DOI: 10.1126/science.184.4132.77. Electron Diffraction of Wet Biological Membranes

Electron Diffraction of Wet Biological Membranes

10.1126/science.184.4132.77

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Electron diffraction patterns were obtained for the first time from single wet phospholipid bilayers and from wet human erythrocyte membranes by using a temperature-controlled electron microscope hydration stage. Selective area diffraction showed the existence of semicrystalline domains. A structural transition was observed at the transition temperature of the wet dipalmitoyl lecithin bilayer.

Bibliography

Hui, S. W., & Parsons, D. F. (1974). Electron Diffraction of Wet Biological Membranes. Science, 184(4132), 77â78.

Authors 2

S. W. Hui (first)
D. F. Parsons (additional)

References 14 Referenced 54

BERNENGO, J.C., HIGH-ANGLE X-RAY-DIFFRACTION STUDY OF RED-CELL MEMBRANES AFTER TREATMENT WITH PHOSPHOLIPASES-A2 AND C, CANADIAN JOURNAL OF BIOCHEMISTRY 50: 1260 (1972). (10.1139/o72-171) / CANADIAN JOURNAL OF BIOCHEMISTRY (1972)
Castaing, R., Proceedings of the 31st Electron Microscopy Society of America: 282 (1973). / Proceedings of the 31st Electron Microscopy Society of America (1973)
CHALCROFT, J.P., AN INTERPRETATION OF LIVER CELL MEMBRANE AND JUNCTION STRUCTURE BASED ON OBSERVATION OF FREEZE-FRACTURE REPLICAS OF BOTH SIDES OF FRACTURE, JOURNAL OF CELL BIOLOGY 47: 49 (1970). (10.1083/jcb.47.1.49) / JOURNAL OF CELL BIOLOGY (1970)
CHAPMAN, D, CHEM PHYS LIPIDS 1: 445 (1967). (10.1016/0009-3084(67)90023-0) / CHEM PHYS LIPIDS (1967)
Chapman, D., The Structure of Lipids: 291 (1965). / The Structure of Lipids (1965)
GOODENOUGH, D.A., ISOLATION OF MOUSE HEPATOCYTE GAP JUNCTIONS - PRELIMINARY CHEMICAL CHARACTERIZATION AND X-RAY-DIFFRACTION, JOURNAL OF CELL BIOLOGY 54: 646 (1972). (10.1083/jcb.54.3.646) / JOURNAL OF CELL BIOLOGY (1972)
Hinkelman, R. M., Proceedings of the 31st Electron Microscopy Society of America: 288 (1973). / Proceedings of the 31st Electron Microscopy Society of America (1973)
Hui S. W. in preparation.
Hui, S.W., Proceedings of the 31st Electron Microscopy Society of America: 340 (1973). / Proceedings of the 31st Electron Microscopy Society of America (1973)
JUNG, C.Y., EVIDENCE OF HIGH STABILITY OF GLUCOSE TRANSPORT CARRIER FUNCTION IN HUMAN RED CELL GHOSTS EXTENSIVELY WASHED IN VARIOUS MEDIA, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 146: 215 (1971). (10.1016/S0003-9861(71)80058-9) / ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (1971)
Levine, Y. K., Nature 220:577 (1968). (10.1038/220577a0) / Nature (1968)
MARCHESI, V.T., MOLECULAR FEATURES OF MAJOR GLYCOPROTEIN OF HUMAN ERYTHROCYTE-MEMBRANE, FEDERATION PROCEEDINGS 32: 1833 (1973). / FEDERATION PROCEEDINGS (1973)
MATRICARDI, V.R., MICRON 3: 526 (1972). / MICRON (1972)
Parsons, D. F., Advances in Medical and Biological Physics 15: 161 (1973). / Advances in Medical and Biological Physics (1973)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 11:10 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 4:23 p.m.)
Indexed	3 months, 2 weeks ago (May 12, 2025, 10:25 a.m.)
Issued	51 years, 4 months ago (April 5, 1974)
Published	51 years, 4 months ago (April 5, 1974)
Published Print	51 years, 4 months ago (April 5, 1974)

