DOI: 10.1126/science.1891716. Cloning of Complementary DNA Encoding a Functional Human Interleukin-8 Receptor

Cloning of Complementary DNA Encoding a Functional Human Interleukin-8 Receptor

10.1126/science.1891716

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Interleukin-8 (IL-8) is an inflammatory cytokine that activates neutrophil chemotaxis, degranulation, and the respiratory burst. Neutrophils express receptors for IL-8 that are coupled to guanine nucleotide-binding proteins (G proteins); binding of IL-8 to its receptor induces the mobilization of intracellular calcium stores. A cDNA clone from HL-60 neutrophils, designated p2, has now been isolated that encodes a human IL-8 receptor. When p2 is expressed in oocytes from Xenopus laevis , the oocytes bind 125 I-labeled IL-8 specifically and respond to IL-8 by mobilizing calcium stores with an EC 50 of 20 nM. This IL-8 receptor has 77% amino acid identity with a second human neutrophil receptor isotype that binds IL-8 with higher affinity. It also exhibits 69% amino acid identity with a protein reported to be an N-formyl peptide receptor from rabbit neutrophils, but less than 30% identity with all other known G protein-coupled receptors, including the human N-formyl peptide receptor.

Bibliography

Murphy, P. M., & Tiffany, H. L. (1991). Cloning of Complementary DNA Encoding a Functional Human Interleukin-8 Receptor. Science, 253(5025), 1280â1283.

Authors 2

Philip M. Murphy (first)
H. Lee Tiffany (additional)

