DOI: 10.1126/science.1585178. Protein Solvation in Allosteric Regulation: A Water Effect on Hemoglobin

Protein Solvation in Allosteric Regulation: A Water Effect on Hemoglobin

10.1126/science.1585178

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The oxygen affinity of hemoglobin varies linearly with the chemical potential of water in the bathing medium, as seen from the osmotic effect of several neutral solutes, namely sucrose, stachyose, and two polyethyleneglycols (molecular weights of 150 and 400). The data, analyzed either by Wyman linkage equations or by Gibbs-Duhem relations, show that ∼60 extra water molecules bind to hemoglobin during the transition from the fully deoxygenated tense (T) state to the fully oxygenated relaxed (R) state. This number, independent of the nature of the solute, agrees with the difference in water-accessible surface areas previously computed for the two conformations. The work of solvation in allosteric regulation can no longer go unrecognized.

Bibliography

Colombo, M. F., Rau, D. C., & Parsegian, V. A. (1992). Protein Solvation in Allosteric Regulation: A Water Effect on Hemoglobin. Science, 256(5057), 655â659.

Authors 3

Marcio F. Colombo (first)
Donald C. Rau (additional)
V. Adrian Parsegian (additional)

References 28 Referenced 268

ACKERS, G.K., THE ENERGETICS OF LIGAND-LINKED SUBUNIT ASSEMBLY IN HEMOGLOBIN REQUIRE A 3RD ALLOSTERIC STRUCTURE, BIOPHYSICAL CHEMISTRY 37: 371 (1990). (10.1016/0301-4622(90)88036-R) / BIOPHYSICAL CHEMISTRY (1990)
ANUSIEM, ACI, SPECTRAL EVIDENCE FOR 2 FORMS OF ACID FERRIHEMOGLOBIN, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 175: 138 (1976). (10.1016/0003-9861(76)90492-6) / ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (1976)
ARAKAWA, T, STABILIZATION OF PROTEIN-STRUCTURE BY SUGARS, BIOCHEMISTRY 21: 6536 (1982). (10.1021/bi00268a033) / BIOCHEMISTRY (1982)
BALDWIN, J, HEMOGLOBIN - STRUCTURAL-CHANGES RELATED TO LIGAND-BINDING AND ITS ALLOSTERIC MECHANISM, JOURNAL OF MOLECULAR BIOLOGY 129: 175 (1979). (10.1016/0022-2836(79)90277-8) / JOURNAL OF MOLECULAR BIOLOGY (1979)
BLANK, M, A SURFACE FREE-ENERGY MODEL FOR PROTEIN-STRUCTURE IN SOLUTION - HEMOGLOBIN EQUILIBRIA, COLLOIDS AND SURFACES 1: 139 (1980). (10.1016/0166-6622(80)80001-1) / COLLOIDS AND SURFACES (1980)
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CHOTHIA, C, ROLE OF SUBUNIT INTERFACES IN ALLOSTERIC MECHANISM OF HEMOGLOBIN, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 73: 3793 (1976). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1976)
COLOMBO, M.F., HYDRATION-DEPENDENT CONFORMATIONAL STATES OF HEMOGLOBIN - EQUILIBRIUM AND KINETIC-BEHAVIOR, BIOPHYSICAL CHEMISTRY 36: 33 (1990). (10.1016/0301-4622(90)85004-P) / BIOPHYSICAL CHEMISTRY (1990)
COLOMBO M.F. unpublished data.
CORDONE, L, CONFORMATIONAL AND FUNCTIONAL-PROPERTIES OF HEMOGLOBIN IN PERTURBED SOLVENT - KINETICS OF O-2 RELEASE, BIOPOLYMERS 22: 1677 (1983). (10.1002/bip.360220706) / BIOPOLYMERS (1983)
DICERA, E, THERMODYNAMICS OF LOCAL LINKAGE EFFECTS - CONTRACTED PARTITION-FUNCTIONS AND THE ANALYSIS OF SITE-SPECIFIC ENERGETICS, BIOPHYSICAL CHEMISTRY 37: 147 (1990). (10.1016/0301-4622(90)88015-K) / BIOPHYSICAL CHEMISTRY (1990)
HAIRE, R.N., HEMOGLOBIN-FUNCTION IN THE WATER ETHYLENE-GLYCOL CO-SOLVENT SYSTEM - LINKAGE BETWEEN OXYGEN BINDING AND HYDRATION, BIOCHEMISTRY 22: 327 (1983). (10.1021/bi00271a015) / BIOCHEMISTRY (1983)
