DOI: 10.1126/science.161.3839.370. Differentiated Rat Glial Cell Strain in Tissue Culture

Differentiated Rat Glial Cell Strain in Tissue Culture

10.1126/science.161.3839.370

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Rat glial tumors, induced by injections of N -nitrosomethylurea, were plated and propagated in culture. Among a few cell strains obtained, one clone contains S-100 protein, which is unique to brain in vertebrates. Stationary-phase cultures contain approximately ten times more S-100 protein per cell than exponentially growing cells. When injected into newborn rats, cells producing S-100 grew as a glial tumor, which contained S-100 protein.

Bibliography

Benda, P., Lightbody, J., Sato, G., Levine, L., & Sweet, W. (1968). Differentiated Rat Glial Cell Strain in Tissue Culture. Science, 161(3839), 370â371.

Authors 5

Philippe Benda (first)
James Lightbody (additional)
Gordon Sato (additional)
Lawrence Levine (additional)
William Sweet (additional)

References 11 Referenced 1,185

BENDA P.F. UNPUBLISHED OBSERVATIONS.
BUONASSISI, V, HORMONE-PRODUCING CULTURES OF ADRENAL AND PITUITARY TUMOR ORIGIN, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 48: 1184 (1962). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1962)
10.1126/science.156.3777.907
DRUCKREY, H, ZEITSCHRIFT FUR KREBSFORSCHUNG 66: 389 (1965). (10.1007/BF00525737) / ZEITSCHRIFT FUR KREBSFORSCHUNG (1965)
HAM, R.G., AN IMPROVED NUTRIENT SOLUTION FOR DIPLOID CHINESE HAMSTER AND HUMAN CELL LINES, EXPERIMENTAL CELL RESEARCH 29: 515 (1963). (10.1016/S0014-4827(63)80014-2) / EXPERIMENTAL CELL RESEARCH (1963)
KESSLER, D, SOME CONFORMATIONAL AND IMMUNOLOGICAL PROPERTIES OF A BOVINE BRAIN ACIDIC PROTEIN (S-100), BIOCHEMISTRY 7: 758 (1968). (10.1021/bi00842a034) / BIOCHEMISTRY (1968)
Levine, L., Handbook of Experimental Immunology: 707 (1968). / Handbook of Experimental Immunology (1968)
LEVINE, L, NEUROSCIENCES RESEARCH PROGRAM BULLETIN 3: 18 (1965). / NEUROSCIENCES RESEARCH PROGRAM BULLETIN (1965)
10.1016/S0021-9258(19)52451-6
MOORE, B.W., A SOLUBLE PROTEIN CHARACTERISTIC OF NERVOUS SYSTEM, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 19: 739 (1965). (10.1016/0006-291X(65)90320-7) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1965)
MOORE, B.W., CHROMATOGRAPHIC AND ELECTROPHORECTIC FRACTIONATION OF SOLUBLE PROTEINS OF BRAIN AND LIVER, JOURNAL OF BIOLOGICAL CHEMISTRY 240: 1647 (1965). (10.1016/S0021-9258(18)97483-1) / JOURNAL OF BIOLOGICAL CHEMISTRY (1965)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:50 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 4:06 a.m.)
Indexed	2 days, 11 hours ago (Aug. 30, 2025, 12:27 p.m.)
Issued	57 years, 1 month ago (July 26, 1968)
Published	57 years, 1 month ago (July 26, 1968)
Published Print	57 years, 1 month ago (July 26, 1968)