References 27 Referenced 694

BESEMER, J, SPECIFIC BINDING, INTERNALIZATION, AND DEGRADATION OF HUMAN NEUTROPHIL ACTIVATING FACTOR BY HUMAN POLYMORPHONUCLEAR LEUKOCYTES, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 17409 (1989). (10.1016/S0021-9258(18)71509-3) / JOURNAL OF BIOLOGICAL CHEMISTRY (1989)
BONNER, T.I., CLONING AND EXPRESSION OF THE HUMAN AND RAT M5 MUSCARINIC ACETYLCHOLINE-RECEPTOR GENES, NEURON 1: 403 (1988). (10.1016/0896-6273(88)90190-0) / NEURON (1988)
BOULAY, F, SYNTHESIS AND USE OF A NOVEL N-FORMYL PEPTIDE DERIVATIVE TO ISOLATE A HUMAN N-FORMYL PEPTIDE RECEPTOR CDNA, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 168: 1103 (1990). (10.1016/0006-291X(90)91143-G) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1990)
10.1093/nar/12.1Part1.387
GERARD, N.P., THE CHEMOTACTIC RECEPTOR FOR HUMAN-C5A ANAPHYLATOXIN, NATURE 349: 614 (1991). (10.1038/349614a0) / NATURE (1991)
GROB, P.M., CHARACTERIZATION OF A RECEPTOR FOR HUMAN MONOCYTE-DERIVED NEUTROPHIL CHEMOTACTIC FACTOR INTERLEUKIN-8, JOURNAL OF BIOLOGICAL CHEMISTRY 265: 8311 (1990). (10.1016/S0021-9258(19)39073-8) / JOURNAL OF BIOLOGICAL CHEMISTRY (1990)
10.1126/science.1840701
10.1126/science.3399891
10.1093/nar/15.20.8125
10.1016/0022-2836(82)90515-0
LEONARD, E.J., LEUKOCYTE SPECIFICITY AND BINDING OF HUMAN NEUTROPHIL ATTRACTANT ACTIVATION PROTEIN-1, JOURNAL OF IMMUNOLOGY 144: 1323 (1990). (10.4049/jimmunol.144.4.1323) / JOURNAL OF IMMUNOLOGY (1990)
MALECH, H.L., ASPARAGINE-LINKED OLIGOSACCHARIDES ON FORMYL PEPTIDE CHEMOTACTIC RECEPTORS OF HUMAN PHAGOCYTIC-CELLS, JOURNAL OF BIOLOGICAL CHEMISTRY 260: 2509 (1985). (10.1016/S0021-9258(18)89582-5) / JOURNAL OF BIOLOGICAL CHEMISTRY (1985)
MOSER, B, NEUTROPHIL-ACTIVATING PEPTIDE-2 AND GRO MELANOMA GROWTH-STIMULATORY ACTIVITY INTERACT WITH NEUTROPHIL-ACTIVATING PEPTIDE-1/INTERLEUKIN-8 RECEPTORS ON HUMAN NEUTROPHILS, JOURNAL OF BIOLOGICAL CHEMISTRY 266: 10666 (1991). (10.1016/S0021-9258(18)99275-6) / JOURNAL OF BIOLOGICAL CHEMISTRY (1991)
MOSER, B, NEUTROPHIL-ACTIVATING PROPERTIES OF THE MELANOMA GROWTH STIMULATORY ACTIVITY, JOURNAL OF EXPERIMENTAL MEDICINE 171: 1797 (1990). (10.1084/jem.171.5.1797) / JOURNAL OF EXPERIMENTAL MEDICINE (1990)
MURPHY, P.M., CHARACTERIZATION OF PHAGOCYTE-P2 NUCLEOTIDE RECEPTORS EXPRESSED IN XENOPUS OOCYTES, JOURNAL OF BIOLOGICAL CHEMISTRY 265: 11615 (1990). (10.1016/S0021-9258(19)38442-X) / JOURNAL OF BIOLOGICAL CHEMISTRY (1990)
MURPHY, P.M., FUNCTIONAL EXPRESSION OF THE HUMAN FORMYL PEPTIDE RECEPTOR IN XENOPUS OOCYTES REQUIRES A COMPLEMENTARY HUMAN FACTOR, JOURNAL OF BIOLOGICAL CHEMISTRY 266: 12560 (1991). (10.1016/S0021-9258(18)98935-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1991)
MURPHY, P.M., CHARACTERIZATION OF HUMAN PHAGOCYTIC CELL RECEPTORS FOR C5A AND PLATELET ACTIVATING FACTOR EXPRESSED IN XENOPUS OOCYTES, JOURNAL OF IMMUNOLOGY 145: 2227 (1990). (10.4049/jimmunol.145.7.2227) / JOURNAL OF IMMUNOLOGY (1990)
MURPHY P.M. unpublished data.
OPPENHEIM, J.J., PROG CLIN BIOL RES 349: 405 (1990). / PROG CLIN BIOL RES (1990)
SAMANTA, A.K., IDENTIFICATION AND CHARACTERIZATION OF SPECIFIC RECEPTORS FOR MONOCYTE-DERIVED NEUTROPHIL CHEMOTACTIC FACTOR (MDNCF) ON HUMAN-NEUTROPHILS, JOURNAL OF EXPERIMENTAL MEDICINE 169: 1185 (1989). (10.1084/jem.169.3.1185) / JOURNAL OF EXPERIMENTAL MEDICINE (1989)
10.1073/pnas.74.12.5463
SULLIVAN, R, ACQUISITION OF FORMYL PEPTIDE RECEPTORS DURING NORMAL HUMAN MYELOID DIFFERENTIATION, BLOOD 70: 1222 (1987). (10.1182/blood.V70.4.1222.1222) / BLOOD (1987)
THELEN, M, MECHANISM OF NEUTROPHIL ACTIVATION BY NAF, A NOVEL MONOCYTE-DERIVED PEPTIDE AGONIST, FASEB JOURNAL 2: 2702 (1988). (10.1096/fasebj.2.11.2840318) / FASEB JOURNAL (1988)
THOMAS, K.M., MOLECULAR-CLONING OF THE FMET-LEU-PHE RECEPTOR FROM NEUTROPHILS, JOURNAL OF BIOLOGICAL CHEMISTRY 265: 20061 (1990). (10.1016/S0021-9258(17)30465-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1990)
WALZ, A, EFFECTS OF THE NEUTROPHIL-ACTIVATING PEPTIDE NAP-2, PLATELET BASIC-PROTEIN, CONNECTIVE TISSUE-ACTIVATING PEPTIDE-III, AND PLATELET FACTOR-4 ON HUMAN-NEUTROPHILS, JOURNAL OF EXPERIMENTAL MEDICINE 170: 1745 (1989). (10.1084/jem.170.5.1745) / JOURNAL OF EXPERIMENTAL MEDICINE (1989)
WOLPE, S.D., MACROPHAGE INFLAMMATORY PROTEIN-1 AND PROTEIN-2 - MEMBERS OF A NOVEL SUPERFAMILY OF CYTOKINES, FASEB JOURNAL 3: 2565 (1989). (10.1096/fasebj.3.14.2687068) / FASEB JOURNAL (1989)
YOKOYAMA, S, ADAPTIVE EVOLUTION OF G-PROTEIN COUPLED RECEPTOR GENES, MOLECULAR BIOLOGY AND EVOLUTION 6: 342 (1989). / MOLECULAR BIOLOGY AND EVOLUTION (1989)

Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 4:49 p.m.)
Indexed	5 days, 15 hours ago (Aug. 31, 2025, 6:34 a.m.)
Issued	33 years, 11 months ago (Sept. 13, 1991)
Published	33 years, 11 months ago (Sept. 13, 1991)
Published Print	33 years, 11 months ago (Sept. 13, 1991)

Funders 0

None

BibTeX

@article{Murphy_1991, title={Cloning of Complementary DNA Encoding a Functional Human Interleukin-8 Receptor}, volume={253}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1891716}, DOI={10.1126/science.1891716}, number={5025}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Murphy, Philip M. and Tiffany, H. Lee}, year={1991}, month=sep, pages={1280–1283} }

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    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1991,
        9,
        13
      ]
    ]
  }
}