DOI: 10.1126/science.169.3948.880. Desmosterol as the Major Sterol in L-Cell Mouse Fibroblasts Grown in Sterol-Free Culture Medium

Desmosterol as the Major Sterol in L-Cell Mouse Fibroblasts Grown in Sterol-Free Culture Medium

10.1126/science.169.3948.880

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The principal sterol synthesized by L-cell mouse fibroblasts is desmosterol. Cholesterol was not detected in these cells when they were grown in a sterol-free culture medium. These findings indicate that, in cells, cholesterol can be replaced by desmosterol. Sterol analyses of six other tissue culture cell lines revealed cholesterol synthesis.

Bibliography

Rothblat, G. H., Burns, C. H., Conner, R. L., & Landrey, J. R. (1970). Desmosterol as the Major Sterol in L-Cell Mouse Fibroblasts Grown in Sterol-Free Culture Medium. Science, 169(3948), 880â882.

Authors 4

G. H. Rothblat (first)
C. H. Burns (additional)
R. L. Conner (additional)
J. R. Landrey (additional)

References 17 Referenced 56

BAILEY, J.M., LIPID METABOLISM IN CULTURED CELLS .6. LIPID BIOSYNTHESIS IN SERUM AND SYNTHETIC GROWTH MEDIA, BIOCHIMICA ET BIOPHYSICA ACTA 125: 226 (1966). (10.1016/0005-2760(66)90064-6) / BIOCHIMICA ET BIOPHYSICA ACTA (1966)
BAILEY, J.M., LIPID METABOLISM CUL: 85 (1967). / LIPID METABOLISM CUL (1967)
CONNER, R.L., CHOLESTEROL INHIBITION OF PENTACYCLIC TRITERPENOID BIOSYNTHESIS IN TETRAHYMENA PYRIFORMIS, JOURNAL OF PROTOZOOLOGY 15: 600 (1968). (10.1111/j.1550-7408.1968.tb02178.x) / JOURNAL OF PROTOZOOLOGY (1968)
CONNER, R.L., CONVERSION OF CHOLESTEROL TO DELTA5,7,22-CHOLESTATRIEN-3BETA-OL BY TETRAHYMENA PYRIFORMIS, JOURNAL OF BIOLOGICAL CHEMISTRY 244: 2325 (1969). (10.1016/S0021-9258(19)78229-5) / JOURNAL OF BIOLOGICAL CHEMISTRY (1969)
DEMPSEY, M.E., PATHWAYS OF ENZYMIC SYNTHESIS AND CONVERSION TO CHOLESTEROL OF DELTA5,7,24-CHOLESTATRIEN-3BETAO1 AND OTHER NATURALLY OCCURRING STEROLS, JOURNAL OF BIOLOGICAL CHEMISTRY 240: 4176 (1965). (10.1016/S0021-9258(18)97043-2) / JOURNAL OF BIOLOGICAL CHEMISTRY (1965)
DITULLIO, N.W., THIN-LAYER CHROMATOGRAPHY AND IDENTIFICATION OF FREE STEROLS, JOURNAL OF CHROMATOGRAPHY 20: 354 (1965). (10.1016/S0021-9673(01)97422-9) / JOURNAL OF CHROMATOGRAPHY (1965)
FUMAGALLI, E, J NEUROCHEM 11: 561 (1964). (10.1111/j.1471-4159.1964.tb11453.x) / J NEUROCHEM (1964)
HOLMES, R, CHOLESTEROL REQUIREMENT OF PRIMARY DIPLOID HUMAN FIBROBLASTS, JOURNAL OF CELL BIOLOGY 42: 262 (1969). (10.1083/jcb.42.1.262) / JOURNAL OF CELL BIOLOGY (1969)
ISSIDORIDES, C.H., CLEAVAGE OF STEROIDAL DIGITONIDES IN DIMETHYL SULFOXIDE, JOURNAL OF ORGANIC CHEMISTRY 27: 4693 (1962). (10.1021/jo01059a535) / JOURNAL OF ORGANIC CHEMISTRY (1962)
JOHNSTON, J.D., ISOLATION OF LANOSTEROL FROM ISOCHOLESTEROL, JOURNAL OF BIOLOGICAL CHEMISTRY 224: 185 (1957). (10.1016/S0021-9258(18)65020-3) / JOURNAL OF BIOLOGICAL CHEMISTRY (1957)
MACKENZIE, C.G., LIPID CONTENT + STRUCTURE OF CELLS, FEDERATION PROCEEDINGS 23: 375 (1964). / FEDERATION PROCEEDINGS (1964)
PAOLETTI, E.G., ADVAN EXP MED BIOL 4: 457 (1968). / ADVAN EXP MED BIOL (1968)
ROTHBLAT, G.H., ADV LIPID RES 7: 135 (1969). (10.1016/B978-0-12-024907-7.50010-5) / ADV LIPID RES (1969)
ROTHBLAT, G.H., METABOLISM OF FREE AND ESTERIFIED CHOLESTEROL IN TISSUE CULTURE CELLS - A REVIEW, EXPERIMENTAL AND MOLECULAR PATHOLOGY 8: 314 (1968). (10.1016/S0014-4800(68)80003-6) / EXPERIMENTAL AND MOLECULAR PATHOLOGY (1968)
ROTHBLAT, G, EFFECT OF SERUM COMPONENTS ON STEROL BIOSYNTHESIS IN L CELLS, JOURNAL OF CELLULAR PHYSIOLOGY 74: 163 (1969). / JOURNAL OF CELLULAR PHYSIOLOGY (1969)
SCALLEN, T.J., NUCLEAR MAGNETIC RESONANCE AND INFRARED SPECTRA OF DELTA24- AND C-24 SATURATED STEROIDS, JOURNAL OF LIPID RESEARCH 9: 120 (1968). (10.1016/S0022-2275(20)43152-9) / JOURNAL OF LIPID RESEARCH (1968)
VROMAN, H.E., SEPARATION OF STEROL ACETATES BY COLUMN AND THIN-LAYER ARGENTATION CHROMATOGRAPHY, JOURNAL OF LIPID RESEARCH 8: 150 (1967). (10.1016/S0022-2275(20)38930-6) / JOURNAL OF LIPID RESEARCH (1967)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:56 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 9:28 a.m.)
Indexed	1 year, 3 months ago (May 12, 2024, 7:23 p.m.)
Issued	55 years ago (Aug. 28, 1970)
Published	55 years ago (Aug. 28, 1970)
Published Print	55 years ago (Aug. 28, 1970)

Funders 0

None

BibTeX

@article{Rothblat_1970, title={Desmosterol as the Major Sterol in L-Cell Mouse Fibroblasts Grown in Sterol-Free Culture Medium}, volume={169}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.169.3948.880}, DOI={10.1126/science.169.3948.880}, number={3948}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Rothblat, G. H. and Burns, C. H. and Conner, R. L. and Landrey, J. R.}, year={1970}, month=aug, pages={880–882} }

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