DOI: 10.1126/science.174.4008.500. Buffer Combinations for Mammalian Cell Culture

Buffer Combinations for Mammalian Cell Culture

10.1126/science.174.4008.500

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The growth and metabolism of cultured mammalian cells are markedly affected by the p H variation in ordinary bicarbonate-buffered media ( p H 8.0 to 6.9). Those p H swings can be reduced and the p H of the culture can be stabilized as desired in the range p H 6.4 to 8.3 by appropriate combinations of two or three organic buffers, each at 10 to 15 millimolar, in conjunction with phosphate and bicarbonate. The initial alkalinization in sparse cultures is then minimized, and the metabolic acidification in 24 hours is usually less than 0.4 p H unit except in heavy cultures.

Bibliography

Eagle, H. (1971). Buffer Combinations for Mammalian Cell Culture. Science, 174(4008), 500â503.

Authors 1

Harry Eagle (first)

References 14 Referenced 233

CECCARINI, C, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 68: 299 (1971). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1971)
10.1016/0003-9861(56)90358-7
Eagle H. Nature in press.
EAGLE, H, GROWTH CHARACTERISTICS OF VIRUS-TRANSFORMED CELLS - MAXIMUM POPULATION DENSITY, INHIBITION BY NORMAL CELLS, SERUM REQUIREMENT, GROWTH IN SOFT AGAR, AND XENOGENEIC TRANSPLANTABILITY, JOURNAL OF EXPERIMENTAL MEDICINE 131: 863 (1970). (10.1084/jem.131.4.863) / JOURNAL OF EXPERIMENTAL MEDICINE (1970)
EAGLE, H, GROWTH REGULATORY EFFECTS OF CELLULAR INTERACTION, NATURE 213: 1102 (1967). (10.1038/2131102a0) / NATURE (1967)
EAGLE, H, SPECIES SPECIFICITY IN GROWTH REGULATORY EFFECTS OF CELLULAR INTERACTION, NATURE 220: 266 (1968). (10.1038/220266a0) / NATURE (1968)
10.1126/science.130.3373.432
GEYER, R.P., BICARBONATE AS AN ESSENTIAL FOR HUMAN CELLS INVITRO, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 73: 500 (1958). (10.1016/0003-9861(58)90293-5) / ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (1958)
10.1021/bi00866a011
LEVINE, E.M., CONTACT INHIBITION MACROMOLECULAR SYNTHESIS AND POLYRIBOSOMES IN CULTURED HUMAN DIPLOID FIBROBLASTS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 53: 350 (1965). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1965)
MACKENZIE, C.G., EFFECT OF PH ON GROWTH, PROTEIN SYNTHESIS, AND LIPID-RICH PARTICLES OF CULTURED MAMMALIAN CELLS, JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY 9: 141 (1961). (10.1083/jcb.9.1.141) / JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY (1961)
OYAMA, V.I., MEASUREMENT OF CELL GROWTH IN TISSUE CULTURE WITH A PHENOL REAGENT (FOLIN-CIOCALTEAU), PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE 91: 305 (1956). (10.3181/00379727-91-22245) / PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE (1956)
SWIM, H.E., THE ROLE OF CARBON DIOXIDE AS AN ESSENTIAL NUTRIENT FOR 6 PERMANENT STRAINS OF FIBROBLASTS, JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY 4: 525 (1958). (10.1083/jcb.4.5.525) / JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY (1958)
TAYLOR, A.C., RESPONSES OF CELLS TO PH CHANGES IN MEDIUM, JOURNAL OF CELL BIOLOGY 15: 201 (1962). (10.1083/jcb.15.2.201) / JOURNAL OF CELL BIOLOGY (1962)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 9:17 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 10:14 a.m.)
Indexed	1 year, 1 month ago (July 4, 2024, 12:44 a.m.)
Issued	53 years, 10 months ago (Oct. 29, 1971)
Published	53 years, 10 months ago (Oct. 29, 1971)
Published Print	53 years, 10 months ago (Oct. 29, 1971)

