DOI: 10.1126/science.158.3801.658. Polyribosomes of Growing Bacteria

10.1126/science.158.3801.658

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A method of extracting polyribosomes has been developed which uses lysozyme on bacteria in the exponential phase of growth. This procedure recovers more than 80 percent of the total ribonucleic acid and nearly all of the soluble protein. Analysis of the lysate on sucrose gradients shows that 80 to 90 percent of the ribosomes sediment with the polysome fractions, indicating little or no degradation. The method has been applied to both a Gram-positive and a Gram-negative organism under various growth conditions and has proved uniformly successful.

Bibliography

Flessel, C. P., Ralph, P., & Rich, A. (1967). Polyribosomes of Growing Bacteria. Science, 158(3801), 658â660.

Authors 3

C. Peter Flessel (first)
Peter Ralph (additional)
Alexander Rich (additional)

References 13 Referenced 74

10.1126/science.149.3684.647
Flessel C. in preparation.
GODSON, G.N., REPLICATION OF BACTERIOPHAGE MS2 .6. INTERACTION BETWEEN BACTERIOPHAGE RNA AND CELLULAR COMPONENTS IN MS2-INFECTED ESCHERICHIA COLI, JOURNAL OF MOLECULAR BIOLOGY 23: 495 (1967). (10.1016/S0022-2836(67)80121-9) / JOURNAL OF MOLECULAR BIOLOGY (1967)
GOLDSTEIN, A, JOURNAL OF MOLECULAR BIOLOGY 9: 213 (1964). (10.1016/S0022-2836(64)80102-9) / JOURNAL OF MOLECULAR BIOLOGY (1964)
GUTHRIE, G. D., JOURNAL OF MOLECULAR BIOLOGY 2: 297 (1960). (10.1016/S0022-2836(60)80026-5) / JOURNAL OF MOLECULAR BIOLOGY (1960)
HOTHAMIGLEWSKI, B, REPLICATION OF BACTERIOPHAGE RIBONUCLEIC ACID - ALTERATIONS IN POLYRIBOSOME PATTERNS AND ASSOCIATION OF DOUBLE-STRANDED RNA WITH POLYRIBOSOMES IN ESCHERICHIA COLI INFECTED WITH BACTERIOPHAGE R17, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 58: 743 (1967). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1967)
KIHO, Y, A POLYCISTRONIC MESSENGER RNA ASSOCIATED WITH BETA-GALACTOSIDASE INDUCTION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 54: 1751 (1965). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1965)
MANGIAROTTI, G, POLYRIBOSOME METABOLISM IN ESCHERICHIA COLI .1. EXTRACTION OF POLYRIBSOMES AND RIBOSOMAL SUBUNITS FROM FRAGILE GROWING ESCHERICHIA COLI, JOURNAL OF MOLECULAR BIOLOGY 20: 123 (1966). (10.1016/0022-2836(66)90122-7) / JOURNAL OF MOLECULAR BIOLOGY (1966)
Mitchell, P., Journal of General Microbiology 15: 512 (1956). (10.1099/00221287-15-3-512) / Journal of General Microbiology (1956)
MORRIS, M, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 56: 262 (1966). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1966)
PARDEE, A.B., GENETIC CONTROL AND CYTOPLASMIC EXPRESSION OF INDUCIBILITY IN THE SYNTHESIS OF BETA-GALACTOSIDASE BY E-COLI, JOURNAL OF MOLECULAR BIOLOGY 1: 165 (1959). (10.1016/S0022-2836(59)80045-0) / JOURNAL OF MOLECULAR BIOLOGY (1959)
10.1126/science.153.3740.1119
SCHAECHTER, M, SYNTHESIS OF MESSENGER RIBONUCLEIC ACID AND ASSEMBLY OF POLYSOMES IN BACILLUS MEGATERIUM INFECTED WITH BACTERIOPHAGE, JOURNAL OF MOLECULAR BIOLOGY 22: 223 (1966). (10.1016/0022-2836(66)90128-8) / JOURNAL OF MOLECULAR BIOLOGY (1966)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:08 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 3:21 a.m.)
Indexed	1 year, 7 months ago (Jan. 11, 2024, 7:03 p.m.)
Issued	57 years, 9 months ago (Nov. 3, 1967)
Published	57 years, 9 months ago (Nov. 3, 1967)
Published Print	57 years, 9 months ago (Nov. 3, 1967)

