DOI: 10.1126/science.1700866. Increase of the Catalytic Activity of Phospholipase C-γ1 by Tyrosine Phosphorylation

Increase of the Catalytic Activity of Phospholipase C-γ1 by Tyrosine Phosphorylation

10.1126/science.1700866

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Phospholipase C-γ1 (PLC-γ1), an isozyme of the phosphoinositide-specific phospholipase C family, which occupies a central role in hormonal signal transduction pathways, is an excellent substrate for the epidermal growth factor (EGF) receptor tyrosine kinase. Epidermal growth factor elicits tyrosine phosphorylation of PLC-γ1 and phosphatidylinositol 4,5-bisphosphate hydrolysis in various cell lines. The ability of tyrosine phosphorylation to activate the catalytic activity of PLC-γ1 was tested. Tyrosine phosphorylation in intact cells or in vitro increased the catalytic activity of PLC-γ1. Also, treatment of EGF-activated PLC-γ1 with a tyrosine-specific phosphatase substantially decreased the catalytic activity of PLC-γ1. These results suggest that the EGF-stimulated formation of inositol 1,4,5-trisphosphate and diacylglycerol in intact cells results, at least in part, from catalytic activation of PLC-γ1 through tyrosine phosphorylation.

Bibliography

Nishibe, S., Wahl, M. I., Hernandez-Sotomayor, S. M., Tonks, N. K., Rhee, S. G., & Carpenter, G. (1990). Increase of the Catalytic Activity of Phospholipase C-Î³1 by Tyrosine Phosphorylation. Science, 250(4985), 1253â1256.

Authors 6

Shunzo Nishibe (first)
Matthew I. Wahl (additional)
S. M. Hernandez-Sotomayor (additional)
Nicholas K. Tonks (additional)
Sue Goo Rhee (additional)
Graham Carpenter (additional)

