DOI: 10.1126/science.185.4148.357. Assembly of Chick Brain Tubulin onto Isolated Basal Bodies of <i>Chlamydomonas reinhardi</i>

Assembly of Chick Brain Tubulin onto Isolated Basal Bodies of Chlamydomonas reinhardi

10.1126/science.185.4148.357

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Basal bodies isolated from Chlamydomonas reinhardi will serve as initiation centers for the assembly of chick brain microtubule protein subunits (tubulin) into microtubules. The rate of microtubule assembly is tubulin-concentration dependent; this assembly occurs onto both distal and proximal ends of the basal body microtubules, with distal assembly greatly favored. In vitro assembly of brain tubulin also occurs onto the mid-lateral aspects of the basal bodies, presumably onto the fiber connecting the two basal bodies.

Bibliography

Snell, W. J., Dentler, W. L., Haimo, L. T., Binder, L. I., & Rosenbaum, J. L. (1974). Assembly of Chick Brain Tubulin onto Isolated Basal Bodies of Chlamydomonas reinhardi. Science, 185(4148), 357â360.

Authors 5

W. J. Snell (first)
W. L. Dentler (additional)
L. T. Haimo (additional)
L. I. Binder (additional)
J. L. Rosenbaum (additional)

References 30 Referenced 81

ALLEN, C, J CELL BIOL 49: 9A (1973). / J CELL BIOL (1973)
ALLEN C PERSONAL COMMUNICATION.
ANDERSON, R.G., ISOLATION OF CILIATED OR UNCILIATED BASAL BODIES FROM RABBIT OVIDUCT, JOURNAL OF CELL BIOLOGY 60: 393 (1974). (10.1083/jcb.60.2.393) / JOURNAL OF CELL BIOLOGY (1974)
Binder, L. I., Directionality of assemby of chick brain tubulin onto sea urchin flagella microtubules, Biological Bulletin 145: 424 (1973). / Biological Bulletin / Directionality of assemby of chick brain tubulin onto sea urchin flagella microtubules (1973)
Binder L. I. in preparation.
BINDER, L. I., JOURNAL OF CELL BIOLOGY 59: A24 (1973). / JOURNAL OF CELL BIOLOGY (1973)
CANDE, W.Z., FUNCTIONAL MITOTIC SPINDLE PREPARED FROM MAMMALIAN-CELLS IN CULTURE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 71: 1559 (1974). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1974)
CLAES, H, AUTOLYSIS OF CELL WALL FROM GAMETES OF CHLAMYDOMONAS-REINHARDII, ARCHIV FUR MIKROBIOLOGIE 78: 180 (1971). (10.1007/BF00424874) / ARCHIV FUR MIKROBIOLOGIE (1971)
CLARK W PERSONAL COMMUNICATION.
COYNE, B, FLAGELLAR ELONGATION AND SHORTENING IN CHLAMYDOMONAS .2. RE-UTILIZATION OF FLAGELLAR PROTEINS, JOURNAL OF CELL BIOLOGY 47: 777 (1970). (10.1083/jcb.47.3.777) / JOURNAL OF CELL BIOLOGY (1970)
DENTLER, W.L., DIRECTIONALITY OF BRAIN MICROTUBULE ASSEMBLY INVITRO, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 71: 1710 (1974). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1974)
FRIEDMANN, I, FINE-STRUCTURAL ASPECTS OF FERTILIZATION IN CHLAMYDOMONAS REINHARDI, JOURNAL OF CELL SCIENCE 3: 115 (1968). (10.1242/jcs.3.1.115) / JOURNAL OF CELL SCIENCE (1968)
Fulton C. Origin and Continuity of Cell Organelles (1971).
GILULA, N.B., CILIARY NECKLACE - CILIARY MEMBRANE SPECIALIZATION, JOURNAL OF CELL BIOLOGY 53: 494 (1972). (10.1083/jcb.53.2.494) / JOURNAL OF CELL BIOLOGY (1972)
GOULD R PERSONAL COMMUNICATION.
