DOI: 10.1126/science.162.3856.909. Structure of Human Serum Lipoproteins: Nuclear Magnetic Resonance Supports a Micellar Model

Structure of Human Serum Lipoproteins: Nuclear Magnetic Resonance Supports a Micellar Model

10.1126/science.162.3856.909

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

High-resolution proton nuclear magnetic resonance spectra of low- and high-density lipoproteins from human serum closely resemble those of dispersions of lipoprotein lipids in water. Linewidths of hydrocarbon proton absorptions are not increased in the lipoproteins. In contrast, apolar binding of lysolecithin on serum albumin causes extensive line-broadening and an upfield chemical shift of the hydrocarbon proton resonances of lysolecithin. The results are consistent with a predominantly micellar structure for the lipoproteins rather than with extensive hydrophobic association of lipid and protein.

Bibliography

Steim, J. M., Edner, O. J., & Bargoot, F. G. (1968). Structure of Human Serum Lipoproteins: Nuclear Magnetic Resonance Supports a Micellar Model. Science, 162(3856), 909â911.

Authors 3

Joseph M. Steim (first)
Orville J. Edner (additional)
Frederick G. Bargoot (additional)

References 10 Referenced 75

CHAPMAN, D, CHEM PHYS LIPIDS 1: 445 (1967). (10.1016/0009-3084(67)90023-0) / CHEM PHYS LIPIDS (1967)
CHAPMAN, D, PHYSICAL STUDIES OF PHOSPHOLIPIDS .4. HIGH RESOLUTION NUCLEAR MAGNETIC RESONANCE SPECTRA OF PHOSPHOLIPIDS AND RELATED SUBSTANCES, JOURNAL OF BIOLOGICAL CHEMISTRY 241: 5044 (1966). (10.1016/S0021-9258(18)99668-7) / JOURNAL OF BIOLOGICAL CHEMISTRY (1966)
CHAPMAN, D, NUCLEAR MAGNETIC RESONANCE STUDIES OF ERYTHROCYTE MEMBRANES, JOURNAL OF MOLECULAR BIOLOGY 31: 101 (1968). (10.1016/0022-2836(68)90058-2) / JOURNAL OF MOLECULAR BIOLOGY (1968)
CHEN, P.S., MICRODETERMINATION OF PHOSPHORUS, ANALYTICAL CHEMISTRY 28: 1756 (1956). (10.1021/ac60119a033) / ANALYTICAL CHEMISTRY (1956)
CLIFFORD, J, PROPERTIES OF MICELLAR SOLUTIONS .2. NMR CHEMICAL SHIFT OF WATER PROTONS IN SOLUTIONS OF SODIUM ALKYL SULPHATES, TRANSACTIONS OF THE FARADAY SOCIETY 60: 1483 (1964). (10.1039/TF9646001483) / TRANSACTIONS OF THE FARADAY SOCIETY (1964)
FOLCH, J, A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES, JOURNAL OF BIOLOGICAL CHEMISTRY 226: 497 (1957). (10.1016/S0021-9258(18)64849-5) / JOURNAL OF BIOLOGICAL CHEMISTRY (1957)
GERIG, J.T., NUCLEAR MAGNETIC RESONANCE STUDIES OF INTERACTION OF TRYPTOPHAN WITH ALPHA-CHYMOTRYPSIN, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 90: 2681 (1968). (10.1021/ja01012a038) / JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (1968)
REISSHUSSON, F, ADV BIOL MED PHYS 11: 87 (1967). (10.1016/B978-1-4832-3107-5.50007-8) / ADV BIOL MED PHYS (1967)
SAUNDERS, L, MOLECULAR AGGREGATION IN AQUEOUS DISPERSIONS OF PHOSPHATIDYL AND LYSOPHOSPHATIDYL CHOLINES, BIOCHIMICA ET BIOPHYSICA ACTA 125: 70 (1966). (10.1016/0005-2760(66)90145-7) / BIOCHIMICA ET BIOPHYSICA ACTA (1966)
SCANU, A. M., Factors Affecting Lipoprotein Metabolism, ADVANCES IN LIPID RESEARCH 3: 63 (1965). / ADVANCES IN LIPID RESEARCH / Factors Affecting Lipoprotein Metabolism (1965)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:50 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 3:38 a.m.)
Indexed	1 year, 7 months ago (Jan. 11, 2024, 7:05 p.m.)
Issued	56 years, 9 months ago (Nov. 22, 1968)
Published	56 years, 9 months ago (Nov. 22, 1968)
Published Print	56 years, 9 months ago (Nov. 22, 1968)

Funders 0

None

BibTeX

@article{Steim_1968, title={Structure of Human Serum Lipoproteins: Nuclear Magnetic Resonance Supports a Micellar Model}, volume={162}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.162.3856.909}, DOI={10.1126/science.162.3856.909}, number={3856}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Steim, Joseph M. and Edner, Orville J. and Bargoot, Frederick G.}, year={1968}, month=nov, pages={909–911} }

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