DOI: 10.1126/science.175.4027.1261. Activities of Sodium and Potassium Ions in Epithelial Cells of Small Intestine

Activities of Sodium and Potassium Ions in Epithelial Cells of Small Intestine

10.1126/science.175.4027.1261

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Intracellular molar Na + activity ( a Na ), measured with cation-selective glass microelectrodes, in epithelial cells of isolated bullfrog small intestine immersed at 26°C in a sodium sulfate Ringer solution containing mannitol was 0.0144 ± 0.0031 (average value plus or minus standard deviation). The corresponding K + activity ( a K ) was 0.0854 ± 0.0060. Combination of these values with previous estimates of intracellular Na + and K + concentrations under identical conditions indicated that a substantial fraction of the cellular Na + is in an osmotically inactive state. When the cells were exposed to a Ringer solution in which 26 millimoles of mannitol per liter were replaced by 3- O -methyl glucose, highly significant decreases in a K and a Na were observed. The decrease in a K was proportionately larger than the corresponding decrease in a Na .

Bibliography

Lee, C. O., & Armstrong, W. McD. (1972). Activities of Sodium and Potassium Ions in Epithelial Cells of Small Intestine. Science, 175(4027), 1261â1264.

Authors 2

Chin O. Lee (first)
W. McD. Armstrong (additional)

