1083/jcb.8.1.135) / JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY (1960)
LAIRD, D.W., BIOCHEMICAL AND IMMUNOCHEMICAL ANALYSIS OF THE ARRANGEMENT OF CONNEXIN43 IN RAT-HEART GAP JUNCTION MEMBRANES, JOURNAL OF CELL SCIENCE 97: 109 (1990). (10.1242/jcs.97.1.109) / JOURNAL OF CELL SCIENCE (1990)
Makowski, L., Gap Junctions: 5 (1985). / Gap Junctions (1985)
MAKOWSKI, L, GAP JUNCTION STRUCTURES .2. ANALYSIS OF X-RAY-DIFFRACTION DATA, JOURNAL OF CELL BIOLOGY 74: 629 (1977). (10.1083/jcb.74.2.629) / JOURNAL OF CELL BIOLOGY (1977)
MANJUNATH, C.K., ISOLATION AND PROTEIN-COMPOSITION OF GAP-JUNCTIONS FROM RABBIT HEARTS, BIOCHEMICAL JOURNAL 205: 189 (1982). (10.1042/bj2050189) / BIOCHEMICAL JOURNAL (1982)
NICHOLSON, B.J., RAT-LIVER GAP JUNCTION PROTEIN - PROPERTIES AND PARTIAL SEQUENCE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 78: 7594 (1981). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1981)
REVEL, J.P., HEXAGONAL ARRAY OF SUBUNITS IN INTERCELLULAR JUNCTIONS OF MOUSE HEART AND LIVER, JOURNAL OF CELL BIOLOGY 33: C7 (1967). (10.1083/jcb.33.3.C7) / JOURNAL OF CELL BIOLOGY (1967)
RUGAR, D, ATOMIC FORCE MICROSCOPY, PHYSICS TODAY 43: 23 (1990). / PHYSICS TODAY (1990)
SOSINSKY, G.E., GAP JUNCTION STRUCTURES .8. MEMBRANE CROSS-SECTIONS, BIOPHYSICAL JOURNAL 53: 709 (1988). (10.1016/S0006-3495(88)83152-7) / BIOPHYSICAL JOURNAL (1988)
SOSINSKY, G.E., CORRELATION-ANALYSIS OF GAP JUNCTION LATTICE IMAGES, BIOPHYSICAL JOURNAL 58: 1213 (1990). (10.1016/S0006-3495(90)82462-0) / BIOPHYSICAL JOURNAL (1990)
UNWIN, PNT, STRUCTURE OF THE JUNCTION BETWEEN COMMUNICATING CELLS, NATURE 283: 545 (1980). (10.1038/283545a0) / NATURE (1980)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 4:45 p.m.)
Indexed	1 month, 2 weeks ago (July 7, 2025, 3:06 a.m.)
Issued	33 years, 11 months ago (Sept. 20, 1991)
Published	33 years, 11 months ago (Sept. 20, 1991)
Published Print	33 years, 11 months ago (Sept. 20, 1991)

Funders 0

None

BibTeX

@article{Hoh_1991, title={Atomic Force Microscopy and Dissection of Gap Junctions}, volume={253}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1910206}, DOI={10.1126/science.1910206}, number={5026}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Hoh, Jan H. and Lal, Ratneshwar and John, Scott A. and Revel, Jean-Paul and Arnsdorf, Morton F.}, year={1991}, month=sep, pages={1405–1408} }

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