DOI: 10.1126/science.1887216. Differential Phosphorylation of the Transcription Factor Oct1 During the Cell Cycle

Differential Phosphorylation of the Transcription Factor Oct1 During the Cell Cycle

10.1126/science.1887216

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Orderly progression through the somatic cell division cycle is accompanied by phase-specific transcription of a variety of different genes. During S phase, transcription of mammalian histone H2B genes requires a specific promoter element and its cognate transcription factor Oct1 (OTF1). A possible mechanism for regulating histone H2B transcription during the cell cycle is direct modulation of Oct1 activity by phase-specific posttranslational modifications. Analysis of Oct1 during progression through the cell cycle revealed a complex temporal program of phosphorylation. A p34 cdc2 -related protein kinase that is active during mitosis may be responsible for one mitotic phosphorylation of Oct1. However, the temporally controlled appearance of Oct1 phosphopeptides suggests the involvement of multiple kinases and phosphatases. These results support the idea that cell cycle-regulated transcription factors may be direct substrates for phase-specific regulatory enzymes.

Bibliography

Roberts, S. B., Segil, N., & Heintz, N. (1991). Differential Phosphorylation of the Transcription Factor Oct1 During the Cell Cycle. Science, 253(5023), 1022â1026.

Authors 3

Susan Boseman Roberts (first)
Neil Segil (additional)
Nathaniel Heintz (additional)

References 48 Referenced 102

ARTISHEVSKY, A, USE OF A CELL-CYCLE MUTANT TO DELINEATE THE CRITICAL PERIOD FOR THE CONTROL OF HISTONE MESSENGER-RNA LEVELS IN THE MAMMALIAN-CELL CYCLE, MOLECULAR AND CELLULAR BIOLOGY 4: 2364 (1984). / MOLECULAR AND CELLULAR BIOLOGY (1984)
10.1038/328823a0
10.1002/j.1460-2075.1987.tb02676.x
10.1002/j.1460-2075.1988.tb02782.x
DALTON, S, MAXIMAL BINDING LEVELS OF AN H-1 HISTONE GENE-SPECIFIC FACTOR IN S-PHASE CORRELATE WITH MAXIMAL H-1 GENE-TRANSCRIPTION, MOLECULAR AND CELLULAR BIOLOGY 8: 4576 (1988). / MOLECULAR AND CELLULAR BIOLOGY (1988)
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Dunbar B. S. Two-Dimensional Electrophoresis and Immunological Techniques (1987). (10.1007/978-1-4613-1957-3)
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GALLINARI, P, CHARACTERIZATION AND PURIFICATION OF H1TF2, A NOVEL CCAAT-BINDING PROTEIN THAT INTERACTS WITH A HISTONE-H1 SUBTYPE-SPECIFIC CONSENSUS ELEMENT, MOLECULAR AND CELLULAR BIOLOGY 9: 1566 (1989). / MOLECULAR AND CELLULAR BIOLOGY (1989)
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Harlow E. Antibodies: A Laboratory Manual (1988).
10.1126/science.2683079
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10.1016/0092-8674(90)90470-Y
HEINTZ, N, REGULATION OF HUMAN HISTONE GENE-EXPRESSION - KINETICS OF ACCUMULATION AND CHANGES IN THE RATE OF SYNTHESIS AND IN THE HALF-LIVES OF INDIVIDUAL HISTONE MESSENGER-RNAS DURING THE HELA-CELL CYCLE, MOLECULAR AND CELLULAR BIOLOGY 3: 539 (1983). / MOLECULAR AND CELLULAR BIOLOGY (1983)
HEINTZ, N, TRANSCRIPTION OF HUMAN HISTONE GENES IN EXTRACTS FROM SYNCHRONIZED HELA-CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 81: 2713 (1984). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1984)
HEMMINGS, H.C., DARPP-32, A DOPAMINE-MONOPHOSPHATE-REGULATED AND ADENOSINE 3'-5'-MONOPHOSPHATE-REGULATED NEURONAL PHOSPHOPROTEIN .2. COMPARISON OF THE KINETICS OF PHOSPHORYLATION OF DARPP-32 AND PHOSPHATASE INHIBITOR-1, JOURNAL OF BIOLOGICAL CHEMISTRY 259: 4491 (1984). / JOURNAL OF BIOLOGICAL CHEMISTRY (1984)
10.1016/0092-8674(82)90036-8
JOHNSON, D.G., THE UBIQUITOUS OCTAMER-BINDING PROTEIN(S) IS SUFFICIENT FOR TRANSCRIPTION OF IMMUNOGLOBULIN GENES, MOLECULAR AND CELLULAR BIOLOGY 10: 982 (1990). / MOLECULAR AND CELLULAR BIOLOGY (1990)
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NUSSE, M, CAN NOCODAZOLE, AN INHIBITOR OF MICROTUBULE FORMATION, BE USED TO SYNCHRONIZE MAMMALIAN-CELLS - ACCUMULATION OF CELLS IN MITOSIS STUDIED BY 2 PARAMETRIC FLOW-CYTOMETRY USING ACRIDINE-ORANGE AND BY DNA DISTRIBUTION ANALYSIS, CELL AND TISSUE KINETICS 17: 13 (1984). / CELL AND TISSUE KINETICS (1984)
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10.1101/gad.4.1.9
10.1016/0092-8674(90)90179-I
ROBERTS S unpublished data.
SEGIL N unpublished data.
SITTMAN, D.B., HISTONE MESSENGER-RNA CONCENTRATIONS ARE REGULATED AT THE LEVEL OF TRANSCRIPTION AND MESSENGER-RNA DEGRADATION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 80: 1849 (1983). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1983)
10.1073/pnas.83.17.6382
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10.1016/0014-4827(80)90279-7

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 3:37 p.m.)
Indexed	3 months, 1 week ago (May 22, 2025, 2:46 a.m.)
Issued	34 years ago (Aug. 30, 1991)
Published	34 years ago (Aug. 30, 1991)
Published Print	34 years ago (Aug. 30, 1991)

Funders 0

None

BibTeX

@article{Roberts_1991, title={Differential Phosphorylation of the Transcription Factor Oct1 During the Cell Cycle}, volume={253}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1887216}, DOI={10.1126/science.1887216}, number={5023}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Roberts, Susan Boseman and Segil, Neil and Heintz, Nathaniel}, year={1991}, month=aug, pages={1022–1026} }

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