Funders 0

None

BibTeX

@article{Flessel_1967, title={Polyribosomes of Growing Bacteria}, volume={158}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.158.3801.658}, DOI={10.1126/science.158.3801.658}, number={3801}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Flessel, C. Peter and Ralph, Peter and Rich, Alexander}, year={1967}, month=nov, pages={658–660} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        1,
        12
      ]
    ],
    "date-time": "2024-01-12T00:03:29Z",
    "timestamp": 1705017809476
  },
  "reference-count": 13,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "3801",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1967,
        11,
        3
      ]
    ]
  },
  "abstract": "<jats:p>A method of extracting polyribosomes has been developed which uses lysozyme on bacteria in the exponential phase of growth. This procedure recovers more than 80 percent of the total ribonucleic acid and nearly all of the soluble protein. Analysis of the lysate on sucrose gradients shows that 80 to 90 percent of the ribosomes sediment with the polysome fractions, indicating little or no degradation. The method has been applied to both a Gram-positive and a Gram-negative organism under various growth conditions and has proved uniformly successful.</jats:p>",
  "DOI": "10.1126/science.158.3801.658",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        10,
        5
      ]
    ],
    "date-time": "2006-10-05T11:08:12Z",
    "timestamp": 1160046492000
  },
  "page": "658-660",
  "source": "Crossref",
  "is-referenced-by-count": 74,
  "title": "Polyribosomes of Growing Bacteria",
  "prefix": "10.1126",
  "volume": "158",
  "author": [
    {
      "given": "C. Peter",
      "family": "Flessel",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Biology, Massachusetts Institute of Technology, Cambridge 02139"
        }
      ]
    },
    {
      "given": "Peter",
      "family": "Ralph",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biology, Massachusetts Institute of Technology, Cambridge 02139"
        }
      ]
    },
    {
      "given": "Alexander",
      "family": "Rich",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biology, Massachusetts Institute of Technology, Cambridge 02139"
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_2_1_1_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.149.3684.647"
    },
    {
      "key": "e_1_2_1_2_1",
      "unstructured": "Flessel C. in preparation."
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "crossref",
      "first-page": "495",
      "DOI": "10.1016/S0022-2836(67)80121-9",
      "volume": "23",
      "year": "1967",
      "unstructured": "GODSON, G.N., REPLICATION OF BACTERIOPHAGE MS2 .6. INTERACTION BETWEEN BACTERIOPHAGE RNA AND CELLULAR COMPONENTS IN MS2-INFECTED ESCHERICHIA COLI, JOURNAL OF MOLECULAR BIOLOGY 23: 495 (1967).",
      "journal-title": "JOURNAL OF MOLECULAR BIOLOGY"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "crossref",
      "first-page": "213",
      "DOI": "10.1016/S0022-2836(64)80102-9",
      "volume": "9",
      "year": "1964",
      "unstructured": "GOLDSTEIN, A, JOURNAL OF MOLECULAR BIOLOGY 9: 213 (1964).",
      "journal-title": "JOURNAL OF MOLECULAR BIOLOGY"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "crossref",
      "first-page": "297",
      "DOI": "10.1016/S0022-2836(60)80026-5",
      "volume": "2",
      "year": "1960",
      "unstructured": "GUTHRIE, G. D., JOURNAL OF MOLECULAR BIOLOGY 2: 297 (1960).",
      "journal-title": "JOURNAL OF MOLECULAR BIOLOGY"
    },
    {
      "key": "e_1_2_1_6_1",
      "first-page": "743",
      "volume": "58",
      "year": "1967",
      "unstructured": "HOTHAMIGLEWSKI, B, REPLICATION OF BACTERIOPHAGE RIBONUCLEIC ACID - ALTERATIONS IN POLYRIBOSOME PATTERNS AND ASSOCIATION OF DOUBLE-STRANDED RNA WITH POLYRIBOSOMES IN ESCHERICHIA COLI INFECTED WITH BACTERIOPHAGE R17, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 58: 743 (1967).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_7_1",
      "first-page": "1751",
      "volume": "54",
      "year": "1965",
      "unstructured": "KIHO, Y, A POLYCISTRONIC MESSENGER RNA ASSOCIATED WITH BETA-GALACTOSIDASE INDUCTION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 54: 1751 (1965).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "crossref",
      "first-page": "123",
      "DOI": "10.1016/0022-2836(66)90122-7",
      "volume": "20",
      "year": "1966",
      "unstructured": "MANGIAROTTI, G, POLYRIBOSOME METABOLISM IN ESCHERICHIA COLI .1. EXTRACTION OF POLYRIBSOMES AND RIBOSOMAL SUBUNITS FROM FRAGILE GROWING ESCHERICHIA COLI, JOURNAL OF MOLECULAR BIOLOGY 20: 123 (1966).",
      "journal-title": "JOURNAL OF MOLECULAR BIOLOGY"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "crossref",
      "first-page": "512",
      "DOI": "10.1099/00221287-15-3-512",
      "volume": "15",
      "year": "1956",
      "unstructured": "Mitchell, P., Journal of General Microbiology 15: 512 (1956).",
      "journal-title": "Journal of General Microbiology"
    },
    {
      "key": "e_1_2_1_10_1",
      "first-page": "262",
      "volume": "56",
      "year": "1966",
      "unstructured": "MORRIS, M, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 56: 262 (1966).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "crossref",
      "first-page": "165",
      "DOI": "10.1016/S0022-2836(59)80045-0",
      "volume": "1",
      "year": "1959",
      "unstructured": "PARDEE, A.B., GENETIC CONTROL AND CYTOPLASMIC EXPRESSION OF INDUCIBILITY IN THE SYNTHESIS OF BETA-GALACTOSIDASE BY E-COLI, JOURNAL OF MOLECULAR BIOLOGY 1: 165 (1959).",
      "journal-title": "JOURNAL OF MOLECULAR BIOLOGY"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.153.3740.1119"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "crossref",
      "first-page": "223",
      "DOI": "10.1016/0022-2836(66)90128-8",
      "volume": "22",
      "year": "1966",
      "unstructured": "SCHAECHTER, M, SYNTHESIS OF MESSENGER RIBONUCLEIC ACID AND ASSEMBLY OF POLYSOMES IN BACILLUS MEGATERIUM INFECTED WITH BACTERIOPHAGE, JOURNAL OF MOLECULAR BIOLOGY 22: 223 (1966).",
      "journal-title": "JOURNAL OF MOLECULAR BIOLOGY"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.158.3801.658",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        11
      ]
    ],
    "date-time": "2024-01-11T08:21:16Z",
    "timestamp": 1704961276000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.158.3801.658"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1967,
        11,
        3
      ]
    ]
  },
  "references-count": 13,
  "journal-issue": {
    "issue": "3801",
    "published-print": {
      "date-parts": [
        [
          1967,
          11,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.158.3801.658"
  ],
  "URL": "http://dx.doi.org/10.1126/science.158.3801.658",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1967,
        11,
        3
      ]
    ]
  }
}