DOI: 10.1126/science.182.4114.825. Collagen Synthesis: Localization of Prolyl Hydroxylase in Tendon Cells Detected with Ferritin-Labeled Antibodies

Collagen Synthesis: Localization of Prolyl Hydroxylase in Tendon Cells Detected with Ferritin-Labeled Antibodies

10.1126/science.182.4114.825

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

An improved procedure was employed for linking ferritin to antibodies against prolyl hydroxylase, the enzyme that synthesizes the hydroxyproline in collagen. By electron microscopy, the enzyme was then found to be localized in cisternae of the rough endoplasmic reticulum of embryonic tendon cells; this indicates that hydroxylation of proline occurs while newly synthesized polypeptides are fed into the cisternae.

Bibliography

Olsen, B. R., Berg, R. A., Kishida, Y., & Prockop, D. J. (1973). Collagen Synthesis: Localization of Prolyl Hydroxylase in Tendon Cells Detected with Ferritin-Labeled Antibodies. Science, 182(4114), 825â827.

Authors 4

Bjørn R. Olsen (first)
Richard A. Berg (additional)
Yasuo Kishida (additional)
Darwin J. Prockop (additional)

References 25 Referenced 78

AINSWORTH, S.K., ULTRASTRUCTURAL STAINING METHOD FOR ENHANCING SIZE AND ELECTRON OPACITY OF FERRITIN IN THIN SECTIONS, JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 20: 225 (1972). (10.1177/20.3.225) / JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY (1972)
AVRAMEAS, S, ENZYME MARKERS - THEIR LINKAGE WITH PROTEINS AND USE IN IMMUNO-HISTOCHEMISTRY, HISTOCHEMICAL JOURNAL 4: 321 (1972). (10.1007/BF01005007) / HISTOCHEMICAL JOURNAL (1972)
BERG, R.A., PURIFICATION OF [C-14]PROTOCOLLAGEN AND ITS HYDROXYLATION BY PROLYL-HYDROXYLASE, BIOCHEMISTRY 12: 3395 (1973). (10.1021/bi00742a005) / BIOCHEMISTRY (1973)
BERG, R.A., LABELED ANTIBODIES TO PROTOCOLLAGEN PROLINE HYDROXYLASE FROM CHICK-EMBRYOS FOR INTRACELLULAR LOCALIZATION OF ENZYME, BIOCHIMICA ET BIOPHYSICA ACTA 285: 167 (1972). (10.1016/0005-2795(72)90188-2) / BIOCHIMICA ET BIOPHYSICA ACTA (1972)
BERG, R.A., AFFINITY COLUMN PURIFICATION OF PROTOCOLLAGEN PROLINE HYDROXYLASE FROM CHICK-EMBRYOS AND FURTHER CHARACTERIZATION OF ENZYME, JOURNAL OF BIOLOGICAL CHEMISTRY 248: 1175 (1973). (10.1016/S0021-9258(19)44278-6) / JOURNAL OF BIOLOGICAL CHEMISTRY (1973)
Berg, R. A., Abstracts of the Ninth International Congress of Biochemistry: 423 (1973). / Abstracts of the Ninth International Congress of Biochemistry (1973)
COOPER, G.W., INTRACELLULAR ACCUMULATION OF PROTOCOLLAGEN AND EXTRUSION OF COLLAGEN BY EMBRYONIC CARTILAGE CELLS, JOURNAL OF CELL BIOLOGY 38: 523 (1968). (10.1083/jcb.38.3.523) / JOURNAL OF CELL BIOLOGY (1968)
CUATRECASAS, P, PROTEIN PURIFICATION BY AFFINITY CHROMATOGRAPHY - DERIVATIZATIONS OFAGAROSE AND POLYACRYLAMIDE BEADS, JOURNAL OF BIOLOGICAL CHEMISTRY 245: 3059 (1970). (10.1016/S0021-9258(18)63022-4) / JOURNAL OF BIOLOGICAL CHEMISTRY (1970)
DEHM, P, SYNTHESIS AND EXTRUSION OF COLLAGEN BY FRESHLY ISOLATED CELLS FROM CHICK EMBRYO TENDON, BIOCHIMICA ET BIOPHYSICA ACTA 240: 358 (1971). (10.1016/0005-2787(71)90529-6) / BIOCHIMICA ET BIOPHYSICA ACTA (1971)
ESKELAND, T, CHARACTERIZATION OF IMMUNOGLOBULIN STRUCTURES FROM SURFACE OF CHRONIC LYMPHOCYTIC LEUKEMIA CELLS, JOURNAL OF EXPERIMENTAL MEDICINE 134: 265 (1971). (10.1084/jem.134.1.265) / JOURNAL OF EXPERIMENTAL MEDICINE (1971)
GRANT, M.E., BIOSYNTHESIS OF COLLAGEN .1., NEW ENGLAND JOURNAL OF MEDICINE 286: 194 (1972). (10.1056/NEJM197201272860406) / NEW ENGLAND JOURNAL OF MEDICINE (1972)
