DOI: 10.1126/science.165.3899.1260. Heterogeneity of Presumably Homogeneous Protein Preparations

Heterogeneity of Presumably Homogeneous Protein Preparations

10.1126/science.165.3899.1260

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Some highly purified glycolytic enzymes have been subjected to isoelectric focusing and found to contain a number of enzymatically active species. Crystalline aldolase A and glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle were resolved into five components, crystalline aldolase from yeast was resolved into three components, pyruvate kinase from rabbit muscle yielded four components, and yeast enolase was resolved into two components. Rabbit muscle lactate dehydrogenase (M 4 ) gave one major peak of protein and enzymatic activity. The profiles of aldolase, glyceraldehyde-3-phosphate dehydrogenase, and yeast aldolases suggest random combinations of two closely related subunits into tetramers and dimers, respectively. The molecular heterogeneity of the other enzymes is not so easily related to subunit structure.

Bibliography

Susor, W. A., Kochman, M., & Rutter, W. J. (1969). Heterogeneity of Presumably Homogeneous Protein Preparations. Science, 165(3899), 1260â1262.

Authors 3

Walter A. Susor (first)
Marion Kochman (additional)
William J. Rutter (additional)

References 29 Referenced 95

BLOSTEIN, R, COMPARATIVE STUDIES OF LIVER AND MUSCLE ALDOLASE .2. IMMUNOCHEMICAL AND CHROMATOGRAPHIC DIFFERENTIATION, JOURNAL OF BIOLOGICAL CHEMISTRY 238: 3280 (1963). (10.1016/S0021-9258(18)48659-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1963)
BUCHER, T, PYRUVATE KINASE FROM MUSCLE, METHODS IN ENZYMOLOGY 1: 435 (1955). (10.1016/0076-6879(55)01071-9) / METHODS IN ENZYMOLOGY (1955)
CHAN, W, NONIDENTITY OF SUBUNITS OF RABBIT MUSCLE ALDOLASE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 57: 1013 (1967). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1967)
CORI, G.T., CRYSTALLINE D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM RABBIT MUSCLE, JOURNAL OF BIOLOGICAL CHEMISTRY 173: 605 (1948). (10.1016/S0021-9258(18)57432-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1948)
DEAL, W.C., SUBNIT STRUCTURE OF SOME GLYCOLYTIC ENZYMES, FEDERATION PROCEEDINGS 23: 264 (1964). / FEDERATION PROCEEDINGS (1964)
EDELSTEIN, S.J., STUDIES ON POLYPEPTIDE CHAINS OF RABBIT MUSCLE ALDOLASE, FEDERATION PROCEEDINGS 25: 412 (1966). / FEDERATION PROCEEDINGS (1966)
GELDERMAN, A. H., JOURNAL OF LABORATORY AND CLINICAL MEDICINE 65: 132 (1965). / JOURNAL OF LABORATORY AND CLINICAL MEDICINE (1965)
HARGRAVE P.A. PERSONAL COMMUNICATION.
Harris, C. E., Biochemistry 8: 2442 (1969). (10.1021/bi00834a029) / Biochemistry (1969)
HARRIS, J.I., GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASES .1. PROTEIN CHAINS IN GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE FROM PIG MUSCLE, JOURNAL OF MOLECULAR BIOLOGY 13: 876 (1965). (10.1016/S0022-2836(65)80151-6) / JOURNAL OF MOLECULAR BIOLOGY (1965)
KOCHMAN, M, SIMILARITY OF GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASES ISOLATED FROM RABBIT BRAIN AND MUSCLE, BIOCHEMISTRY 7: 1671 (1968). (10.1021/bi00845a008) / BIOCHEMISTRY (1968)
KOCHMAN, M, FEDERATION PROCEEDINGS 27: 590 (1968). / FEDERATION PROCEEDINGS (1968)
Kochman M. in preparation..
KORNBERG, A, LACTIC DEHYDROGENASE OF MUSCLE, METHODS IN ENZYMOLOGY 1: 441 (1955). (10.1016/0076-6879(55)01072-0) / METHODS IN ENZYMOLOGY (1955)
