DOI: 10.1126/science.1648264. Global Suppression of Protein Folding Defects and Inclusion Body Formation

Global Suppression of Protein Folding Defects and Inclusion Body Formation

10.1126/science.1648264

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Amino acid substitutions at a site in the center of the bacteriophage protein P22 tailspike polypeptide chain suppress temperature-sensitive folding mutations at many sites throughout the chain. Characterization of the intracellular folding and chain assembly process reveals that the suppressors act in the folding pathway, inhibiting the aggregation of an early folding intermediate into the kinetically trapped inclusion body state. The suppressors alone increase the folding efficiency of the otherwise wild-type polypeptide chain without altering the stability or activity of the native state. These amino acid substitutions identify an unexpected aspect of the protein folding grammar—sequences within the chain that carry information inhibiting unproductive off-pathway conformations. Such mutations may serve to increase the recovery of protein products of cloned genes.

Bibliography

Mitraki, A., Fane, B., Haase-Pettingell, C., Sturtevant, J., & King, J. (1991). Global Suppression of Protein Folding Defects and Inclusion Body Formation. Science, 253(5015), 54â58.

Authors 5

Anna Mitraki (first)
Bentley Fane (additional)
Cameron Haase-Pettingell (additional)
Julian Sturtevant (additional)
Jonathan King (additional)

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Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 10:32 a.m.)
Indexed	2 months, 1 week ago (June 27, 2025, 6:14 a.m.)
Issued	34 years, 2 months ago (July 5, 1991)
Published	34 years, 2 months ago (July 5, 1991)
Published Print	34 years, 2 months ago (July 5, 1991)

Funders 0

None

BibTeX

@article{Mitraki_1991, title={Global Suppression of Protein Folding Defects and Inclusion Body Formation}, volume={253}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1648264}, DOI={10.1126/science.1648264}, number={5015}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Mitraki, Anna and Fane, Bentley and Haase-Pettingell, Cameron and Sturtevant, Julian and King, Jonathan}, year={1991}, month=jul, pages={54–58} }

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