References 25 Referenced 499

10.1038/343651a0
10.1038/341197a0
CHEN, W.S., REQUIREMENT FOR INTRINSIC PROTEIN TYROSINE KINASE IN THE IMMEDIATE AND LATE ACTIONS OF THE EGF RECEPTOR, NATURE 328: 820 (1987). (10.1038/328820a0) / NATURE (1987)
COCKCROFT, S, POLYPHOSPHOINOSITIDE PHOSPHODIESTERASE - REGULATION BY A NOVEL GUANINE-NUCLEOTIDE BINDING PROTEIN-GP, TRENDS IN BIOCHEMICAL SCIENCES 12: 75 (1987). (10.1016/0968-0004(87)90035-1) / TRENDS IN BIOCHEMICAL SCIENCES (1987)
COOL, D.E., CDNA ISOLATED FROM A HUMAN T-CELL LIBRARY ENCODES A MEMBER OF THE PROTEIN-TYROSINE-PHOSPHATASE FAMILY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 5257 (1989). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1989)
COOL, D.E., EXPRESSION OF A HUMAN T-CELL PROTEIN-TYROSINE-PHOSPHATASE IN BABY HAMSTER-KIDNEY CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 87: 7280 (1990). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1990)
HERNANDEZSOTOMA.SM unpublished data.
HUNTER, T, CURR OPIN CELL BIOL 1: 1168 (1989). (10.1016/S0955-0674(89)80068-7) / CURR OPIN CELL BIOL (1989)
KAMPS, M.P., ACID AND BASE HYDROLYSIS OF PHOSPHOPROTEINS BOUND TO IMMOBILON FACILITATES ANALYSIS OF PHOSPHOAMINO ACIDS IN GEL-FRACTIONATED PROTEINS, ANALYTICAL BIOCHEMISTRY 176: 22 (1989). (10.1016/0003-2697(89)90266-2) / ANALYTICAL BIOCHEMISTRY (1989)
10.1016/0092-8674(89)90047-0
10.1126/science.2333512
10.1016/0092-8674(89)90048-2
MOOLENAAR, W.H., A POINT MUTATION AT THE ATP-BINDING SITE OF THE EGF-RECEPTOR ABOLISHES SIGNAL TRANSDUCTION, EMBO JOURNAL 7: 707 (1988). (10.1002/j.1460-2075.1988.tb02866.x) / EMBO JOURNAL (1988)
NISHIBE, S, TYROSINE PHOSPHORYLATION OF PHOSPHOLIPASE-C-II INVITRO BY THE EPIDERMAL GROWTH-FACTOR RECEPTOR, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 10335 (1989). (10.1016/S0021-9258(18)81622-2) / JOURNAL OF BIOLOGICAL CHEMISTRY (1989)
NISHIBE S unpublished data.
10.1038/334661a0
RYU, S.H., PURIFICATION AND CHARACTERIZATION OF 2 IMMUNOLOGICALLY DISTINCT PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C FROM BOVINE BRAIN, JOURNAL OF BIOLOGICAL CHEMISTRY 262: 12511 (1987). (10.1016/S0021-9258(18)45235-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1987)
SUH, P.G., MONOCLONAL-ANTIBODIES TO 3 PHOSPHOLIPASE-C ISOZYMES FROM BOVINE BRAIN, JOURNAL OF BIOLOGICAL CHEMISTRY 263: 14497 (1988). (10.1016/S0021-9258(18)68247-X) / JOURNAL OF BIOLOGICAL CHEMISTRY (1988)
10.1126/science.2374928
TONKS, N.K., BIOCHEMISTRY-US 27: 8695 (1988). (10.1021/bi00424a001) / BIOCHEMISTRY-US (1988)
WAHL, M.I., EPIDERMAL GROWTH-FACTOR (EGF) STIMULATES INOSITOL TRISPHOSPHATE FORMATION IN CELLS WHICH OVEREXPRESS THE EGF RECEPTOR, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 142: 688 (1987). (10.1016/0006-291X(87)91469-0) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1987)
WAHL, M.I., GROWTH-FACTOR SIGNALING PATHWAYS - PHOSPHOINOSITIDE METABOLISM AND PHOSPHORYLATION OF PHOSPHOLIPASE-C, CANCER CELLS-A MONTHLY REVIEW 1: 101 (1989). / CANCER CELLS-A MONTHLY REVIEW (1989)
WAHL, M.I., IDENTIFICATION OF 2 EPIDERMAL GROWTH FACTOR-SENSITIVE TYROSINE PHOSPHORYLATION SITES OF PHOSPHOLIPASE C-GAMMA IN INTACT HSC-1 CELLS, JOURNAL OF BIOLOGICAL CHEMISTRY 265: 3944 (1990). (10.1016/S0021-9258(19)39685-1) / JOURNAL OF BIOLOGICAL CHEMISTRY (1990)
WAHL, M.I., EPIDERMAL GROWTH-FACTOR STIMULATES TYROSINE PHOSPHORYLATION OF PHOSPHOLIPASE C-II INDEPENDENTLY OF RECEPTOR INTERNALIZATION AND EXTRACELLULAR CALCIUM, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 1568 (1989). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1989)
10.1126/science.2457254

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 9:41 a.m.)
Indexed	4 months, 2 weeks ago (April 5, 2025, 5:07 a.m.)
Issued	34 years, 8 months ago (Nov. 30, 1990)
Published	34 years, 8 months ago (Nov. 30, 1990)
Published Print	34 years, 8 months ago (Nov. 30, 1990)

Funders 0

None

BibTeX

@article{Nishibe_1990, title={Increase of the Catalytic Activity of Phospholipase C-γ1 by Tyrosine Phosphorylation}, volume={250}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1700866}, DOI={10.1126/science.1700866}, number={4985}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Nishibe, Shunzo and Wahl, Matthew I. and Hernandez-Sotomayor, S. M. and Tonks, Nicholas K. and Rhee, Sue Goo and Carpenter, Graham}, year={1990}, month=nov, pages={1253–1256} }

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