DOI: 10.1126/science.176.4036.798. Viral and Cellular DNA Polymerase: Comparison of Activities with Synthetic and Natural RNA Templates

Viral and Cellular DNA Polymerase: Comparison of Activities with Synthetic and Natural RNA Templates

10.1126/science.176.4036.798

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Two DNA polymerases purified from normal human lymphocytes are distinguishable from the viral reverse transcriptases of avian myeloblastosis virus and Mason-Pfizer monkey virus by their relative affinity for select templates. In this respect, the activity of the two normal human lymphocyte polymerases closely resembles the activity of Escherichia coli DNA polymerase 1. The viral and cellular DNA polymerases are equally active with the nonspecific template, poly(rA) ⋅ poly(dT). Criteria for distinguishing the activity of viral reverse transcriptase are discussed.

Bibliography

Robert, M. S., Smith, R. G., Gallo, R. C., Sarin, P. S., & Abrell, J. W. (1972). Viral and Cellular DNA Polymerase: Comparison of Activities with Synthetic and Natural RNA Templates. Science, 176(4036), 798â800.

Authors 5

Marjorie S. Robert (first)
R. Graham Smith (additional)
Robert C. Gallo (additional)
Prem S. Sarin (additional)
John W. Abrell (additional)

References 20 Referenced 76

10.1038/2261209a0
BALTIMORE, D, PRIMER REQUIREMENT AND TEMPLATE SPECIFICITY OF DNA POLYMERASE OF RNA TUMOR VIRUSES - (AVIAN MYELOBLASTOSIS VIRUS/MOUSE LEUKEMIA VIRUS/E-COLI DNA POLYMERASE/HOMOPOLYNUCLEOTIDES/OLIGONUCLEOTIDES), PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 68: 1507 (1971). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1971)
DUESBERG, P, COMPARATIVE PROPERTIES OF RNA AND DNA TEMPLATES FOR DNA POLYMERASE OF ROUS SARCOMA VIRUS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 68: 2505 (1971). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1971)
FRIDLENDER, B, NEW SYNTHETIC RNA-DEPENDENT DNA POLYMERASE FROM HUMAN TISSUE-CULTURE CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 69: 452 (1972). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1972)
Gallo, R. C., Biological Effects of Polynucleotides: 303 (1971). / Biological Effects of Polynucleotides (1971)
Gallo R. C. Molecular Studies in Viral Neoplasia (1974).
GALLO, R.C., RNA DEPENDENT DNA POLYMERASE OF HUMAN ACUTE LEUKAEMIC CELLS, NATURE 228: 927 (1970). (10.1038/228927a0) / NATURE (1970)
GALLO, R.C., REVERSE TRANSCRIPTASE, DNA POLYMERASE OF ONCOGENIC RNA VIRUSES, NATURE 234: 194 (1971). (10.1038/234194a0) / NATURE (1971)
GALLO, R. C., NATURE-NEW BIOLOGY 232: 140 (1971). / NATURE-NEW BIOLOGY (1971)
GOODMAN, N.C., DISTINGUISHING REVERSE TRANSCRIPTASE OF AN RNA TUMOR VIRUS FROM OTHER KNOWN DNA POLYMERASES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 68: 2203 (1971). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1971)
KACIAN, D.L., PURIFICATION OF DNA POLYMERASE OF AVIAN MYELOBLASTOSIS VIRUS, BIOCHIMICA ET BIOPHYSICA ACTA 246: 365 (1971). (10.1016/0005-2787(71)90773-8) / BIOCHIMICA ET BIOPHYSICA ACTA (1971)
10.1016/S0021-9258(19)52451-6
PENNER, P.E., RNA-DEPENDENT DNA POLYMERASE - PRESENCE IN NORMAL HUMAN CELLS, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 42: 1228 (1971). (10.1016/0006-291X(71)90037-4) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1971)
PENNER, P. E., NATURE-NEW BIOLOGY 232: 58 (1971). / NATURE-NEW BIOLOGY (1971)
Sarin P. S. in preparation.
SCOLNICK, E.M., RNA DEPENDENT DNA POLYMERASE ACTIVITY IN MAMMALIAN CELLS, NATURE 229: 318 (1971). (10.1038/229318a0) / NATURE (1971)
Smith R. G. in preparation.
SPIEGELMAN, S, SYNTHETIC DNA-RNA HYBRIDS AND RNA-RNA DUPLEXES AS TEMPLATES FOR POLYMERASES OF ONCOGENIC RNA VIRUSES, NATURE 228: 430 (1970). (10.1038/228430a0) / NATURE (1970)
10.1038/2261211a0
Wells, R. D., Biochemistry 11: 621 (1972). (10.1021/bi00754a025) / Biochemistry (1972)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 10:03 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 11:09 a.m.)
Indexed	1 year, 7 months ago (Jan. 11, 2024, 7:03 p.m.)
Issued	53 years, 3 months ago (May 19, 1972)
Published	53 years, 3 months ago (May 19, 1972)
Published Print	53 years, 3 months ago (May 19, 1972)

