DOI: 10.1126/science.1857986. Protein Kinase Activity Closely Associated with a Reconstituted Calcium-Activated Potassium Channel

Protein Kinase Activity Closely Associated with a Reconstituted Calcium-Activated Potassium Channel

10.1126/science.1857986

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Modulation of the activity of potassium and other ion channels is an essential feature of nervous system function. The open probability of a large conductance Ca 2+ -activated K + channel from rat brain, incorporated into planar lipid bilayers, is increased by the addition of adenosine triphosphate (ATP) to the cytoplasmic side of the channel. This modulation takes place without the addition of protein kinase, requires Mg 2+ , and is mimicked by an ATP analog that serves as a substrate for protein kinases but not by a nonhydrolyzable ATP analog. Addition of protein phosphatase 1 reverses the modulation by MgATP. Thus, there may be an endogenous protein kinase activity firmly associated with this K + channel. Some ion channels may exist in a complex that contains regulatory protein kinases and phosphatases.

Bibliography

Chung, S., Reinhart, P. H., Martin, B. L., Brautigan, D., & Levitan, I. B. (1991). Protein Kinase Activity Closely Associated with a Reconstituted Calcium-Activated Potassium Channel. Science, 253(5019), 560â562.

Authors 5

Sungkwon Chung (first)
Peter H. Reinhart (additional)
Bruce L. Martin (additional)
David Brautigan (additional)
Irwin B. Levitan (additional)

References 28 Referenced 132

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 4:25 p.m.)
Indexed	1 month, 3 weeks ago (July 6, 2025, 1:44 p.m.)
Issued	34 years, 1 month ago (Aug. 2, 1991)
Published	34 years, 1 month ago (Aug. 2, 1991)
Published Print	34 years, 1 month ago (Aug. 2, 1991)

Funders 0

None

BibTeX

@article{Chung_1991, title={Protein Kinase Activity Closely Associated with a Reconstituted Calcium-Activated Potassium Channel}, volume={253}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1857986}, DOI={10.1126/science.1857986}, number={5019}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Chung, Sungkwon and Reinhart, Peter H. and Martin, Bruce L. and Brautigan, David and Levitan, Irwin B.}, year={1991}, month=aug, pages={560–562} }

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