DOI: 10.1126/science.160.3829.774. Mitotic Synchrony in Mammalian Cells Treated with Nitrous Oxide at High Pressure

Mitotic Synchrony in Mammalian Cells Treated with Nitrous Oxide at High Pressure

10.1126/science.160.3829.774

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Mammalian cells grown in suspension or monolayer cultures were synchronized for cell division by the application of nitrous oxide under pressure. The metaphase block induced by nitrous oxide was dependent on pressure and was reversible. Exposure of HeLa cells to nitrous oxide had no significant effect on the synthesis of DNA, RNA, or protein. The progress of cells through the mitotic cycle was also unaffected. A high degree of mitotic synchrony was obtained in suspension cultures of HeLa cells treated with thymidine during exponential growth, resuspended in fresh medium, and then exposed to nitrous oxide.

Bibliography

Rao, P. N. (1968). Mitotic Synchrony in Mammalian Cells Treated with Nitrous Oxide at High Pressure. Science, 160(3829), 774â776.

Authors 1

Potu N. Rao (first)

References 19 Referenced 89

BOOTSMA, D, STUDIES ON SYNCHRONOUS DIVISION OF TISSUE CULTURE CELLS INITIATED BY EXCESS THYMIDINE, EXPERIMENTAL CELL RESEARCH 33: 301 (1964). (10.1016/S0014-4827(64)81035-1) / EXPERIMENTAL CELL RESEARCH (1964)
FERGUSON, J, C-MITOTIC ACTION OF SOME SIMPLE GASES, NATURE 165: 1021 (1950). / NATURE (1950)
FIRKET, H, EXP CELL RES 45: 11 (1966). / EXP CELL RES (1966)
MUELLER, G. S., EXPERIMENTAL CELL RESEARCH: 144 (1963). / EXPERIMENTAL CELL RESEARCH (1963)
NEWTON, A.A., PARASYNCHRONOUS DIVISION OF HELA CELLS, EXPERIMENTAL CELL RESEARCH 16: 624 (1959). (10.1016/0014-4827(59)90130-2) / EXPERIMENTAL CELL RESEARCH (1959)
OSTERGREN, G, Colchicine mitosis, chromosome contraction, narcosis and protein chain folding, HEREDITAS 30: 429 (1944). / HEREDITAS (1944)
PETERSEN, D.F., QUANTITY PRODUCTION OF SYNCHRONIZED MAMMALIAN CELLS IN SUSPENSION CULTURE, NATURE 203: 642 (1964). (10.1038/203642a0) / NATURE (1964)
PUCK, T.T., PHASING MITOTIC DELAY + CHROMOSOMAL ABERRATIONS IN MAMMALIAN CELLS, SCIENCE 144: 565 (1964). / SCIENCE (1964)
Rao, P. N., Cell Synchrony-Studies in Biosynthetic Regulation: 332 (1966). / Cell Synchrony-Studies in Biosynthetic Regulation (1966)
RAO, P.N., MITOTIC NON-DISJUNCTION OF SISTER CHROMATIDS AND ANOMALOUS MITOSIS INDUCED BY LOW TEMPERATURES IN HELA CELLS, EXPERIMENTAL CELL RESEARCH 43: 332 (1966). (10.1016/0014-4827(66)90060-7) / EXPERIMENTAL CELL RESEARCH (1966)
RAO, P.N., STRUCTURAL SPECIFICITY OF ESTROGENS IN INDUCTION OF MITOTIC CHROMATOD NON-DISJUNCTION IN HELA CELLS, EXPERIMENTAL CELL RESEARCH 48: 71 (1967). (10.1016/0014-4827(67)90277-7) / EXPERIMENTAL CELL RESEARCH (1967)
10.1126/science.148.3673.1092
10.1126/science.144.3622.1152
RUECKERT, R.R., STUDIES ON UNBALANCED GROWTH IN TISSUE CULTURE .1. INDUCTION AND CONSEQUENCES OF THYMIDINE DEFICIENCY, CANCER RESEARCH 20: 1584 (1960). / CANCER RESEARCH (1960)
SINCLAIR, W.K., VARIATIONS IN X-RAY RESPONSE DURING DIVISION CYCLE OF PARTIALLY SYNCHRONIZED CHINESE HAMSTER CELLS IN CULTURE, NATURE 199: 1158 (1963). (10.1038/1991158a0) / NATURE (1963)
STUBBLEFIELD, E, SYNCHRONIZATION OF CHINESE HAMSTER CELLS BY REVERSAL OF COLCEMID INHIBITION, EXPERIMENTAL CELL RESEARCH 40: 660 (1965). (10.1016/0014-4827(65)90244-2) / EXPERIMENTAL CELL RESEARCH (1965)
TERASIMA, T, GROWTH AND NUCLEIC ACID SYNTHESIS IN SYNCHRONOUSLY DIVIDING POPULATIONS OF HELA CELLS, EXPERIMENTAL CELL RESEARCH 30: 344 (1963). (10.1016/0014-4827(63)90306-9) / EXPERIMENTAL CELL RESEARCH (1963)
WHITFIELD, J.F., SYNCHRONISATION OF CELL DIVISION IN SUSPENSION CULTURES OF L STRAIN MOUSE CELLS, EXPERIMENTAL CELL RESEARCH 38: 208 (1965). (10.1016/0014-4827(65)90443-X) / EXPERIMENTAL CELL RESEARCH (1965)
XEROS, N, DEOXYRIBOSIDE CONTROL AND SYNCHRONIZATION OF MITOSIS, NATURE 194: 682 (1962). (10.1038/194682a0) / NATURE (1962)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:50 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 5:22 a.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 7:12 a.m.)
Issued	57 years, 3 months ago (May 17, 1968)
Published	57 years, 3 months ago (May 17, 1968)
Published Print	57 years, 3 months ago (May 17, 1968)

