DOI: 10.1126/science.1652153. Variable Effects of Phosphorylation of Pit-1 Dictated by the DNA Response Elements

Variable Effects of Phosphorylation of Pit-1 Dictated by the DNA Response Elements

10.1126/science.1652153

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Pit-1, a tissue-specific POU domain transcription factor, is required for the activation of the prolactin, growth hormone, and Pit-1 promoters that confer regulation by epidermal growth factor, adenosine 3′,5′-monophosphate (cAMP), and phorbol esters. Pit-1 is phosphorylated in pituitary cells at two distinct sites in response to phorbol esters and cAMP. Phosphorylation of Pit-1 modifies its conformation on DNA recognition elements and results in increased binding at certain sites and decreased binding at other sites, dependent on DNA sequences adjacent to the core Pit-1 binding motif. One residue (Thr 220 ), located in the POU homeodomain within a sequence conserved throughout the POU-domain family, confers these responses.

Bibliography

Kapiloff, M. S., Farkash, Y., Wegner, M., & Rosenfeld, M. G. (1991). Variable Effects of Phosphorylation of Pit-1 Dictated by the DNA Response Elements. Science, 253(5021), 786â789.

Authors 4

Michael S. Kapiloff (first)
Yigal Farkash (additional)
Michael Wegner (additional)
Michael G. Rosenfeld (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 11:17 a.m.)
Indexed	3 months ago (May 22, 2025, 2:46 a.m.)
Issued	34 years ago (Aug. 16, 1991)
Published	34 years ago (Aug. 16, 1991)
Published Print	34 years ago (Aug. 16, 1991)

Funders 0

None

BibTeX

@article{Kapiloff_1991, title={Variable Effects of Phosphorylation of Pit-1 Dictated by the DNA Response Elements}, volume={253}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1652153}, DOI={10.1126/science.1652153}, number={5021}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Kapiloff, Michael S. and Farkash, Yigal and Wegner, Michael and Rosenfeld, Michael G.}, year={1991}, month=aug, pages={786–789} }

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