HOPFIELD, J.J., RELATION BETWEEN STRUCTURE, COOPERATIVITY AND SPECTRA IN A MODEL OF HEMOGLOBIN ACTION, JOURNAL OF MOLECULAR BIOLOGY 77: 207 (1973). (10.1016/0022-2836(73)90332-X) / JOURNAL OF MOLECULAR BIOLOGY (1973)
JANIN, J, REACTION PATHWAY FOR THE QUATERNARY STRUCTURE CHANGE IN HEMOGLOBIN, BIOPOLYMERS 24: 509 (1985). (10.1002/bip.360240307) / BIOPOLYMERS (1985)
KORNBLATT, J.A., A NONTRADITIONAL ROLE FOR WATER IN THE CYTOCHROME-C-OXIDASE REACTION, BIOCHEMISTRY 29: 9370 (1990). (10.1021/bi00492a010) / BIOCHEMISTRY (1990)
LESK, A.M., HEMOGLOBIN - THE SURFACE BURIED BETWEEN THE ALPHA-1-BETA-1 AND ALPHA-2-BETA-2 DIMERS IN THE DEOXY AND OXY STRUCTURES, JOURNAL OF MOLECULAR BIOLOGY 183: 267 (1985). (10.1016/0022-2836(85)90219-0) / JOURNAL OF MOLECULAR BIOLOGY (1985)
MILLS, F.C., OXYGENATION-LINKED SUBUNIT INTERACTIONS IN HUMAN HEMOGLOBIN - EXPERIMENTAL STUDIES ON CONCENTRATION-DEPENDENCE OF OXYGENATION CURVES, BIOCHEMISTRY 15: 5350 (1976). (10.1021/bi00669a023) / BIOCHEMISTRY (1976)
PARSEGIAN, V.A., OSMOTIC-STRESS FOR THE DIRECT MEASUREMENT OF INTERMOLECULAR FORCES, METHODS IN ENZYMOLOGY 127: 400 (1986). (10.1016/0076-6879(86)27032-9) / METHODS IN ENZYMOLOGY (1986)
Perutz M. Mechanism of Cooperativity and Allosteric Regulation in Proteins (1990).
PODGORNIK, R, THE ACTION OF INTERHELICAL FORCES ON THE ORGANIZATION OF DNA DOUBLE HELICES - FLUCTUATION-ENHANCED DECAY OF ELECTROSTATIC DOUBLE-LAYER AND HYDRATION FORCES, MACROMOLECULES 22: 1780 (1989). (10.1021/ma00194a048) / MACROMOLECULES (1989)
PROUTY, M.S., CHEMICAL-POTENTIAL MEASUREMENTS OF DEOXYHEMOGLOBIN S-POLYMERIZATION - DETERMINATION OF THE PHASE-DIAGRAM OF AN ASSEMBLING PROTEIN, JOURNAL OF MOLECULAR BIOLOGY 184: 517 (1985). (10.1016/0022-2836(85)90298-0) / JOURNAL OF MOLECULAR BIOLOGY (1985)
RAND, R. P., BIOCHIMICA ET BIOPHYSICA ACTA 988: 351 (1989). (10.1016/0304-4157(89)90010-5) / BIOCHIMICA ET BIOPHYSICA ACTA (1989)
10.1126/science.2144663
RIGGS, A, METHODS IN ENZYMOLOGY 76: 5 (1981). (10.1016/0076-6879(81)76111-1) / METHODS IN ENZYMOLOGY (1981)
10.1016/S0065-3233(08)60190-4
Wyman J. Binding and Unkage Functional Chemistry of Biological Macromolecules (1990).
ZIMMERBERG, J, SOLUTE INACCESSIBLE AQUEOUS VOLUME CHANGES DURING OPENING OF THE POTASSIUM CHANNEL OF THE SQUID GIANT-AXON, BIOPHYSICAL JOURNAL 57: 1049 (1990). (10.1016/S0006-3495(90)82623-0) / BIOPHYSICAL JOURNAL (1990)
ZIMMERBERG, J, POLYMER INACCESSIBLE VOLUME CHANGES DURING OPENING AND CLOSING OF A VOLTAGE-DEPENDENT IONIC CHANNEL, NATURE 323: 36 (1986). (10.1038/323036a0) / NATURE (1986)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 5:05 a.m.)
Indexed	4 days, 17 hours ago (Aug. 21, 2025, 12:28 p.m.)
Issued	33 years, 3 months ago (May 1, 1992)
Published	33 years, 3 months ago (May 1, 1992)
Published Print	33 years, 3 months ago (May 1, 1992)

Funders 0

None

BibTeX

@article{Colombo_1992, title={Protein Solvation in Allosteric Regulation: A Water Effect on Hemoglobin}, volume={256}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1585178}, DOI={10.1126/science.1585178}, number={5057}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Colombo, Marcio F. and Rau, Donald C. and Parsegian, V. Adrian}, year={1992}, month=may, pages={655–659} }

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    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1992,
        5
      ]
    ]
  }
}