DOI: 10.1126/science.1598575. High-Efficiency Expression and Solubilization of Functional T Cell Antigen Receptor Heterodimers

High-Efficiency Expression and Solubilization of Functional T Cell Antigen Receptor Heterodimers

10.1126/science.1598575

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The T cell receptor (TCR) ζ chain was attached to the TCR α and β extracellular domains to induce efficient expression of αβ heterodimers that can recognize complexes of antigen with major histocompatibility complex (MHC) molecules. Chimeric constructs expressed in RBL-2H3 cells were efficiently transported to the cell surface uniquely as disulfide-linked heterodimers. Transfectants were activated by specific antigen-MHC complexes, which demonstrated that the expressed αβ was functional and that CD3 was not required for antigen-MHC binding. Constructs with thrombin cleavage sites were efficiently cleaved to soluble disulfide-linked heterodimers. Thus, attachment of TCR ζ domains and protease cleavage sites to TCR α and β induces expression of demonstrably functional heterodimers that can be solubilized.

Bibliography

Engel, I., Ottenhoff, T. H. M., & Klausner, R. D. (1992). High-Efficiency Expression and Solubilization of Functional T Cell Antigen Receptor Heterodimers. Science, 256(5061), 1318â1321.

Authors 3

Isaac Engel (first)
Tom H. M. Ottenhoff (additional)
Richard D. Klausner (additional)

