DOI: 10.1126/science.158.3801.655. Fracture Planes in an Ice-Bilayer Model Membrane System

Fracture Planes in an Ice-Bilayer Model Membrane System

10.1126/science.158.3801.655

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Experiments with transferred stearate layers were performed to determine the location of fracture planes in frozen ice-lipid systems. Bilayers and multilayers of carbon-14-labeled stearate were frozen in contact with an aqueous phase and then fractured. The distribution of radioactivity on both sides of the fracture showed that the stearate layers were cleaved apart predominantly in the plane of their hydrocarbon tails. Because bilayers split in this manner, it was possible to measure time-dependent exchange of label between the layers. Exchange occurred with a half-time of 50 minutes in the presence of calcium and 25 minutes in the absence of calcium. Since stearate bilayers and multilayers are models of hydrophobically stabilized structures, the strong influence of their hydrophobic region on the fracture plane provides an explanation of how the freeze-etch technique of electron microscopy can expose inner, hydrophobic faces of cell membranes.

Bibliography

Deamer, D. W., & Branton, D. (1967). Fracture Planes in an Ice-Bilayer Model Membrane System. Science, 158(3801), 655â657.

Authors 2

David W. Deamer (first)
Daniel Branton (additional)

References 20 Referenced 143

BAGG, J, COMPOSITION OF STEARIC ACID MONOLAYERS FROM CALCIUM-CONTAINING SUBSTRATES, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 86: 2759 (1964). (10.1021/ja01068a001) / JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (1964)
BLODGETT, K.B., Films built by depositing successive monomolecular layers on a solid surface, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 57: 1007 (1935). (10.1021/ja01309a011) / JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (1935)
BRANTON, D, FRACTURE FACES OF FROZEN MYELIN, EXPERIMENTAL CELL RESEARCH 45: 703 (1967). (10.1016/0014-4827(67)90175-9) / EXPERIMENTAL CELL RESEARCH (1967)
Branton, D., Experimental Cell Research 47: 648 (1967). (10.1016/0014-4827(67)90028-6) / Experimental Cell Research (1967)
BRANTON, D, SUBUNITS IN CHLOROPLAST LAMELLAE, JOURNAL OF ULTRASTRUCTURE RESEARCH 19: 283 (1967). (10.1016/S0022-5320(67)80222-3) / JOURNAL OF ULTRASTRUCTURE RESEARCH (1967)
BRANTON, D, FRACTURE FACES OF FROZEN MEMBRANES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 55: 1048 (1966). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1966)
BYRNE, P, LIQUID CRYSTALLINE NATURE OF PHOSPHOLIPIDS, NATURE 202: 987 (1964). (10.1038/202987a0) / NATURE (1964)
Cornwell, D. G., Proteins and Their Reactions: 117 (1964). / Proteins and Their Reactions (1964)
DEAMER, D.W., CALCIUM ACTION ON FATTY ACID AND PHOSPHOLIPID MONOLAYERS AND ITS RELATION TO CELL MEMBRANE, BIOCHIMICA ET BIOPHYSICA ACTA 116: 555 (1966). (10.1016/0005-2760(66)90125-1) / BIOCHIMICA ET BIOPHYSICA ACTA (1966)
DEAMER, D.W., PROPERTIES COMPOSITION AND STRUCTURE OF STEARIC ACID-STEARATE MONOLAYERS ON ALKALINE EARTH SOLUTIONS, JOURNAL OF LIPID RESEARCH 8: 255 (1967). (10.1016/S0022-2275(20)40146-4) / JOURNAL OF LIPID RESEARCH (1967)
Greaves, R. I. N., Biological Applications of Freezing and Drying: 125 (1954). / Biological Applications of Freezing and Drying (1954)
KAUZMANN, W, SOME FACTORS IN THE INTERPRETATION OF PROTEIN DENATURATION, ADVANCES IN PROTEIN CHEMISTRY 14: 1 (1959). (10.1016/S0065-3233(08)60608-7) / ADVANCES IN PROTEIN CHEMISTRY (1959)
KIMIZUKA, H, BINDING OF CALCIUM ION TO LECITHIN FILM, NATURE 196: 995 (1962). / NATURE (1962)
10.1126/science.87.2266.493
MOOR, H, JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY 10: 1 (1961). / JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY (1961)
MOOR, H, FINE STRUCTURE IN FROZEN-ETCHED YEAST CELLS, JOURNAL OF CELL BIOLOGY 17: 609 (1963). (10.1083/jcb.17.3.609) / JOURNAL OF CELL BIOLOGY (1963)
NEMETHY, G, STRUCTURE OF WATER AND OF AQUEOUS SOLUTIONS, CRYOBIOLOGY 3: 19 (1966). (10.1016/S0011-2240(66)80146-3) / CRYOBIOLOGY (1966)
ROTHEN, A, ENZYMATIC REACTION ACROSS A THIN MEMBRANE, JOURNAL OF PHYSICAL CHEMISTRY 63: 1929 (1959). (10.1021/j150581a032) / JOURNAL OF PHYSICAL CHEMISTRY (1959)
SALEM, L, ROLE OF LONG-RANGE FORCES IN COHESION OF LIPOPROTEINS, CANADIAN JOURNAL OF BIOCHEMISTRY AND PHYSIOLOGY 40: 1287 (1962). (10.1139/y62-145) / CANADIAN JOURNAL OF BIOCHEMISTRY AND PHYSIOLOGY (1962)
STEERE, R.L.,JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY 3: 45 (1957). (10.1083/jcb.3.1.45) / JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY (1957)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:08 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 3:21 a.m.)
Indexed	11 months, 2 weeks ago (Sept. 9, 2024, 4:39 p.m.)
Issued	57 years, 9 months ago (Nov. 3, 1967)
Published	57 years, 9 months ago (Nov. 3, 1967)
Published Print	57 years, 9 months ago (Nov. 3, 1967)

Funders 0

None

BibTeX

@article{Deamer_1967, title={Fracture Planes in an Ice-Bilayer Model Membrane System}, volume={158}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.158.3801.655}, DOI={10.1126/science.158.3801.655}, number={3801}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Deamer, David W. and Branton, Daniel}, year={1967}, month=nov, pages={655–657} }

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