Funders 0

None

BibTeX

@article{Robert_1972, title={Viral and Cellular DNA Polymerase: Comparison of Activities with Synthetic and Natural RNA Templates}, volume={176}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.176.4036.798}, DOI={10.1126/science.176.4036.798}, number={4036}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Robert, Marjorie S. and Smith, R. Graham and Gallo, Robert C. and Sarin, Prem S. and Abrell, John W.}, year={1972}, month=may, pages={798–800} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        1,
        12
      ]
    ],
    "date-time": "2024-01-12T00:03:51Z",
    "timestamp": 1705017831412
  },
  "reference-count": 20,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "4036",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1972,
        5,
        19
      ]
    ]
  },
  "abstract": "<jats:p>\n            Two DNA polymerases purified from normal human lymphocytes are distinguishable from the viral reverse transcriptases of avian myeloblastosis virus and Mason-Pfizer monkey virus by their relative affinity for select templates. In this respect, the activity of the two normal human lymphocyte polymerases closely resembles the activity of\n            <jats:italic>Escherichia coli</jats:italic>\n            DNA polymerase 1. The viral and cellular DNA polymerases are equally active with the nonspecific template, poly(rA) \u22c5 poly(dT). Criteria for distinguishing the activity of viral reverse transcriptase are discussed.\n          </jats:p>",
  "DOI": "10.1126/science.176.4036.798",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        10,
        5
      ]
    ],
    "date-time": "2006-10-05T14:03:38Z",
    "timestamp": 1160057018000
  },
  "page": "798-800",
  "source": "Crossref",
  "is-referenced-by-count": 76,
  "title": "Viral and Cellular DNA Polymerase: Comparison of Activities with Synthetic and Natural RNA Templates",
  "prefix": "10.1126",
  "volume": "176",
  "author": [
    {
      "given": "Marjorie S.",
      "family": "Robert",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Maryland 20014"
        }
      ]
    },
    {
      "given": "R. Graham",
      "family": "Smith",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Maryland 20014"
        }
      ]
    },
    {
      "given": "Robert C.",
      "family": "Gallo",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Maryland 20014"
        }
      ]
    },
    {
      "given": "Prem S.",
      "family": "Sarin",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Bionetics Research Laboratories, Bethesda, Maryland 20014"
        }
      ]
    },
    {
      "given": "John W.",
      "family": "Abrell",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Bionetics Research Laboratories, Bethesda, Maryland 20014"
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_2_1_1_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/2261209a0"
    },
    {
      "key": "e_1_2_1_2_1",
      "first-page": "1507",
      "volume": "68",
      "year": "1971",
      "unstructured": "BALTIMORE, D, PRIMER REQUIREMENT AND TEMPLATE SPECIFICITY OF DNA POLYMERASE OF RNA TUMOR VIRUSES - (AVIAN MYELOBLASTOSIS VIRUS/MOUSE LEUKEMIA VIRUS/E-COLI DNA POLYMERASE/HOMOPOLYNUCLEOTIDES/OLIGONUCLEOTIDES), PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 68: 1507 (1971).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_3_1",
      "first-page": "2505",
      "volume": "68",
      "year": "1971",
      "unstructured": "DUESBERG, P, COMPARATIVE PROPERTIES OF RNA AND DNA TEMPLATES FOR DNA POLYMERASE OF ROUS SARCOMA VIRUS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 68: 2505 (1971).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_4_1",
      "first-page": "452",
      "volume": "69",
      "year": "1972",
      "unstructured": "FRIDLENDER, B, NEW SYNTHETIC RNA-DEPENDENT DNA POLYMERASE FROM HUMAN TISSUE-CULTURE CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 69: 452 (1972).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_5_1",
      "first-page": "303",
      "year": "1971",
      "unstructured": "Gallo, R. C., Biological Effects of Polynucleotides: 303 (1971).",
      "journal-title": "Biological Effects of Polynucleotides"
    },
    {
      "key": "e_1_2_1_6_1",