10.1016/S0021-9258(19)67912-3
Penhoet, E., Biochemistry 8: 4391 (1969). (10.1021/bi00839a025) / Biochemistry (1969)
PENHOET, E, MULTIPLE FORMS OF FRUCTOSE DIPHOSPHATE ALDOLASE IN MAMMALIAN TISSUES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 56: 1275 (1966). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1966)
PLUMMER, T.H., ON STRUCTURE OF BOVINE PANCREATIC RIBONUCLEASE B - ISOLATION OF GLYCOPEPTIDE, JOURNAL OF BIOLOGICAL CHEMISTRY 239: 2530 (1964). (10.1016/S0021-9258(18)93883-4) / JOURNAL OF BIOLOGICAL CHEMISTRY (1964)
POGELL, B.N., ENZYME PURIFICATION BY SELECTIVE ELUTION WITH SUBSTRATE FROM SUBSTITUTED CELLULOSE COLUMNS, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 7: 225 (1962). (10.1016/0006-291X(62)90179-1) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1962)
RAJKUMAR, E, FEDERATION PROCEEDINGS 25: 523 (1966). / FEDERATION PROCEEDINGS (1966)
RICHARDS, O.C., PREPARATION AND PROPERTIES OF YEAST ALDOLASE, JOURNAL OF BIOLOGICAL CHEMISTRY 236: 3177 (1961). (10.1016/S0021-9258(18)93992-X) / JOURNAL OF BIOLOGICAL CHEMISTRY (1961)
RUTTER, W.J., Fructose diphosphate aldolase, METHODS IN ENZYMOLOGY 9: 479 (1966). (10.1016/0076-6879(66)09099-2) / METHODS IN ENZYMOLOGY / Fructose diphosphate aldolase (1966)
TANAKA, T, CRYSTALLIZATION CHARACTERIZATION AND METABOLIC REGULATION OF 2 TYPES OF PYRUVATE KINASE ISOLATED FROM RAT TISSUES, JOURNAL OF BIOCHEMISTRY 62: 71 (1967). (10.1093/oxfordjournals.jbchem.a128639) / JOURNAL OF BIOCHEMISTRY (1967)
TIETZ, A, FLUOROKINASE AND PYRUVIC KINASE, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 78: 477 (1958). (10.1016/0003-9861(58)90372-2) / ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (1958)
VELICK, S.F., GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM MUSCLE, METHODS IN ENZYMOLOGY 1: 401 (1955). (10.1016/0076-6879(55)01065-3) / METHODS IN ENZYMOLOGY (1955)
VESTERBERG, O, ISOELECTRIC FRACTIONATION ANALYSIS AND CHARACTERIZATION OF AMPHOLYTES IN NATURAL PH GRADIENTS .4. FURTHER STUDIES ON RESOLVING POWER IN CONNECTION WITH SEPARATION OF MYOGLOBINS, ACTA CHEMICA SCANDINAVICA 20: 820 (1966). (10.3891/acta.chem.scand.20-0820) / ACTA CHEMICA SCANDINAVICA (1966)
WESTHEAD, E.W., PURIFICATION OF BREWERS + BAKERS YEAST ENOLASE YIELDING SINGLE ACTIVE COMPONENT, JOURNAL OF BIOLOGICAL CHEMISTRY 239: 2464 (1964). (10.1016/S0021-9258(18)93875-5) / JOURNAL OF BIOLOGICAL CHEMISTRY (1964)
WESTHEAD, E.W., METHODS IN ENZYMOLOGY 9: 670 (1966). (10.1016/0076-6879(66)09136-5) / METHODS IN ENZYMOLOGY (1966)
10.1016/S0021-9258(18)91158-0

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:14 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 9:11 a.m.)
Indexed	11 months, 2 weeks ago (Sept. 15, 2024, 5:21 p.m.)
Issued	55 years, 11 months ago (Sept. 19, 1969)
Published	55 years, 11 months ago (Sept. 19, 1969)
Published Print	55 years, 11 months ago (Sept. 19, 1969)

Funders 0

None

BibTeX

@article{Susor_1969, title={Heterogeneity of Presumably Homogeneous Protein Preparations}, volume={165}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.165.3899.1260}, DOI={10.1126/science.165.3899.1260}, number={3899}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Susor, Walter A. and Kochman, Marion and Rutter, William J.}, year={1969}, month=sep, pages={1260–1262} }

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    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1969,
        9,
        19
      ]
    ]
  }
}