Funders 0

None

BibTeX

@article{Hui_1974, title={Electron Diffraction of Wet Biological Membranes}, volume={184}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.184.4132.77}, DOI={10.1126/science.184.4132.77}, number={4132}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Hui, S. W. and Parsons, D. F.}, year={1974}, month=apr, pages={77–78} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        5,
        12
      ]
    ],
    "date-time": "2025-05-12T14:25:22Z",
    "timestamp": 1747059922979
  },
  "reference-count": 14,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "4132",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1974,
        4,
        5
      ]
    ]
  },
  "abstract": "<jats:p>Electron diffraction patterns were obtained for the first time from single wet phospholipid bilayers and from wet human erythrocyte membranes by using a temperature-controlled electron microscope hydration stage. Selective area diffraction showed the existence of semicrystalline domains. A structural transition was observed at the transition temperature of the wet dipalmitoyl lecithin bilayer.</jats:p>",
  "DOI": "10.1126/science.184.4132.77",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        10,
        5
      ]
    ],
    "date-time": "2006-10-05T15:10:48Z",
    "timestamp": 1160061048000
  },
  "page": "77-78",
  "source": "Crossref",
  "is-referenced-by-count": 54,
  "title": "Electron Diffraction of Wet Biological Membranes",
  "prefix": "10.1126",
  "volume": "184",
  "author": [
    {
      "given": "S. W.",
      "family": "Hui",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Electron Optics Laboratory, Roswell Park Memorial Institute, Buffalo, New, York 14203"
        }
      ]
    },
    {
      "given": "D. F.",
      "family": "Parsons",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Electron Optics Laboratory, Roswell Park Memorial Institute, Buffalo, New, York 14203"
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_2_1_1_1",
      "doi-asserted-by": "crossref",
      "first-page": "1260",
      "DOI": "10.1139/o72-171",
      "volume": "50",
      "year": "1972",
      "unstructured": "BERNENGO, J.C., HIGH-ANGLE X-RAY-DIFFRACTION STUDY OF RED-CELL MEMBRANES AFTER TREATMENT WITH PHOSPHOLIPASES-A2 AND C, CANADIAN JOURNAL OF BIOCHEMISTRY 50: 1260 (1972).",
      "journal-title": "CANADIAN JOURNAL OF BIOCHEMISTRY"
    },
    {
      "key": "e_1_2_1_2_1",
      "first-page": "282",
      "year": "1973",
      "unstructured": "Castaing, R., Proceedings of the 31st Electron Microscopy Society of America: 282 (1973).",
      "journal-title": "Proceedings of the 31st Electron Microscopy Society of America"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "crossref",
      "first-page": "49",
      "DOI": "10.1083/jcb.47.1.49",
      "volume": "47",
      "year": "1970",
      "unstructured": "CHALCROFT, J.P., AN INTERPRETATION OF LIVER CELL MEMBRANE AND JUNCTION STRUCTURE BASED ON OBSERVATION OF FREEZE-FRACTURE REPLICAS OF BOTH SIDES OF FRACTURE, JOURNAL OF CELL BIOLOGY 47: 49 (1970).",
      "journal-title": "JOURNAL OF CELL BIOLOGY"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "crossref",
      "first-page": "445",
      "DOI": "10.1016/0009-3084(67)90023-0",
      "volume": "1",
      "year": "1967",
      "unstructured": "CHAPMAN, D, CHEM PHYS LIPIDS 1: 445 (1967).",
      "journal-title": "CHEM PHYS LIPIDS"
    },
    {
      "key": "e_1_2_1_5_1",
      "first-page": "291",
      "year": "1965",
      "unstructured": "Chapman, D., The Structure of Lipids: 291 (1965).",
      "journal-title": "The Structure of Lipids"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "crossref",
      "first-page": "646",
      "DOI": "10.1083/jcb.54.3.646",
      "volume": "54",
      "year": "1972",
      "unstructured": "GOODENOUGH, D.A., ISOLATION OF MOUSE HEPATOCYTE GAP JUNCTIONS - PRELIMINARY CHEMICAL CHARACTERIZATION AND X-RAY-DIFFRACTION, JOURNAL OF CELL BIOLOGY 54: 646 (1972).",
      "journal-title": "JOURNAL OF CELL BIOLOGY"
    },
    {
      "key": "e_1_2_1_7_1",
      "first-page": "288",
      "year": "1973",
      "unstructured": "Hinkelman, R. M., Proceedings of the 31st Electron Microscopy Society of America: 288 (1973).",
      "journal-title": "Proceedings of the 31st Electron Microscopy Society of America"
    },
    {
      "key": "e_1_2_1_8_1",
      "unstructured": "Hui S. W. in preparation."
    },
    {
      "key": "e_1_2_1_9_1",
      "first-page": "340",
      "year": "1973",
      "unstructured": "Hui, S.W., Proceedings of the 31st Electron Microscopy Society of America: 340 (1973).",
      "journal-title": "Proceedings of the 31st Electron Microscopy Society of America"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "crossref",
      "first-page": "215",
      "DOI": "10.1016/S0003-9861(71)80058-9",
      "volume": "146",
      "year": "1971",
      "unstructured": "JUNG, C.Y., EVIDENCE OF HIGH STABILITY OF GLUCOSE TRANSPORT CARRIER FUNCTION IN HUMAN RED CELL GHOSTS EXTENSIVELY WASHED IN VARIOUS MEDIA, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 146: 215 (1971).",
      "journal-title": "ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "crossref",
      "first-page": "577",
      "DOI": "10.1038/220577a0",
      "volume": "220",
      "year": "1968",
      "unstructured": "Levine, Y. K., Nature 220:577 (1968).",
      "journal-title": "Nature"
    },
    {
      "key": "e_1_2_1_12_1",
      "first-page": "1833",
      "volume": "32",
      "year": "1973",
      "unstructured": "MARCHESI, V.T., MOLECULAR FEATURES OF MAJOR GLYCOPROTEIN OF HUMAN ERYTHROCYTE-MEMBRANE, FEDERATION PROCEEDINGS 32: 1833 (1973).",
      "journal-title": "FEDERATION PROCEEDINGS"
    },
    {
      "key": "e_1_2_1_13_1",
      "first-page": "526",
      "volume": "3",
      "year": "1972",
      "unstructured": "MATRICARDI, V.R., MICRON 3: 526 (1972).",
      "journal-title": "MICRON"
    },
    {
      "key": "e_1_2_1_14_1",
      "first-page": "161",
      "volume": "15",
      "year": "1973",
      "unstructured": "Parsons, D. F., Advances in Medical and Biological Physics 15: 161 (1973).",
      "journal-title": "Advances in Medical and Biological Physics"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.184.4132.77",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        11
      ]
    ],
    "date-time": "2024-01-11T21:23:35Z",
    "timestamp": 1705008215000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.184.4132.77"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1974,
        4,
        5
      ]
    ]
  },
  "references-count": 14,
  "journal-issue": {
    "issue": "4132",
    "published-print": {
      "date-parts": [
        [
          1974,
          4,
          5
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.184.4132.77"
  ],
  "URL": "http://dx.doi.org/10.1126/science.184.4132.77",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1974,
        4,
        5
      ]
    ]
  }
}