HAY, E.D., SECRETION OF COLLAGEN BY CORNEAL EPITHELIUM .1. MORPHOLOGY OF COLLAGENOUS PRODUCTS PRODUCED BY ISOLATED EPITHELIA GROWN ON FROZEN-KILLED LENS, JOURNAL OF CELL BIOLOGY 57: 190 (1973). (10.1083/jcb.57.1.190) / JOURNAL OF CELL BIOLOGY (1973)
HIRANO, H, DISTRIBUTION OF SACCHARIDE RESIDUES ON MEMBRANE FRAGMENTS FROM A MYELOMA-CELL HOMOGENATE - ITS IMPLICATIONS FOR MEMBRANE BIOGENESIS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 69: 2945 (1972). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1972)
JAMIESON, J.D., INTRACELLULAR TRANSPORT OF SECRETORY PROTEINS IN PANCREATIC EXOCRINE CELL .I. ROLE OF PERIPHERAL ELEMENTS OF GOLGI COMPLEX, JOURNAL OF CELL BIOLOGY 34: 577 (1967). (10.1083/jcb.34.2.577) / JOURNAL OF CELL BIOLOGY (1967)
JIMENEZ, S.A., INTRACELLULAR COLLAGEN AND PROTOCOLLAGEN FROM EMBRYONIC TENDON CELLS, JOURNAL OF BIOLOGICAL CHEMISTRY 248: 720 (1973). (10.1016/S0021-9258(19)44432-3) / JOURNAL OF BIOLOGICAL CHEMISTRY (1973)
10.1126/science.152.3718.92
Kishida Y. in preparation.
KRAEHENBUHL, J.P., SOLID-PHASE CONJUGATION OF FERRITIN TO FAB-FRAGMENTS OF IMMUNOGLOBULIN-G IN G FOR USE IN ANTIGEN LOCALIZATION ON THIN SECTIONS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 69: 1771 (1972). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1972)
Olsen B. R. in preparation.
ROSS, R, WOUND HEALING AND COLLAGEN FORMATION .V. QUANTITATIVE ELECTRON MICROSCOPE RADIOAUTOGRAPHIC OBSERVATIONS OF PROLINE-H3 UTILIZATION BY FIBROBLASTS, JOURNAL OF CELL BIOLOGY 27: 83 (1965). (10.1083/jcb.27.1.83) / JOURNAL OF CELL BIOLOGY (1965)
SALPETER, M.M., H3-PROLINE INCORPORATION INTO CARTILAGE - ELECTRON MICROSCOPE AUTORADIOGRAPHIC OBSERVATIONS, JOURNAL OF MORPHOLOGY 124: 387 (1968). (10.1002/jmor.1051240402) / JOURNAL OF MORPHOLOGY (1968)
SAUSSURE, VAD, IMMUNOCHEMISTRY 6: 77 (1969). (10.1016/0019-2791(69)90180-3) / IMMUNOCHEMISTRY (1969)
TRELSTAD, R.L., GOLGI APPARATUS IN CHICK CORNEAL EPITHELIUM - CHANGES IN INTRACELLULAR POSITION DURING DEVELOPMENT, JOURNAL OF CELL BIOLOGY 45: 34 (1970). (10.1083/jcb.45.1.34) / JOURNAL OF CELL BIOLOGY (1970)
Uitto, J., Abstracts of the Ninth International Congress of Biochemistry: 425 (1973). / Abstracts of the Ninth International Congress of Biochemistry (1973)
WEINSTOCK, M, COLLAGEN FORMATION - OBSERVATIONS ON ITS INTRACELLULAR PACKAGING AND TRANSPORT, ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE 129: 455 (1972). (10.1007/BF00316743) / ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE (1972)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 10:05 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 3:52 p.m.)
Indexed	1 year, 7 months ago (Jan. 11, 2024, 7:03 p.m.)
Issued	51 years, 9 months ago (Nov. 23, 1973)
Published	51 years, 9 months ago (Nov. 23, 1973)
Published Print	51 years, 9 months ago (Nov. 23, 1973)

Funders 0

None

BibTeX

@article{Olsen_1973, title={Collagen Synthesis: Localization of Prolyl Hydroxylase in Tendon Cells Detected with Ferritin-Labeled Antibodies}, volume={182}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.182.4114.825}, DOI={10.1126/science.182.4114.825}, number={4114}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Olsen, Bjørn R. and Berg, Richard A. and Kishida, Yasuo and Prockop, Darwin J.}, year={1973}, month=nov, pages={825–827} }

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    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1973,
        11,
        23
      ]
    ]
  }
}