Inoue, S., Isolation and growth of a cold and calcium labile spindle from Chaetopterus oocytes in solutions containing pig brain tubilin, Biological Bulletin 145: 441 (1973). / Biological Bulletin / Isolation and growth of a cold and calcium labile spindle from Chaetopterus oocytes in solutions containing pig brain tubilin (1973)
JOHNSON, U.G., FINE STRUCTURE OF CELL DIVISION IN CHLAMYDOMONAS REINHARDI - BASAL BODIES AND MICROTUBULES, JOURNAL OF CELL BIOLOGY 38: 403 (1968). (10.1083/jcb.38.2.403) / JOURNAL OF CELL BIOLOGY (1968)
KATES, J.R., CONTROL OF GAMETIC DIFFERENTIATION IN LIQUID CULTURES OF CHLAMYDOMONAS, JOURNAL OF CELLULAR AND COMPARATIVE PHYSIOLOGY 63: 157 (1964). (10.1002/jcp.1030630204) / JOURNAL OF CELLULAR AND COMPARATIVE PHYSIOLOGY (1964)
PICKETTHEAPS, J.D., CYTOBIOS 3: 257 (1969). / CYTOBIOS (1969)
REBHUN, L.I., REVERSIBLE RESTORATION OF BIREFRINGENCE OF COLD-TREATED, ISOLATED MITOTIC APPARATUS OF SURF CLAM EGGS WITH CHICK BRAIN TUBULIN, NATURE 249: 113 (1974). (10.1038/249113a0) / NATURE (1974)
RINGO, D.L., FLAGELLAR MOTION AND FINE STRUCTURE OF FLAGELLAR APPARATUS IN CHLAMYDOMONAS, JOURNAL OF CELL BIOLOGY 33: 543 (1967). (10.1083/jcb.33.3.543) / JOURNAL OF CELL BIOLOGY (1967)
ROSENBAUM, J.L., FLAGELLAR REGENERATION IN PROTOZOAN FLAGELLATES, JOURNAL OF CELL BIOLOGY 34: 345 (1967). (10.1083/jcb.34.1.345) / JOURNAL OF CELL BIOLOGY (1967)
ROSENBAUM, J.L., FLAGELLAR ELONGATION AND SHORTENING IN CHLAMYDOMONAS - USE OF CYCLOHEXIMIDE AND COLCHICINE TO STUDY SYNTHESIS AND ASSEMBLY OF FLAGELLAR PROTEINS, JOURNAL OF CELL BIOLOGY 41: 600 (1969). (10.1083/jcb.41.2.600) / JOURNAL OF CELL BIOLOGY (1969)
RUBIN, R.W., PARTIAL-PURIFICATION AND PHOSPHOTUNGSTATE SOLUBILIZATION OF BASAL BODIES AND KINETODESMAL FIBERS FROM TETRAHYMENA-PYRIFORMIS, JOURNAL OF CELL BIOLOGY 57: 601 (1973). (10.1083/jcb.57.3.601) / JOURNAL OF CELL BIOLOGY (1973)
SAGER, R, NUTRITIONAL CONTROL OF SEXUALITY IN CHLAMYDOMONAS REINHARDI, JOURNAL OF GENERAL PHYSIOLOGY 37: 729 (1954). (10.1085/jgp.37.6.729) / JOURNAL OF GENERAL PHYSIOLOGY (1954)
10.1126/science.177.4054.1104
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WITMAN, G.B., CHLAMYDOMONAS FLAGELLA .1. ISOLATION AND ELECTROPHORETIC ANALYSIS OF MICROTUBULES, MATRIX, MEMBRANES, AND MASTIGONEMES, JOURNAL OF CELL BIOLOGY 54: 507 (1972). (10.1083/jcb.54.3.507) / JOURNAL OF CELL BIOLOGY (1972)
WITMAN, G, JOURNAL OF CELL BIOLOGY 59: A366 (1973). / JOURNAL OF CELL BIOLOGY (1973)
WOLFE J ADV CELL MOLECULAR B 2 (1972).

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 11:10 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 4:52 p.m.)
Indexed	1 day, 4 hours ago (Sept. 3, 2025, 6:54 a.m.)
Issued	51 years, 1 month ago (July 26, 1974)
Published	51 years, 1 month ago (July 26, 1974)
Published Print	51 years, 1 month ago (July 26, 1974)

Funders 0

None

BibTeX

@article{Snell_1974, title={Assembly of Chick Brain Tubulin onto Isolated Basal Bodies of Chlamydomonas reinhardi}, volume={185}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.185.4148.357}, DOI={10.1126/science.185.4148.357}, number={4148}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Snell, W. J. and Dentler, W. L. and Haimo, L. T. and Binder, L. I. and Rosenbaum, J. L.}, year={1974}, month=jul, pages={357–360} }

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