Funders 0

None

BibTeX

@article{Rao_1968, title={Mitotic Synchrony in Mammalian Cells Treated with Nitrous Oxide at High Pressure}, volume={160}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.160.3829.774}, DOI={10.1126/science.160.3829.774}, number={3829}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Rao, Potu N.}, year={1968}, month=may, pages={774–776} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        7,
        10
      ]
    ],
    "date-time": "2024-07-10T11:12:48Z",
    "timestamp": 1720609968013
  },
  "reference-count": 19,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "3829",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1968,
        5,
        17
      ]
    ]
  },
  "abstract": "<jats:p>Mammalian cells grown in suspension or monolayer cultures were synchronized for cell division by the application of nitrous oxide under pressure. The metaphase block induced by nitrous oxide was dependent on pressure and was reversible. Exposure of HeLa cells to nitrous oxide had no significant effect on the synthesis of DNA, RNA, or protein. The progress of cells through the mitotic cycle was also unaffected. A high degree of mitotic synchrony was obtained in suspension cultures of HeLa cells treated with thymidine during exponential growth, resuspended in fresh medium, and then exposed to nitrous oxide.</jats:p>",
  "DOI": "10.1126/science.160.3829.774",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        10,
        5
      ]
    ],
    "date-time": "2006-10-05T11:50:27Z",
    "timestamp": 1160049027000
  },
  "page": "774-776",
  "source": "Crossref",
  "is-referenced-by-count": 89,
  "title": "Mitotic Synchrony in Mammalian Cells Treated with Nitrous Oxide at High Pressure",
  "prefix": "10.1126",
  "volume": "160",
  "author": [
    {
      "given": "Potu N.",
      "family": "Rao",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Physiology and Biophysics, University of Kentucky Medical Center, Lexington"
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_2_1_1_1",
      "doi-asserted-by": "crossref",
      "first-page": "301",
      "DOI": "10.1016/S0014-4827(64)81035-1",
      "volume": "33",
      "year": "1964",
      "unstructured": "BOOTSMA, D, STUDIES ON SYNCHRONOUS DIVISION OF TISSUE CULTURE CELLS INITIATED BY EXCESS THYMIDINE, EXPERIMENTAL CELL RESEARCH 33: 301 (1964).",
      "journal-title": "EXPERIMENTAL CELL RESEARCH"
    },
    {
      "key": "e_1_2_1_2_1",
      "first-page": "1021",
      "volume": "165",
      "year": "1950",
      "unstructured": "FERGUSON, J, C-MITOTIC ACTION OF SOME SIMPLE GASES, NATURE 165: 1021 (1950).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_2_1_3_1",
      "first-page": "11",
      "volume": "45",
      "year": "1966",
      "unstructured": "FIRKET, H, EXP CELL RES 45: 11 (1966).",
      "journal-title": "EXP CELL RES"
    },
    {
      "key": "e_1_2_1_4_1",
      "first-page": "144",
      "year": "1963",
      "unstructured": "MUELLER, G. S., EXPERIMENTAL CELL RESEARCH: 144 (1963).",
      "journal-title": "EXPERIMENTAL CELL RESEARCH"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "crossref",
      "first-page": "624",
      "DOI": "10.1016/0014-4827(59)90130-2",
      "volume": "16",
      "year": "1959",
      "unstructured": "NEWTON, A.A., PARASYNCHRONOUS DIVISION OF HELA CELLS, EXPERIMENTAL CELL RESEARCH 16: 624 (1959).",
      "journal-title": "EXPERIMENTAL CELL RESEARCH"
    },
    {
      "key": "e_1_2_1_6_1",
      "first-page": "429",
      "volume": "30",
      "year": "1944",
      "unstructured": "OSTERGREN, G, Colchicine mitosis, chromosome contraction, narcosis and protein chain folding, HEREDITAS 30: 429 (1944).",
      "journal-title": "HEREDITAS"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "crossref",
      "first-page": "642",
      "DOI": "10.1038/203642a0",
      "volume": "203",
      "year": "1964",
      "unstructured": "PETERSEN, D.F., QUANTITY PRODUCTION OF SYNCHRONIZED MAMMALIAN CELLS IN SUSPENSION CULTURE, NATURE 203: 642 (1964).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_2_1_8_1",
      "first-page": "565",
      "volume": "144",
      "year": "1964",
      "unstructured": "PUCK, T.T., PHASING MITOTIC DELAY + CHROMOSOMAL ABERRATIONS IN MAMMALIAN CELLS, SCIENCE 144: 565 (1964).",
