DOI: 10.1126/science.1631557. Crystal Structure of Transforming Growth Factor-β2: an Unusual Fold for the Superfamily

Crystal Structure of Transforming Growth Factor-β2: an Unusual Fold for the Superfamily

10.1126/science.1631557

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The transforming growth factors-β (TGF-β1 through -β5) are a family of homodimeric cytokines that regulate proliferation and function in many cell types. Family members have 66 to 80% sequence identity and nine strictly conserved cysteines. A crystal structure of a member of this family, TGF-β2, has been determined at 2.1 angstrom (Å) resolution and refined to an R factor of 0.172. The monomer lacks a well-defined hydrophobic core and displays an unusual elongated nonglobular fold with dimensions of approximately 60 Å by 20 Å by 15 Å. Eight cysteines form four intrachain disulfide bonds, which are clustered in a core region forming a network complementary to the network of hydrogen bonds. The dimer is stabilized by the ninth cysteine, which forms an interchain disulfide bond, and by two identical hydrophobic interfaces. Sequence profile analysis of other members of the TGF-β superfamily, including the activins, inhibins, and several developmental factors, imply that they also adopt the TGF-β fold.

Bibliography

Daopin, S., Piez, K. A., Ogawa, Y., & Davies, D. R. (1992). Crystal Structure of Transforming Growth Factor-Î²2: an Unusual Fold for the Superfamily. Science, 257(5068), 369â373.

Authors 4

Sun Daopin (first)
Karl A. Piez (additional)
Yasushi Ogawa (additional)
David R. Davies (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 10:34 a.m.)
Indexed	1 month, 2 weeks ago (July 8, 2025, 7:30 a.m.)
Issued	33 years, 1 month ago (July 17, 1992)
Published	33 years, 1 month ago (July 17, 1992)
Published Print	33 years, 1 month ago (July 17, 1992)

Funders 0

None

BibTeX

@article{Daopin_1992, title={Crystal Structure of Transforming Growth Factor-β2: an Unusual Fold for the Superfamily}, volume={257}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1631557}, DOI={10.1126/science.1631557}, number={5068}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Daopin, Sun and Piez, Karl A. and Ogawa, Yasushi and Davies, David R.}, year={1992}, month=jul, pages={369–373} }

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