References 24 Referenced 81

ARMSTRONG, W.M., SODIUM, POTASSIUM, AND WATER CONTENT OF ISOLATED BULLFROG SMALL INTESTINAL EPITHELIA, AMERICAN JOURNAL OF PHYSIOLOGY 219: 1023 (1970). (10.1152/ajplegacy.1970.219.4.1023) / AMERICAN JOURNAL OF PHYSIOLOGY (1970)
10.1126/science.171.3969.413
BAKER, R.D., INTESTINAL TRANSMURAL ELECTRICAL ACTIVITY - SELECTIVE EFFECTS OF MUCOSAL AND SEROSAL ANAEROBIOSIS, BIOCHIMICA ET BIOPHYSICA ACTA 225: 392 (1971). (10.1016/0005-2736(71)90237-9) / BIOCHIMICA ET BIOPHYSICA ACTA (1971)
CRANE, R.K., HYPOTHESIS FOR MECHANISM OF INTESTINAL ACTIVE TRANSPORT OF SUGARS, FEDERATION PROCEEDINGS 21: 891 (1962). / FEDERATION PROCEEDINGS (1962)
CRANE, R.K., FEDERATION PROCEEDINGS 24: 1000 (1965). / FEDERATION PROCEEDINGS (1965)
CSAKY, T.Z., OSMOTIC SWELLING OF INTESTINAL EPITHELIAL CELLS DURING ACTIVE SUGAR TRANSPORT, AMERICAN JOURNAL OF PHYSIOLOGY 217: 753 (1969). (10.1152/ajplegacy.1969.217.3.753) / AMERICAN JOURNAL OF PHYSIOLOGY (1969)
10.1126/science.126.3278.831
FORDTRAN, J.S., MECHANISMS OF SODIUM ABSORPTION IN HUMAN SMALL INTESTINE, JOURNAL OF CLINICAL INVESTIGATION 47: 884 (1968). (10.1172/JCI105781) / JOURNAL OF CLINICAL INVESTIGATION (1968)
FREEMAN, J.R., DIELECTRIC PROPERTIES OF MINERALIZED TISSUES, TRANSACTIONS OF THE NEW YORK ACADEMY OF SCIENCES 29: 623 (1967). (10.1111/j.2164-0947.1967.tb02432.x) / TRANSACTIONS OF THE NEW YORK ACADEMY OF SCIENCES (1967)
HINKE, J. A. M., CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY 45: 655 (1967). (10.1139/y67-078) / CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (1967)
Hinke J. A. M. Glass Microelectrodes (1969).
HINKE, J. A. M., SOLVENT WATER FOR ELECTROLYTES IN MUSCLE FIBER OF GIANT BARNACLE, JOURNAL OF GENERAL PHYSIOLOGY 56: 521 (1970). (10.1085/jgp.56.4.521) / JOURNAL OF GENERAL PHYSIOLOGY (1970)
KIMMICH, G.A., ACTIVE SUGAR ACCUMULATION BY ISOLATED INTESTINAL EPITHELIAL CELLS - A NEW MODEL FOR SODIUM-DEPENDENT METABOLITE TRANSPORT, BIOCHEMISTRY 9: 3669 (1970). (10.1021/bi00821a004) / BIOCHEMISTRY (1970)
KOOPMAN, W, EFFECT OF SUGARS AND AMINO ACIDS ON MUCOSAL NA+ AND K+ CONCENTRATIONS IN RABBIT ILEUM, BIOCHIMICA ET BIOPHYSICA ACTA 173: 338 (1969). (10.1016/0005-2736(69)90116-3) / BIOCHIMICA ET BIOPHYSICA ACTA (1969)
KRYNEVIC, K, NATURE 228: 119 (1970). (10.1038/228119a0) / NATURE (1970)
LEV, A.A., DETERMINATION OF ACTIVITY + ACTIVITY COEFFICIENTS OF POTASSIUM + SODIUM IONS IN FROG MUSCLE FIBRES, NATURE 201: 1132 (1964). (10.1038/2011132a0) / NATURE (1964)
LIM, J.J., INVESTIGATION OF BOUND WATER IN TENDON BY DIELECTRIC MEASUREMENT, BIOPHYSICAL JOURNAL 11: 648 (1971). (10.1016/S0006-3495(71)86244-6) / BIOPHYSICAL JOURNAL (1971)
MCLAUGHLIN, SGH, SODIUM AND WATER BINDING IN SINGLE STRIATED MUSCLE FIBERS OF GIANT BARNACLE, CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY 44: 837 (1966). (10.1139/y66-102) / CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (1966)
NICOLSKY, B.P., ZHURNAL FIZICHESKOI KHIMII 10: 495 (1937). / ZHURNAL FIZICHESKOI KHIMII (1937)
QUAY, J.F., SODIUM AND CHLORIDE TRANSPORT BY ISOLATED BULLFROG SMALL INTESTINE, AMERICAN JOURNAL OF PHYSIOLOGY 217: 694 (1969). (10.1152/ajplegacy.1969.217.3.694) / AMERICAN JOURNAL OF PHYSIOLOGY (1969)
QUAY, J.F., ENHANCEMENT OF NET SODIUM TRANSPORT IN ISOLATED BULLFROG INTESTINE BY SUGARS AND AMINO ACIDS, PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE 131: 46 (1969). (10.3181/00379727-131-33801) / PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE (1969)
Robinson R. A. Electrolyte Solutions (1965).
SCHULTZ, S.G., COUPLED TRANSPORT OF SODIUM AND ORGANIC SOLUTES, PHYSIOLOGICAL REVIEWS 50: 637 (1970). (10.1152/physrev.1970.50.4.637) / PHYSIOLOGICAL REVIEWS (1970)
WHITE, J.F., EFFECT OF TRANSPORTED SOLUTES ON MEMBRANE POTENTIALS IN BULLFROG SMALL INTESTINE, AMERICAN JOURNAL OF PHYSIOLOGY 221: 194 (1971). (10.1152/ajplegacy.1971.221.1.194) / AMERICAN JOURNAL OF PHYSIOLOGY (1971)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 10:03 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 10:11 a.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 1:19 p.m.)
Issued	53 years, 5 months ago (March 17, 1972)
Published	53 years, 5 months ago (March 17, 1972)
Published Print	53 years, 5 months ago (March 17, 1972)

Funders 0

None

BibTeX

@article{Lee_1972, title={Activities of Sodium and Potassium Ions in Epithelial Cells of Small Intestine}, volume={175}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.175.4027.1261}, DOI={10.1126/science.175.4027.1261}, number={4027}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Lee, Chin O. and Armstrong, W. McD.}, year={1972}, month=mar, pages={1261–1264} }

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