      "journal-title": "SCIENCE"
    },
    {
      "key": "e_1_2_1_9_1",
      "first-page": "332",
      "year": "1966",
      "unstructured": "Rao, P. N., Cell Synchrony-Studies in Biosynthetic Regulation: 332 (1966).",
      "journal-title": "Cell Synchrony-Studies in Biosynthetic Regulation"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "crossref",
      "first-page": "332",
      "DOI": "10.1016/0014-4827(66)90060-7",
      "volume": "43",
      "year": "1966",
      "unstructured": "RAO, P.N., MITOTIC NON-DISJUNCTION OF SISTER CHROMATIDS AND ANOMALOUS MITOSIS INDUCED BY LOW TEMPERATURES IN HELA CELLS, EXPERIMENTAL CELL RESEARCH 43: 332 (1966).",
      "journal-title": "EXPERIMENTAL CELL RESEARCH"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "crossref",
      "first-page": "71",
      "DOI": "10.1016/0014-4827(67)90277-7",
      "volume": "48",
      "year": "1967",
      "unstructured": "RAO, P.N., STRUCTURAL SPECIFICITY OF ESTROGENS IN INDUCTION OF MITOTIC CHROMATOD NON-DISJUNCTION IN HELA CELLS, EXPERIMENTAL CELL RESEARCH 48: 71 (1967).",
      "journal-title": "EXPERIMENTAL CELL RESEARCH"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.148.3673.1092"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.144.3622.1152"
    },
    {
      "key": "e_1_2_1_14_1",
      "first-page": "1584",
      "volume": "20",
      "year": "1960",
      "unstructured": "RUECKERT, R.R., STUDIES ON UNBALANCED GROWTH IN TISSUE CULTURE .1. INDUCTION AND CONSEQUENCES OF THYMIDINE DEFICIENCY, CANCER RESEARCH 20: 1584 (1960).",
      "journal-title": "CANCER RESEARCH"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "crossref",
      "first-page": "1158",
      "DOI": "10.1038/1991158a0",
      "volume": "199",
      "year": "1963",
      "unstructured": "SINCLAIR, W.K., VARIATIONS IN X-RAY RESPONSE DURING DIVISION CYCLE OF PARTIALLY SYNCHRONIZED CHINESE HAMSTER CELLS IN CULTURE, NATURE 199: 1158 (1963).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "crossref",
      "first-page": "660",
      "DOI": "10.1016/0014-4827(65)90244-2",
      "volume": "40",
      "year": "1965",
      "unstructured": "STUBBLEFIELD, E, SYNCHRONIZATION OF CHINESE HAMSTER CELLS BY REVERSAL OF COLCEMID INHIBITION, EXPERIMENTAL CELL RESEARCH 40: 660 (1965).",
      "journal-title": "EXPERIMENTAL CELL RESEARCH"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "crossref",
      "first-page": "344",
      "DOI": "10.1016/0014-4827(63)90306-9",
      "volume": "30",
      "year": "1963",
      "unstructured": "TERASIMA, T, GROWTH AND NUCLEIC ACID SYNTHESIS IN SYNCHRONOUSLY DIVIDING POPULATIONS OF HELA CELLS, EXPERIMENTAL CELL RESEARCH 30: 344 (1963).",
      "journal-title": "EXPERIMENTAL CELL RESEARCH"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "crossref",
      "first-page": "208",
      "DOI": "10.1016/0014-4827(65)90443-X",
      "volume": "38",
      "year": "1965",
      "unstructured": "WHITFIELD, J.F., SYNCHRONISATION OF CELL DIVISION IN SUSPENSION CULTURES OF L STRAIN MOUSE CELLS, EXPERIMENTAL CELL RESEARCH 38: 208 (1965).",
      "journal-title": "EXPERIMENTAL CELL RESEARCH"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "crossref",
      "first-page": "682",
      "DOI": "10.1038/194682a0",
      "volume": "194",
      "year": "1962",
      "unstructured": "XEROS, N, DEOXYRIBOSIDE CONTROL AND SYNCHRONIZATION OF MITOSIS, NATURE 194: 682 (1962).",
      "journal-title": "NATURE"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.160.3829.774",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        11
      ]
    ],
    "date-time": "2024-01-11T10:22:48Z",
    "timestamp": 1704968568000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.160.3829.774"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1968,
        5,
        17
      ]
    ]
  },
  "references-count": 19,
  "journal-issue": {
    "issue": "3829",
    "published-print": {
      "date-parts": [
        [
          1968,
          5,
          17
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.160.3829.774"
  ],
  "URL": "http://dx.doi.org/10.1126/science.160.3829.774",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1968,
        5,
        17
      ]
    ]
  }
}