Funders 0

None

BibTeX

@article{Eagle_1971, title={Buffer Combinations for Mammalian Cell Culture}, volume={174}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.174.4008.500}, DOI={10.1126/science.174.4008.500}, number={4008}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Eagle, Harry}, year={1971}, month=oct, pages={500–503} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        7,
        4
      ]
    ],
    "date-time": "2024-07-04T04:44:24Z",
    "timestamp": 1720068264897
  },
  "reference-count": 14,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "4008",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1971,
        10,
        29
      ]
    ]
  },
  "abstract": "<jats:p>\n            The growth and metabolism of cultured mammalian cells are markedly affected by the\n            <jats:italic>p</jats:italic>\n            H variation in ordinary bicarbonate-buffered media (\n            <jats:italic>p</jats:italic>\n            H 8.0 to 6.9). Those\n            <jats:italic>p</jats:italic>\n            H swings can be reduced and the\n            <jats:italic>p</jats:italic>\n            H of the culture can be stabilized as desired in the range\n            <jats:italic>p</jats:italic>\n            H 6.4 to 8.3 by appropriate combinations of two or three organic buffers, each at 10 to 15 millimolar, in conjunction with phosphate and bicarbonate. The initial alkalinization in sparse cultures is then minimized, and the metabolic acidification in 24 hours is usually less than 0.4\n            <jats:italic>p</jats:italic>\n            H unit except in heavy cultures.\n          </jats:p>",
  "DOI": "10.1126/science.174.4008.500",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        10,
        5
      ]
    ],
    "date-time": "2006-10-05T13:17:43Z",
    "timestamp": 1160054263000
  },
  "page": "500-503",
  "source": "Crossref",
  "is-referenced-by-count": 233,
  "title": "Buffer Combinations for Mammalian Cell Culture",
  "prefix": "10.1126",
  "volume": "174",
  "author": [
    {
      "given": "Harry",
      "family": "Eagle",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Albert Einstein College of Medicine, Bronx, New York 10461"
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_2_1_1_1",
      "first-page": "299",
      "volume": "68",
      "year": "1971",
      "unstructured": "CECCARINI, C, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 68: 299 (1971).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-9861(56)90358-7"
    },
    {
      "key": "e_1_2_1_3_1",
      "unstructured": "Eagle H. Nature in press."
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "crossref",
      "first-page": "863",
      "DOI": "10.1084/jem.131.4.863",
      "volume": "131",
      "year": "1970",
      "unstructured": "EAGLE, H, GROWTH CHARACTERISTICS OF VIRUS-TRANSFORMED CELLS - MAXIMUM POPULATION DENSITY, INHIBITION BY NORMAL CELLS, SERUM REQUIREMENT, GROWTH IN SOFT AGAR, AND XENOGENEIC TRANSPLANTABILITY, JOURNAL OF EXPERIMENTAL MEDICINE 131: 863 (1970).",
      "journal-title": "JOURNAL OF EXPERIMENTAL MEDICINE"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "crossref",
      "first-page": "1102",
      "DOI": "10.1038/2131102a0",
      "volume": "213",
      "year": "1967",
      "unstructured": "EAGLE, H, GROWTH REGULATORY EFFECTS OF CELLULAR INTERACTION, NATURE 213: 1102 (1967).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "crossref",
      "first-page": "266",
      "DOI": "10.1038/220266a0",
      "volume": "220",
      "year": "1968",
      "unstructured": "EAGLE, H, SPECIES SPECIFICITY IN GROWTH REGULATORY EFFECTS OF CELLULAR INTERACTION, NATURE 220: 266 (1968).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.130.3373.432"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "crossref",
      "first-page": "500",
      "DOI": "10.1016/0003-9861(58)90293-5",
      "volume": "73",
      "year": "1958",
      "unstructured": "GEYER, R.P., BICARBONATE AS AN ESSENTIAL FOR HUMAN CELLS INVITRO, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 73: 500 (1958).",
      "journal-title": "ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00866a011"
    },
    {
      "key": "e_1_2_1_10_1",
      "first-page": "350",
      "volume": "53",
      "year": "1965",
      "unstructured": "LEVINE, E.M., CONTACT INHIBITION MACROMOLECULAR SYNTHESIS AND POLYRIBOSOMES IN CULTURED HUMAN DIPLOID FIBROBLASTS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 53: 350 (1965).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "crossref",
      "first-page": "141",
      "DOI": "10.1083/jcb.9.1.141",
      "volume": "9",
      "year": "1961",
      "unstructured": "MACKENZIE, C.G., EFFECT OF PH ON GROWTH, PROTEIN SYNTHESIS, AND LIPID-RICH PARTICLES OF CULTURED MAMMALIAN CELLS, JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY 9: 141 (1961).",
      "journal-title": "JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "crossref",
      "first-page": "305",
      "DOI": "10.3181/00379727-91-22245",
      "volume": "91",
      "year": "1956",
      "unstructured": "OYAMA, V.I., MEASUREMENT OF CELL GROWTH IN TISSUE CULTURE WITH A PHENOL REAGENT (FOLIN-CIOCALTEAU), PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE 91: 305 (1956).",
      "journal-title": "PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "crossref",
      "first-page": "525",
      "DOI": "10.1083/jcb.4.5.525",
      "volume": "4",
      "year": "1958",
      "unstructured": "SWIM, H.E., THE ROLE OF CARBON DIOXIDE AS AN ESSENTIAL NUTRIENT FOR 6 PERMANENT STRAINS OF FIBROBLASTS, JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY 4: 525 (1958).",
      "journal-title": "JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "crossref",
      "first-page": "201",
      "DOI": "10.1083/jcb.15.2.201",
      "volume": "15",
      "year": "1962",
      "unstructured": "TAYLOR, A.C., RESPONSES OF CELLS TO PH CHANGES IN MEDIUM, JOURNAL OF CELL BIOLOGY 15: 201 (1962).",
      "journal-title": "JOURNAL OF CELL BIOLOGY"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.174.4008.500",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        11
      ]
    ],
    "date-time": "2024-01-11T15:14:08Z",
    "timestamp": 1704986048000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.174.4008.500"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1971,
        10,
        29
      ]
    ]
  },
  "references-count": 14,
  "journal-issue": {
    "issue": "4008",
    "published-print": {
      "date-parts": [
        [
          1971,
          10,
          29
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.174.4008.500"
  ],
  "URL": "http://dx.doi.org/10.1126/science.174.4008.500",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1971,
        10,
        29
      ]
    ]
  }
}