References 30 Referenced 57

BECKER, D.M., VARIABILITY AND REPERTOIRE SIZE OF T-CELL RECEPTOR V-ALPHA GENE SEGMENTS, NATURE 317: 430 (1985). (10.1038/317430a0) / NATURE (1985)
BENHAMOU, M, TYROSINE PHOSPHORYLATION COUPLED TO IGE RECEPTOR-MEDIATED SIGNAL TRANSDUCTION AND HISTAMINE-RELEASE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 87: 5327 (1990). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1990)
BONIFACINO, J.S., COLOCALIZED TRANSMEMBRANE DETERMINANTS FOR ER DEGRADATION AND SUBUNIT ASSEMBLY EXPLAIN THE INTRACELLULAR FATE OF TCR CHAINS, CELL 63: 503 (1990). (10.1016/0092-8674(90)90447-M) / CELL (1990)
10.1126/science.2294595
10.1126/science.2435001
CHEN, C, SELECTIVE DEGRADATION OF T-CELL ANTIGEN RECEPTOR CHAINS RETAINED IN A PRE-GOLGI COMPARTMENT, JOURNAL OF CELL BIOLOGY 107: 2149 (1988). (10.1083/jcb.107.6.2149) / JOURNAL OF CELL BIOLOGY (1988)
10.1038/309322a0
GREGOIRE, C, ENGINEERED SECRETED T-CELL RECEPTOR ALPHA-BETA HETERODIMERS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 8077 (1991). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1991)
HANSBURG, D, THE LYMPHOCYTE-T RESPONSE TO CYTOCHROME-C .4. DISTINGUISHABLE SITES ON A PEPTIDE ANTIGEN WHICH AFFECT ANTIGENIC STRENGTH AND MEMORY, JOURNAL OF IMMUNOLOGY 131: 319 (1983). (10.4049/jimmunol.131.1.319) / JOURNAL OF IMMUNOLOGY (1983)
HEDRICK, S.M., THE FINE SPECIFICITY OF ANTIGEN AND IA DETERMINANT RECOGNITION BY T-CELL HYBRIDOMA CLONES SPECIFIC FOR PIGEON CYTOCHROME-C, CELL 30: 141 (1982). (10.1016/0092-8674(82)90020-4) / CELL (1982)
HIGUCHI, R, A GENERAL-METHOD OF INVITRO PREPARATION AND SPECIFIC MUTAGENESIS OF DNA FRAGMENTS - STUDY OF PROTEIN AND DNA INTERACTIONS, NUCLEIC ACIDS RESEARCH 16: 7351 (1988). (10.1093/nar/16.15.7351) / NUCLEIC ACIDS RESEARCH (1988)
IRVING, B.A., THE CYTOPLASMIC DOMAIN OF THE T-CELL RECEPTOR ZETA-CHAIN IS SUFFICIENT TO COUPLE TO RECEPTOR-ASSOCIATED SIGNAL TRANSDUCTION PATHWAYS, CELL 64: 891 (1991). (10.1016/0092-8674(91)90314-O) / CELL (1991)
KLAUSNER, R.D., THE T-CELL ANTIGEN RECEPTOR - INSIGHTS INTO ORGANELLE BIOLOGY, ANNUAL REVIEW OF CELL BIOLOGY 6: 403 (1990). (10.1146/annurev.cb.06.110190.002155) / ANNUAL REVIEW OF CELL BIOLOGY (1990)
10.1146/annurev.iy.04.040186.002525
10.1038/227680a0
LETOURNEUR, F, T-CELL AND BASOPHIL ACTIVATION THROUGH THE CYTOPLASMIC TAIL OF T-CELL-RECEPTOR ZETA-FAMILY PROTEINS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 8905 (1991). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1991)
10.1126/science.1696397
METZGER, H, THE RECEPTOR WITH HIGH-AFFINITY FOR IMMUNOGLOBULIN-E, ANNUAL REVIEW OF IMMUNOLOGY 4: 419 (1986). (10.1146/annurev.iy.04.040186.002223) / ANNUAL REVIEW OF IMMUNOLOGY (1986)
NOVOTNY, J, A SOLUBLE, SINGLE-CHAIN T-CELL RECEPTOR FRAGMENT ENDOWED WITH ANTIGEN-COMBINING PROPERTIES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 8646 (1991). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1991)
ORLOFF, D.G., BIOCHEMICAL-CHARACTERIZATION OF THE ETA-CHAIN OF THE T-CELL RECEPTOR - A UNIQUE SUBUNIT RELATED TO ZETA, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 14812 (1989). (10.1016/S0021-9258(18)63772-X) / JOURNAL OF BIOLOGICAL CHEMISTRY (1989)
RACIOPPI, L, THE MOLECULAR-BASIS OF CLASS-II MHC ALLELIC CONTROL OF T-CELL RESPONSES, JOURNAL OF IMMUNOLOGY 147: 3718 (1991). (10.4049/jimmunol.147.11.3718) / JOURNAL OF IMMUNOLOGY (1991)
SAITO, T, SPECIFIC ANTIGEN IA ACTIVATION OF TRANSFECTED HUMAN T-CELLS EXPRESSING MURINE TI ALPHA-BETA-HUMAN T3 RECEPTOR COMPLEXES, NATURE 325: 125 (1987). (10.1038/325125a0) / NATURE (1987)
SAMELSON, L.E., IDENTIFICATION OF THE COMPONENTS OF THE MURINE T-CELL ANTIGEN RECEPTOR COMPLEX, CELL 43: 223 (1985). (10.1016/0092-8674(85)90027-3) / CELL (1985)
Samelson, L. E., Proceedings of the National Academy of Sciences of the United States of America 80: 6972 (1983). / Proceedings of the National Academy of Sciences of the United States of America (1983)
SLANETZ, A.E., HETERODIMERIC, DISULFIDE-LINKED ALPHA-BETA T-CELL RECEPTORS IN SOLUTION, EUROPEAN JOURNAL OF IMMUNOLOGY 21: 179 (1991). (10.1002/eji.1830210127) / EUROPEAN JOURNAL OF IMMUNOLOGY (1991)
SMITH, D.B., SINGLE-STEP PURIFICATION OF POLYPEPTIDES EXPRESSED IN ESCHERICHIA-COLI AS FUSIONS WITH GLUTATHIONE S-TRANSFERASE, GENE 67: 31 (1988). (10.1016/0378-1119(88)90005-4) / GENE (1988)
SUZUKI C.K. unpublished data.
TAKEBE, Y, SR-ALPHA PROMOTER - AN EFFICIENT AND VERSATILE MAMMALIAN CDNA EXPRESSION SYSTEM COMPOSED OF THE SIMIAN VIRUS-40 EARLY PROMOTER AND THE R-U5 SEGMENT OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-1 LONG TERMINAL REPEAT, MOLECULAR AND CELLULAR BIOLOGY 8: 466 (1988). / MOLECULAR AND CELLULAR BIOLOGY (1988)
10.1126/science.3278377
WILEMAN, T, RECOGNITION FOR DEGRADATION IN THE ENDOPLASMIC-RETICULUM AND LYSOSOMES PREVENTS THE TRANSPORT OF SINGLE TCR-BETA AND CD3-DELTA SUBUNITS OF THE T-CELL ANTIGEN RECEPTOR TO THE SURFACE OF CELLS, INTERNATIONAL IMMUNOLOGY 2: 743 (1990). (10.1093/intimm/2.8.743) / INTERNATIONAL IMMUNOLOGY (1990)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 3:07 a.m.)
Indexed	1 year ago (Aug. 7, 2024, 8:33 a.m.)
Issued	33 years, 2 months ago (May 29, 1992)
Published	33 years, 2 months ago (May 29, 1992)
Published Print	33 years, 2 months ago (May 29, 1992)

Funders 0

None

BibTeX

@article{Engel_1992, title={High-Efficiency Expression and Solubilization of Functional T Cell Antigen Receptor Heterodimers}, volume={256}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1598575}, DOI={10.1126/science.1598575}, number={5061}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Engel, Isaac and Ottenhoff, Tom H. M. and Klausner, Richard D.}, year={1992}, month=may, pages={1318–1321} }

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