DOI: 10.1126/science.156.3774.525. Turnover of Rat Liver Tyrosine Transaminase: Stabilization after Inhibition of Protein Synthesis

Turnover of Rat Liver Tyrosine Transaminase: Stabilization after Inhibition of Protein Synthesis

10.1126/science.156.3774.525

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Turnover of the rat liver tyrosine transaminase in vivo was measured by a label and chase procedure under conditions where the amount of enzyme undergoes no change. Half-life of the 14 C-labeled enzyme in this basal condition was found to be 1.5 ± 0.3 hours. Inhibitors of protein synthesis (cycloheximide or puromycin) do not appreciably influence the basal enzyme level over a 5-hour period, although these drugs will block hormonal induction of this enzyme. In pulse-labeling experiments, cycloheximide blocked transaminase synthesis almost completely. The conclusion that enzyme degradation, as well as synthesis, must be blocked when protein synthesis is stopped was confirmed in experiments showing that labeled enzyme is stable in the liver of rats treated with cycloheximide. The participation of a continuously synthesized polypeptide in the degradative phase of transaminase turnover is suggested.

Bibliography

Kenney, F. T. (1967). Turnover of Rat Liver Tyrosine Transaminase: Stabilization after Inhibition of Protein Synthesis. Science, 156(3774), 525â528.

Authors 1

Francis T. Kenney (first)

References 19 Referenced 220

BECHET, J, INDICATION OF A SPECIFIC REGULATORY BINDING PROTEIN FOR ORNITHINETRANSCARBAMYLASE IN SACCHAROMYCES CEREVISIAE, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 21: 226 (1965). (10.1016/0006-291X(65)90276-7) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1965)
BERLIN, C.M., INFLUENCE OF TURNOVER RATES ON RESPONSES OF ENZYMES TO CORTISONE, MOLECULAR PHARMACOLOGY 1: 149 (1965). / MOLECULAR PHARMACOLOGY (1965)
GARREN, L.D., PARADOXICAL EFFECT OF ACTINOMYCIN D - MECHANISM OF REGULATION OF ENZYME SYNTHESIS BY HYDROCORTISONE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 52: 1121 (1964). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1964)
GORNALL, A.G., DETERMINATION OF SERUM PROTEINS BY MEANS OF THE BIURET REACTION, JOURNAL OF BIOLOGICAL CHEMISTRY 177: 751 (1949). (10.1016/S0021-9258(18)57021-6) / JOURNAL OF BIOLOGICAL CHEMISTRY (1949)
GREENGARD, O, RELATION OF CORTISONE AND SYNTHESIS OF RIBONUCLEIC ACID TO INDUCED AND DEVELOPMENTAL ENZYME FORMATION, JOURNAL OF BIOLOGICAL CHEMISTRY 238: 1548 (1963). (10.1016/S0021-9258(18)81220-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1963)
GROSSMAN, A, FEDERATION PROCEEDINGS 25: 285 (1966). / FEDERATION PROCEEDINGS (1966)
Holten D. in preparation.
KENNEY, F.T., PROPERTIES OF PARTIALLY PURIFIED TYROSINE-ALPHA-KETOGLUTARATE TRANSAMINASE FROM RAT LIVER, JOURNAL OF BIOLOGICAL CHEMISTRY 234: 2707 (1959). (10.1016/S0021-9258(18)69764-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1959)
KENNEY, F.T., INDUCTION OF TYROSINE-ALPHA-KETOGLUTARATE TRANSAMINASE IN RAT LIVER .3. IMMUNOCHEMICAL ANALYSIS, JOURNAL OF BIOLOGICAL CHEMISTRY 237: 1610 (1962). (10.1016/S0021-9258(19)83749-3) / JOURNAL OF BIOLOGICAL CHEMISTRY (1962)
KENNEY, F.T., INDUCTION OF TYROSINE-ALPHA-KETOGLUTARATE TRANSAMINASE IN RAT LIVER .4. EVIDENCE FOR AN INCREASE IN RATE OF ENZYME SYNTHESIS, JOURNAL OF BIOLOGICAL CHEMISTRY 237: 3495 (1962). (10.1016/S0021-9258(19)70845-X) / JOURNAL OF BIOLOGICAL CHEMISTRY (1962)
KENNEY, F.T., REPRESSION OF ENZYME SYNTHESIS AT TRANSLATIONAL LEVEL AND ITS HORMONAL CONTROL, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 54: 1693 (1965). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1965)
LIN, E.C., SPECIFICITY OF THE ADAPTIVE RESPONSE OF TYROSINE-ALPHA-KETOGLUTARATE TRANSAMINASE IN THE RAT, JOURNAL OF BIOLOGICAL CHEMISTRY 233: 1186 (1958). (10.1016/S0021-9258(19)77365-7) / JOURNAL OF BIOLOGICAL CHEMISTRY (1958)
10.1016/0003-9861(61)90009-1
ROSEN, F, FEDERATION PROCEEDINGS 25: 285 (1966). / FEDERATION PROCEEDINGS (1966)
10.1126/science.146.3644.661
SCHIMKE, R.T., TURNOVER OF RAT LIVER ARGINASE, ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 102: 587 (1963). (10.1111/j.1749-6632.1963.tb13662.x) / ANNALS OF THE NEW YORK ACADEMY OF SCIENCES (1963)
SCHIMKE, R.T., ROLES OF SYNTHESIS AND DEGRADATION IN CONTROL OF RAT LIVER TRYPTOPHAN PYRROLASE, JOURNAL OF BIOLOGICAL CHEMISTRY 240: 322 (1965). (10.1016/S0021-9258(18)97652-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1965)
SUSSMAN, M, INHIBITION BY ACTIDIONE OF PROTEIN SYNTHESIS AND UDP-GAL POLYSACCHARIDE TRANSFERASE ACCUMULATION IN DICTYOSTELIUM DISCOIDEUM, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 18: 763 (1965). (10.1016/0006-291X(65)90851-X) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1965)
TSCHUDY, D.P., A MODEL FOR CALCULATING MESSENGER RNA HALF-LIFE - SHORT LIVED MESSENGER RNA IN INDUCTION OF MAMMALIAN DELTA-AMINOLEVULINIC ACID SYNTHETASE, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 21: 480 (1965). (10.1016/0006-291X(65)90408-0) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1965)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:08 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 3:09 a.m.)
Indexed	1 year, 7 months ago (Jan. 11, 2024, 7:04 p.m.)
Issued	58 years, 4 months ago (April 28, 1967)
Published	58 years, 4 months ago (April 28, 1967)
Published Print	58 years, 4 months ago (April 28, 1967)

Funders 0

None

BibTeX

@article{Kenney_1967, title={Turnover of Rat Liver Tyrosine Transaminase: Stabilization after Inhibition of Protein Synthesis}, volume={156}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.156.3774.525}, DOI={10.1126/science.156.3774.525}, number={3774}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Kenney, Francis T.}, year={1967}, month=apr, pages={525–528} }

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