Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Positive control of the sex-specific alternative splicing of doublesex ( dsx ) precursor messenger RNA (pre-mRNA) in Drosophila melanogaster involves the activation of a female-specific 3′ splice site by the products of the transformer ( tra ) and transformer-2 ( tra-2 ) genes. The mechanisms of this process were investigated in an in vitro system in which the female-specific 3′ splice site could be activated by recombinant Tra or Tra-2 (or both). An exon sequence essential for regulation in vivo was shown to be both necessary and sufficient for activation in vitro. Nuclear proteins in addition to Tra and Tra-2 were found to bind specifically to this exon sequence. Therefore, Tra and Tra-2 may act by promoting the assembly of a multiprotein complex on the exon sequence. This complex may facilitate recognition of the adjacent 3′ splice site by the splicing machinery.

Bibliography

Tian, M., & Maniatis, T. (1992). Positive Control of Pre-mRNA Splicing in Vitro. Science, 256(5054), 237–240.

Authors 2
  1. Ming Tian (first)
  2. Tom Maniatis (additional)
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Dates
Type When
Created 18 years, 10 months ago (Oct. 5, 2006, 7:03 p.m.)
Deposited 1 year, 7 months ago (Jan. 11, 2024, 5:04 a.m.)
Indexed 2 weeks, 5 days ago (Aug. 6, 2025, 9:50 a.m.)
Issued 33 years, 4 months ago (April 10, 1992)
Published 33 years, 4 months ago (April 10, 1992)
Published Print 33 years, 4 months ago (April 10, 1992)
Funders 0

None

@article{Tian_1992, title={Positive Control of Pre-mRNA Splicing in Vitro}, volume={256}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1566072}, DOI={10.1126/science.1566072}, number={5054}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Tian, Ming and Maniatis, Tom}, year={1992}, month=apr, pages={237–240} }