Funders 0

None

BibTeX

@article{Benda_1968, title={Differentiated Rat Glial Cell Strain in Tissue Culture}, volume={161}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.161.3839.370}, DOI={10.1126/science.161.3839.370}, number={3839}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Benda, Philippe and Lightbody, James and Sato, Gordon and Levine, Lawrence and Sweet, William}, year={1968}, month=jul, pages={370–371} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        30
      ]
    ],
    "date-time": "2025-08-30T16:27:32Z",
    "timestamp": 1756571252225
  },
  "reference-count": 11,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "3839",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1968,
        7,
        26
      ]
    ]
  },
  "abstract": "<jats:p>\n            Rat glial tumors, induced by injections of\n            <jats:italic>N</jats:italic>\n            -nitrosomethylurea, were plated and propagated in culture. Among a few cell strains obtained, one clone contains S-100 protein, which is unique to brain in vertebrates. Stationary-phase cultures contain approximately ten times more S-100 protein per cell than exponentially growing cells. When injected into newborn rats, cells producing S-100 grew as a glial tumor, which contained S-100 protein.\n          </jats:p>",
  "DOI": "10.1126/science.161.3839.370",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        10,
        5
      ]
    ],
    "date-time": "2006-10-05T11:50:27Z",
    "timestamp": 1160049027000
  },
  "page": "370-371",
  "source": "Crossref",
  "is-referenced-by-count": 1185,
  "title": "Differentiated Rat Glial Cell Strain in Tissue Culture",
  "prefix": "10.1126",
  "volume": "161",
  "author": [
    {
      "given": "Philippe",
      "family": "Benda",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02154 and Neurosurgical Service, Massachusetts General Hospital, Boston, Massachusetts"
        }
      ]
    },
    {
      "given": "James",
      "family": "Lightbody",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02154 and Neurosurgical Service, Massachusetts General Hospital, Boston, Massachusetts"
        }
      ]
    },
    {
      "given": "Gordon",
      "family": "Sato",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02154 and Neurosurgical Service, Massachusetts General Hospital, Boston, Massachusetts"
        }
      ]
    },
    {
      "given": "Lawrence",
      "family": "Levine",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02154 and Neurosurgical Service, Massachusetts General Hospital, Boston, Massachusetts"
        }
      ]
    },
    {
      "given": "William",
      "family": "Sweet",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02154 and Neurosurgical Service, Massachusetts General Hospital, Boston, Massachusetts"
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_2_1_1_1",
      "unstructured": "BENDA P.F. UNPUBLISHED OBSERVATIONS."
    },
    {
      "key": "e_1_2_1_2_1",
      "first-page": "1184",
      "volume": "48",
      "year": "1962",
      "unstructured": "BUONASSISI, V, HORMONE-PRODUCING CULTURES OF ADRENAL AND PITUITARY TUMOR ORIGIN, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 48: 1184 (1962).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.156.3777.907"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "crossref",
      "first-page": "389",
      "DOI": "10.1007/BF00525737",
      "volume": "66",
      "year": "1965",
      "unstructured": "DRUCKREY, H, ZEITSCHRIFT FUR KREBSFORSCHUNG 66: 389 (1965).",
      "journal-title": "ZEITSCHRIFT FUR KREBSFORSCHUNG"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "crossref",
      "first-page": "515",
      "DOI": "10.1016/S0014-4827(63)80014-2",
      "volume": "29",
      "year": "1963",
      "unstructured": "HAM, R.G., AN IMPROVED NUTRIENT SOLUTION FOR DIPLOID CHINESE HAMSTER AND HUMAN CELL LINES, EXPERIMENTAL CELL RESEARCH 29: 515 (1963).",
      "journal-title": "EXPERIMENTAL CELL RESEARCH"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "crossref",
      "first-page": "758",
      "DOI": "10.1021/bi00842a034",
      "volume": "7",
      "year": "1968",
      "unstructured": "KESSLER, D, SOME CONFORMATIONAL AND IMMUNOLOGICAL PROPERTIES OF A BOVINE BRAIN ACIDIC PROTEIN (S-100), BIOCHEMISTRY 7: 758 (1968).",
      "journal-title": "BIOCHEMISTRY"
    },
    {
      "key": "e_1_2_1_7_1",
      "first-page": "707",
      "year": "1968",
      "unstructured": "Levine, L., Handbook of Experimental Immunology: 707 (1968).",
      "journal-title": "Handbook of Experimental Immunology"
    },
    {
      "key": "e_1_2_1_8_1",
      "first-page": "18",
      "volume": "3",
      "year": "1965",
      "unstructured": "LEVINE, L, NEUROSCIENCES RESEARCH PROGRAM BULLETIN 3: 18 (1965).",
      "journal-title": "NEUROSCIENCES RESEARCH PROGRAM BULLETIN"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)52451-6"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "crossref",
      "first-page": "739",
      "DOI": "10.1016/0006-291X(65)90320-7",
      "volume": "19",
      "year": "1965",
      "unstructured": "MOORE, B.W., A SOLUBLE PROTEIN CHARACTERISTIC OF NERVOUS SYSTEM, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 19: 739 (1965).",
      "journal-title": "BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "crossref",
      "first-page": "1647",
      "DOI": "10.1016/S0021-9258(18)97483-1",
      "volume": "240",
      "year": "1965",
      "unstructured": "MOORE, B.W., CHROMATOGRAPHIC AND ELECTROPHORECTIC FRACTIONATION OF SOLUBLE PROTEINS OF BRAIN AND LIVER, JOURNAL OF BIOLOGICAL CHEMISTRY 240: 1647 (1965).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.161.3839.370",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        11
      ]
    ],
    "date-time": "2024-01-11T09:06:50Z",
    "timestamp": 1704964010000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.161.3839.370"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1968,
        7,
        26
      ]
    ]
  },
  "references-count": 11,
  "journal-issue": {
    "issue": "3839",
    "published-print": {
      "date-parts": [
        [
          1968,
          7,
          26
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.161.3839.370"
  ],
  "URL": "http://dx.doi.org/10.1126/science.161.3839.370",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1968,
        7,
        26
      ]
    ]
  }
}