DOI: 10.1126/science.176.4033.412. Branching of Central Neurons: Intracellular Cobalt Injection for Light and Electron Microscopy

Branching of Central Neurons: Intracellular Cobalt Injection for Light and Electron Microscopy

10.1126/science.176.4033.412

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Cobalt chloride can be injected into an identified nerve cell body in an insect ganglion and reacted with ammonium sulfide to stain the soma and its branches with a black precipitate. The stained cell body and its branches throughout the neuropil are visible in both the light and electron microscope. In whole mount preparations, the resolution of neurites within the neuropil is of a quality that permits the comparison of branching patterns between cells and during various functional states.

Bibliography

Pitman, R. M., Tweedle, C. D., & Cohen, M. J. (1972). Branching of Central Neurons: Intracellular Cobalt Injection for Light and Electron Microscopy. Science, 176(4033), 412â414.

Authors 3

Robert M. Pitman (first)
Charles D. Tweedle (additional)
Melvin J. Cohen (additional)

References 11 Referenced 399

BLACKSTAD, T.W., CONT RES METHODS NEU: 186 (1970). / CONT RES METHODS NEU (1970)
CARBONELL, C.S., SMITHSONIAN MISCELLANEOUS COLLECTIONS 107: 1 (1947). / SMITHSONIAN MISCELLANEOUS COLLECTIONS (1947)
COHEN, M.J., FUNCTIONAL ORGANIZATION OF MOTOR NEURONS IN AN INSECT GANGLION, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES 252: 561 (1967). / PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES (1967)
KARNOVSKY, M.J., A FORMALDEHYDE-GLUTARALDEHYDE FIXATIVE OF HIGH OSMOLALITY FOR USE IN ELECTRON MICROSCOPY, JOURNAL OF CELL BIOLOGY 27: A137 (1965). / JOURNAL OF CELL BIOLOGY (1965)
KERKUT, G.A., IONTOPHORETIC APPLICATION OF ACETYLCHOLINE AND GABA ONTO INSECT CENTRAL NEURONES, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY 31: 611 (1969). (10.1016/0010-406X(69)90063-2) / COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY (1969)
10.1126/science.166.3913.1641
10.1126/science.162.3850.281
SPURR, A.R., A LOW-VISCOSITY EPOXY RESIN EMBEDDING MEDIUM FOR ELECTRON MICROSCOPY, JOURNAL OF ULTRASTRUCTURE RESEARCH 26: 31 (1969). (10.1016/S0022-5320(69)90033-1) / JOURNAL OF ULTRASTRUCTURE RESEARCH (1969)
Stretton, A. O. W., Science 162:134 (1968). / Science (1968)
TRUJILLOCENOZ, O, LIGHT AND ELECTRONMICROSCOPE STUDY OF ONE OF SYSTEMS OF CENTRIFUGAL FIBERS FOUND IN LAMINA OF MUSCOID FLIES, ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE 110: 336 (1970). (10.1007/BF00321146) / ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE (1970)
USHERWOOD, PNR, INSECT NEUROMUSCULAR MECHANISMS, AMERICAN ZOOLOGIST 7: 553 (1967). (10.1093/icb/7.3.553) / AMERICAN ZOOLOGIST (1967)

Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 10:03 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 10:26 a.m.)
Indexed	1 year ago (Aug. 17, 2024, 3:11 a.m.)
Issued	53 years, 4 months ago (April 28, 1972)
Published	53 years, 4 months ago (April 28, 1972)
Published Print	53 years, 4 months ago (April 28, 1972)

Funders 0

None

BibTeX

@article{Pitman_1972, title={Branching of Central Neurons: Intracellular Cobalt Injection for Light and Electron Microscopy}, volume={176}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.176.4033.412}, DOI={10.1126/science.176.4033.412}, number={4033}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Pitman, Robert M. and Tweedle, Charles D. and Cohen, Melvin J.}, year={1972}, month=apr, pages={412–414} }

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