DOI: 10.1126/science.168.3936.1220. Experimental Allergic Encephalomyelitis: Synthesis of Disease-Inducing Site of the Basic Protein

Experimental Allergic Encephalomyelitis: Synthesis of Disease-Inducing Site of the Basic Protein

10.1126/science.168.3936.1220

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A highly encephalitogenic peptide whose structure resembles the sequence of amino acids surrounding the single tryptophan residue in the encephalitogenic A1 protein from bovine myelin was synthesized. This peptide is similar in the sequence to peptic peptide E and tryptic T27, derived directly from the A1 protein, and is as active on a molar basis as the A1 protein. The major disease-inducing site of the A1 protein resides in a linear sequence of nine amino acids: H-Phe-Ser-Trp-Gly-Ala-Glu-Gly-Gln-Lys-OH. This region of the A1 protein is apparently the major encephalitogenic determinant since specific modification of the tryptophan residue in the A1 protein with 2-hydroxy-5-nitrobenzyl bromide destroyed its encephalitogenic activity.

Bibliography

Eylar, E. H., Caccam, J., Jackson, J. J., Westall, F. C., & Robinson, A. B. (1970). Experimental Allergic Encephalomyelitis: Synthesis of Disease-Inducing Site of the Basic Protein. Science, 168(3936), 1220â1223.

Authors 5

E. H. Eylar (first)
Juanita Caccam (additional)
J. J. Jackson (additional)
Fred C. Westall (additional)
Arthur B. Robinson (additional)

References 22 Referenced 145

Alvord E. C. The Central Nervous System (1968).
BARMAN, T.E., A COLORIMETRIC PROCEDURE FOR QUANTITATIVE DETERMINATION OF TRYPTOPHAN RESIDUES IN PROTEINS, JOURNAL OF BIOLOGICAL CHEMISTRY 242: 5771 (1967). (10.1016/S0021-9258(18)99367-1) / JOURNAL OF BIOLOGICAL CHEMISTRY (1967)
CARNEGIE, P.R., JOURNAL OF BIOCHEMISTRY 105: 559 (1967). (10.1042/bj1050559) / JOURNAL OF BIOCHEMISTRY (1967)
EYLAR, E.H., ALLERGIC ENCEPHALOMYELITIS . PHYSICO-CHEMICAL PROPERTIES OF BASIC PROTEIN ENCEPHALITOGEN FROM BOVINE SPINAL CORD, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 129: 468 (1969). (10.1016/0003-9861(69)90204-5) / ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (1969)
EYLAR, E.H., ALLERGIC ENCEPHALOMYELITIS - CLEAVAGE OF C-TRYPTOPHYL BOND IN ENCEPHALITOGENIC BASIC PROTEINFROM BOVINE MYELIN, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 131: 215 (1969). (10.1016/0003-9861(69)90124-6) / ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (1969)
EYLAR, E.H., EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS - AN ENCEPHALITOGENIC BASIC PROTEIN FROM BOVINE MYELIN, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 132: 34 (1969). (10.1016/0003-9861(69)90336-1) / ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (1969)
Eylar E. H. in preparation.
EYLAR, E.H., ALLERGIC ENCEPHALOMYELITIS - STRUCTURE OF ENCEPHALITOGENIC DETERMINANT, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 61: 644 (1968). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1968)
HASHIM, G.A., ALLERGIC ENCEPHALOMYELITIS . ENZYMATIC DEGRADATION OF ENCEPHALITOGENIC BASIC PROTEIN FROM BOVINE SPINAL CORD, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 129: 635 (1969). (10.1016/0003-9861(69)90224-0) / ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (1969)
HASHIM, G, ALLERGIC ENCEPHALOMYELITIS . ISOLATION AND CHARACTERIZATION OF ENCEPHALITOGENIC PEPTIDES FROM BASIC PROTEIN OF BOVINE SPINAL CORD, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 129: 645 (1969). (10.1016/0003-9861(69)90225-2) / ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (1969)
10.1126/science.164.3879.577
KIES, M.W., RELATIONSHIP OF MYELIN PROTEINS TO EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS, ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 122: 148 (1965). (10.1111/j.1749-6632.1965.tb20199.x) / ANNALS OF THE NEW YORK ACADEMY OF SCIENCES (1965)
KIES M PERSONAL COMMUNICATION.
KIES, M.W., CHEMICAL STUDIES ON AN EXCEPHALITOGENIC PROTEIN FROM GUINEA PIG BRAIN, ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 122: 161 (1965). (10.1111/j.1749-6632.1965.tb20200.x) / ANNALS OF THE NEW YORK ACADEMY OF SCIENCES (1965)
LENARD, J, USE OF HYDROGEN FLUORIDE IN MERRIFIELD SOLID-PHASE PEPTIDE SYNTHESIS, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 89: 181 (1967). (10.1021/ja00977a057) / JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (1967)
MARGLIN, A, SYNTHESIS OF BOVINE INSULIN BY SOLID PHASE METHOD, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 88: 5051 (1966). (10.1021/ja00973a068) / JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (1966)
MARSHALL, G.R., SYNTHESIS OF ANGIOTENSINS BY SOLID-PHASE METHOD, BIOCHEMISTRY 4: 2394 (1965). (10.1021/bi00887a020) / BIOCHEMISTRY (1965)
MERRIFIELD, R.B., SOLID PHASE PEPTIDE SYNTHESIS .1. SYNTHESIS OF A TETRAPEPTIDE, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 85: 2149 (1963). (10.1021/ja00897a025) / JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (1963)
NAKAO, A, BIOCHIMICA ET BIOPHYSICA ACTA 130: 163 (1966). (10.1016/0304-4165(66)90019-5) / BIOCHIMICA ET BIOPHYSICA ACTA (1966)
Oshiro, Y., Archives of Biochemistry and Biophysics 138: 392 (1970). (10.1016/0003-9861(70)90361-9) / Archives of Biochemistry and Biophysics (1970)
PALMER, F.B., ISOLATION AND PROPERTIES OF EXPERIMENTAL ALLERGIC ENCEPHALITOGENIC PROTEIN, BIOCHEMICAL JOURNAL 111: 629 (1969). (10.1042/bj1110629) / BIOCHEMICAL JOURNAL (1969)
SAKAKIBARA, S, USE OF ANHYDROUS HYDROGEN FLUORIDE IN PEPTIDE SYNTHESIS .I. BEHAVIOR OF VARIOUS PROTECTIVE GROUPS IN ANHYDROUS HYDROGEN FLUORIDE, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 40: 2164 (1967). (10.1246/bcsj.40.2164) / BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN (1967)

Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 8:56 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 10:36 a.m.)
Indexed	2 days, 6 hours ago (Sept. 4, 2025, 9:28 a.m.)
Issued	55 years, 3 months ago (June 5, 1970)
Published	55 years, 3 months ago (June 5, 1970)
Published Print	55 years, 3 months ago (June 5, 1970)

Funders 0

None

BibTeX

@article{Eylar_1970, title={Experimental Allergic Encephalomyelitis: Synthesis of Disease-Inducing Site of the Basic Protein}, volume={168}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.168.3936.1220}, DOI={10.1126/science.168.3936.1220}, number={3936}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Eylar, E. H. and Caccam, Juanita and Jackson, J. J. and Westall, Fred C. and Robinson, Arthur B.}, year={1970}, month=jun, pages={1220–1223} }

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