DOI: 10.1126/science.1636086. Magnetoferritin: in Vitro Synthesis of a Novel Magnetic Protein

Magnetoferritin: in Vitro Synthesis of a Novel Magnetic Protein

10.1126/science.1636086

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The iron storage protein ferritin consists of a spherical polypeptide shell (apoferritin) surrounding a 6-nanometer inorganic core of the hydrated iron oxide ferrihydrite (5Fe 2 O 3 ⋅9H 2 O). Previous studies have shown that the in vitro reconstitution of apoferritin yields mineral cores essentially identical to those of the native proteins. A magnetic mineral was synthesized within the nanodimensional cavity of horse spleen ferritin by the use of controlled reconstitution conditions. Transmission electron microscopy and electron diffraction analysis indicate that the entrapped mineral particles are discrete 6-nanometer spherical single crystals of the ferrimagnetic iron oxide magnetite (Fe 3 O 4 ). The resulting magnetic protein, "magnetoferritin," could have uses in biomedical imaging, cell labeling, and separation procedures.

Bibliography

Meldrum, F. C., Heywood, B. R., & Mann, S. (1992). Magnetoferritin: in Vitro Synthesis of a Novel Magnetic Protein. Science, 257(5069), 522â523.

Authors 3

Fiona C. Meldrum (first)
Brigid R. Heywood (additional)
Stephen Mann (additional)

References 20 Referenced 515

BUTLER, R. F., JOURNAL OF GEOPHYSICAL RESEARCH 80: 4049 (1975). (10.1029/JB080i029p04049) / JOURNAL OF GEOPHYSICAL RESEARCH (1975)
FORD, G.C., FERRITIN - DESIGN AND FORMATION OF AN IRON-STORAGE MOLECULE, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES 304: 551 (1984). / PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES (1984)
Kemp, W., NMR in Chemistry: A Multinuclear Introduction: 154 (1986). / NMR in Chemistry: A Multinuclear Introduction (1986)
KIYAMA, M, CONDITIONS FOR FORMATION OF FE3O4 BY AIR OXIDATION OF FE(OH)2 SUSPENSIONS, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 47: 1646 (1974). (10.1246/bcsj.47.1646) / BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN (1974)
LAWSON, D.M., IDENTIFICATION OF THE FERROXIDASE CENTER IN FERRITIN, FEBS LETTERS 254: 207 (1989). (10.1016/0014-5793(89)81040-3) / FEBS LETTERS (1989)
LAWSON, D.M., SOLVING THE STRUCTURE OF HUMAN H-FERRITIN BY GENETICALLY ENGINEERING INTERMOLECULAR CRYSTAL CONTACTS, NATURE 349: 541 (1991). (10.1038/349541a0) / NATURE (1991)
LEVI, S, MECHANISM OF FERRITIN IRON UPTAKE - ACTIVITY OF THE H-CHAIN AND DELETION MAPPING OF THE FERRO-OXIDASE SITE - A STUDY OF IRON UPTAKE AND FERRO-OXIDASE ACTIVITY OF HUMAN-LIVER, RECOMBINANT H-CHAIN FERRITINS, AND OF 2 H-CHAIN DELETION MUTANTS, JOURNAL OF BIOLOGICAL CHEMISTRY 263: 18086 (1988). (10.1016/S0021-9258(19)81326-1) / JOURNAL OF BIOLOGICAL CHEMISTRY (1988)
LIVNE, E, INTERNALIZATION OF CATIONIZED FERRITIN BY ISOLATED PANCREATIC ACINAR-CELLS, JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 34: 167 (1986). (10.1177/34.2.3944455) / JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY (1986)
Mann S. Biomineralization: Chemical and Biochemical Perspectives (1989).
MANN, S, CRYSTALLOCHEMICAL CHARACTERIZATION OF MAGNETIC SPINELS PREPARED FROM AQUEOUS-SOLUTION, JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS I 85: 3033 (1989). (10.1039/f19898503033) / JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS I (1989)
MANN, S, RECONSTITUTED AND NATIVE IRON-CORES OF BACTERIOFERRITIN AND FERRITIN, JOURNAL OF MOLECULAR BIOLOGY 198: 405 (1987). (10.1016/0022-2836(87)90290-7) / JOURNAL OF MOLECULAR BIOLOGY (1987)
10.1038/349684a0
MELDRUM F.C. unpublished data.
MOLDAY, R.S., APPLICATION OF MAGNETIC MICROSPHERES IN LABELING AND SEPARATION OF CELLS, NATURE 268: 437 (1977). (10.1038/268437a0) / NATURE (1977)
OZAKI, M, PREPARATION AND MAGNETIC-PROPERTIES OF MONODISPERSED SPINDLE-TYPE GAMMA-FE2O3 PARTICLES, JOURNAL OF COLLOID AND INTERFACE SCIENCE 107: 199 (1985). (10.1016/0021-9797(85)90162-6) / JOURNAL OF COLLOID AND INTERFACE SCIENCE (1985)
TAMAURA, Y, JOURNAL OF THE CHEMICAL SOCIETY-DALTON TRANSACTIONS: 1807 (1981). / JOURNAL OF THE CHEMICAL SOCIETY-DALTON TRANSACTIONS (1981)
TAMAURA, Y, JOURNAL OF THE CHEMICAL SOCIETY-DALTON TRANSACTIONS: 189 (1983). / JOURNAL OF THE CHEMICAL SOCIETY-DALTON TRANSACTIONS (1983)
TAYLOR, R.M., INFLUENCE OF CHLORIDE ON THE FORMATION OF IRON-OXIDES FROM FE(II) CHLORIDE .1. EFFECT OF [CL]/[FE] ON THE FORMATION OF MAGNETITE, CLAYS AND CLAY MINERALS 32: 167 (1984). (10.1346/CCMN.1984.0320302) / CLAYS AND CLAY MINERALS (1984)
WADE, V.J., INFLUENCE OF SITE-DIRECTED MODIFICATIONS ON THE FORMATION OF IRON CORES IN FERRITIN, JOURNAL OF MOLECULAR BIOLOGY 221: 1443 (1991). (10.1016/0022-2836(91)90944-2) / JOURNAL OF MOLECULAR BIOLOGY (1991)
ZHAO, X. K., JOURNAL OF PHYSICAL CHEMISTRY 94: 2573 (1990). (10.1021/j100369a065) / JOURNAL OF PHYSICAL CHEMISTRY (1990)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 10:32 a.m.)
Indexed	2 weeks, 3 days ago (Aug. 7, 2025, 4:55 a.m.)
Issued	33 years, 1 month ago (July 24, 1992)
Published	33 years, 1 month ago (July 24, 1992)
Published Print	33 years, 1 month ago (July 24, 1992)

Funders 0

None

BibTeX

@article{Meldrum_1992, title={Magnetoferritin: in Vitro Synthesis of a Novel Magnetic Protein}, volume={257}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1636086}, DOI={10.1126/science.1636086}, number={5069}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Meldrum, Fiona C. and Heywood, Brigid R. and Mann, Stephen}, year={1992}, month=jul, pages={522–523} }

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