      "unstructured": "Gallo R. C. Molecular Studies in Viral Neoplasia (1974)."
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "crossref",
      "first-page": "927",
      "DOI": "10.1038/228927a0",
      "volume": "228",
      "year": "1970",
      "unstructured": "GALLO, R.C., RNA DEPENDENT DNA POLYMERASE OF HUMAN ACUTE LEUKAEMIC CELLS, NATURE 228: 927 (1970).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "crossref",
      "first-page": "194",
      "DOI": "10.1038/234194a0",
      "volume": "234",
      "year": "1971",
      "unstructured": "GALLO, R.C., REVERSE TRANSCRIPTASE, DNA POLYMERASE OF ONCOGENIC RNA VIRUSES, NATURE 234: 194 (1971).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_2_1_9_1",
      "first-page": "140",
      "volume": "232",
      "year": "1971",
      "unstructured": "GALLO, R. C., NATURE-NEW BIOLOGY 232: 140 (1971).",
      "journal-title": "NATURE-NEW BIOLOGY"
    },
    {
      "key": "e_1_2_1_10_1",
      "first-page": "2203",
      "volume": "68",
      "year": "1971",
      "unstructured": "GOODMAN, N.C., DISTINGUISHING REVERSE TRANSCRIPTASE OF AN RNA TUMOR VIRUS FROM OTHER KNOWN DNA POLYMERASES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 68: 2203 (1971).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "crossref",
      "first-page": "365",
      "DOI": "10.1016/0005-2787(71)90773-8",
      "volume": "246",
      "year": "1971",
      "unstructured": "KACIAN, D.L., PURIFICATION OF DNA POLYMERASE OF AVIAN MYELOBLASTOSIS VIRUS, BIOCHIMICA ET BIOPHYSICA ACTA 246: 365 (1971).",
      "journal-title": "BIOCHIMICA ET BIOPHYSICA ACTA"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)52451-6"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "crossref",
      "first-page": "1228",
      "DOI": "10.1016/0006-291X(71)90037-4",
      "volume": "42",
      "year": "1971",
      "unstructured": "PENNER, P.E., RNA-DEPENDENT DNA POLYMERASE - PRESENCE IN NORMAL HUMAN CELLS, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 42: 1228 (1971).",
      "journal-title": "BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS"
    },
    {
      "key": "e_1_2_1_14_1",
      "first-page": "58",
      "volume": "232",
      "year": "1971",
      "unstructured": "PENNER, P. E., NATURE-NEW BIOLOGY 232: 58 (1971).",
      "journal-title": "NATURE-NEW BIOLOGY"
    },
    {
      "key": "e_1_2_1_15_1",
      "unstructured": "Sarin P. S. in preparation."
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "crossref",
      "first-page": "318",
      "DOI": "10.1038/229318a0",
      "volume": "229",
      "year": "1971",
      "unstructured": "SCOLNICK, E.M., RNA DEPENDENT DNA POLYMERASE ACTIVITY IN MAMMALIAN CELLS, NATURE 229: 318 (1971).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_2_1_17_1",
      "unstructured": "Smith R. G. in preparation."
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "crossref",
      "first-page": "430",
      "DOI": "10.1038/228430a0",
      "volume": "228",
      "year": "1970",
      "unstructured": "SPIEGELMAN, S, SYNTHETIC DNA-RNA HYBRIDS AND RNA-RNA DUPLEXES AS TEMPLATES FOR POLYMERASES OF ONCOGENIC RNA VIRUSES, NATURE 228: 430 (1970).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/2261211a0"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "crossref",
      "first-page": "621",
      "DOI": "10.1021/bi00754a025",
      "volume": "11",
      "year": "1972",
      "unstructured": "Wells, R. D., Biochemistry 11: 621 (1972).",
      "journal-title": "Biochemistry"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.176.4036.798",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        11
      ]
    ],
    "date-time": "2024-01-11T16:09:40Z",
    "timestamp": 1704989380000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.176.4036.798"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1972,
        5,
        19
      ]
    ]
  },
  "references-count": 20,
  "journal-issue": {
    "issue": "4036",
    "published-print": {
      "date-parts": [
        [
          1972,
          5,
          19
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.176.4036.798"
  ],
  "URL": "http://dx.doi.org/10.1126/science.176.4036.798",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1972,
        5,
        19
      ]
    ]
  }
}