DOI: 10.1126/science.1549777. Actin-Binding Protein Requirement for Cortical Stability and Efficient Locomotion

Actin-Binding Protein Requirement for Cortical Stability and Efficient Locomotion

10.1126/science.1549777

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Three unrelated tumor cell lines derived from human malignant melanomas lack actin-binding protein (ABP), which cross-links actin filaments in vitro and connects these filaments to plasma membrane glycoproteins. The ABP-deficient cells have impaired locomotion and display circumferential blebbing of the plasma membrane. Expression of ABP in one of the lines after transfection restored translocational motility and reduced membrane blebbing. These findings establish that ABP functions to stabilize cortical actin in vivo and is required for efficient cell locomotion.

Bibliography

Cunningham, C. C., Gorlin, J. B., Kwiatkowski, D. J., Hartwig, J. H., Janmey, P. A., Byers, H. R., & Stossel, T. P. (1992). Actin-Binding Protein Requirement for Cortical Stability and Efficient Locomotion. Science, 255(5042), 325â327.

Authors 7

C. Casey Cunningham (first)
Jed B. Gorlin (additional)
David J. Kwiatkowski (additional)
John H. Hartwig (additional)
Paul A. Janmey (additional)
H. Randolph Byers (additional)
Thomas P. Stossel (additional)

References 30 Referenced 480

BRINK, M, A DICTYOSTELIUM MUTANT LACKING AN F-ACTIN CROSS-LINKING PROTEIN, THE 120-KD GELATION FACTOR, JOURNAL OF CELL BIOLOGY 111: 1477 (1990). (10.1083/jcb.111.4.1477) / JOURNAL OF CELL BIOLOGY (1990)
BROTSCHI, E.A., GELATION OF ACTIN BY ACTIN-BINDING PROTEIN, JOURNAL OF BIOLOGICAL CHEMISTRY 253: 8988 (1978). (10.1016/S0021-9258(17)34275-8) / JOURNAL OF BIOLOGICAL CHEMISTRY (1978)
BYERS, H.R., CELL-MIGRATION AND ACTIN ORGANIZATION IN CULTURED HUMAN PRIMARY, RECURRENT CUTANEOUS AND METASTATIC MELANOMA - TIME-LAPSE AND IMAGE-ANALYSIS, AMERICAN JOURNAL OF PATHOLOGY 139: 423 (1991). / AMERICAN JOURNAL OF PATHOLOGY (1991)
10.1021/bi00591a005
DAVIES, W.A., PERIPHERAL HYALINE BLEBS (PODOSOMES) OF MACROPHAGES, JOURNAL OF CELL BIOLOGY 75: 941 (1977). (10.1083/jcb.75.3.941) / JOURNAL OF CELL BIOLOGY (1977)
Eicke, H.-F., Physical Networks: Polymers and Gels: 169 (1990). / Physical Networks: Polymers and Gels (1990)
EZZELL, R.M., LOCALIZATION OF THE DOMAIN OF ACTIN-BINDING PROTEIN THAT BINDS TO MEMBRANE GLYCOPROTEIN-IB AND ACTIN IN HUMAN-PLATELETS, JOURNAL OF BIOLOGICAL CHEMISTRY 263: 13303 (1988). (10.1016/S0021-9258(18)37705-6) / JOURNAL OF BIOLOGICAL CHEMISTRY (1988)
10.1016/0003-2697(83)90418-9
FOX, JEB, IDENTIFICATION OF ACTIN-BINDING PROTEIN AS THE PROTEIN LINKING THE MEMBRANE SKELETON TO GLYCOPROTEINS ON PLATELET PLASMA-MEMBRANES, JOURNAL OF BIOLOGICAL CHEMISTRY 260: 11970 (1985). (10.1016/S0021-9258(17)38972-X) / JOURNAL OF BIOLOGICAL CHEMISTRY (1985)
GORLIN, J.B., HUMAN ENDOTHELIAL ACTIN-BINDING PROTEIN (ABP-280, NONMUSCLE FILAMIN) - A MOLECULAR LEAF SPRING, JOURNAL OF CELL BIOLOGY 111: 1089 (1990). (10.1083/jcb.111.3.1089) / JOURNAL OF CELL BIOLOGY (1990)
GUNNING, P, A HUMAN BETA-ACTIN EXPRESSION VECTOR SYSTEM DIRECTS HIGH-LEVEL ACCUMULATION OF ANTISENSE TRANSCRIPTS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 84: 4831 (1987). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1987)
HARTWIG, J.H., ISOLATION AND PROPERTIES OF ACTIN, MYOSIN, AND A NEW ACTIN-BINDING PROTEIN IN RABBIT ALVEOLAR MACROPHAGES, JOURNAL OF BIOLOGICAL CHEMISTRY 250: 5696 (1975). (10.1016/S0021-9258(19)41235-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1975)
HARTWIG, J.H., ACTIN-BINDING PROTEIN PROMOTES THE BIPOLAR AND PERPENDICULAR BRANCHING OF ACTIN-FILAMENTS, JOURNAL OF CELL BIOLOGY 87: 841 (1980). (10.1083/jcb.87.3.841) / JOURNAL OF CELL BIOLOGY (1980)
HARTWIG, J.H., ASSOCIATION OF PROFILIN WITH FILAMENT-FREE REGIONS OF HUMAN-LEUKOCYTE AND PLATELET MEMBRANES AND REVERSIBLE MEMBRANE-BINDING DURING PLATELET ACTIVATION, JOURNAL OF CELL BIOLOGY 109: 1571 (1989). (10.1083/jcb.109.4.1571) / JOURNAL OF CELL BIOLOGY (1989)
Hartwig, J. H., The Lung: Scientific Foundation: 141 (1991). / The Lung: Scientific Foundation (1991)
JANMEY, P.A., A TORSION PENDULUM FOR MEASUREMENT OF THE VISCOELASTICITY OF BIOPOLYMERS AND ITS APPLICATION TO ACTIN NETWORKS, JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS 22: 41 (1991). (10.1016/0165-022X(91)90080-G) / JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS (1991)
KWIATKOWSKI, D.J., PREDOMINANT INDUCTION OF GELSOLIN AND ACTIN-BINDING PROTEIN DURING MYELOID DIFFERENTIATION, JOURNAL OF BIOLOGICAL CHEMISTRY 263: 13857 (1988). (10.1016/S0021-9258(18)68322-X) / JOURNAL OF BIOLOGICAL CHEMISTRY (1988)
LUBYPHELPS, K, THE SUBMICROSCOPIC PROPERTIES OF CYTOPLASM AS A DETERMINANT OF CELLULAR FUNCTION, ANNUAL REVIEW OF BIOPHYSICS AND BIOPHYSICAL CHEMISTRY 17: 369 (1988). (10.1146/annurev.bb.17.060188.002101) / ANNUAL REVIEW OF BIOPHYSICS AND BIOPHYSICAL CHEMISTRY (1988)
MESLAND, DAM, CYTOCHALASIN-B DISRUPTS THE ASSOCIATION OF FILAMENTOUS WEB AND PLASMA-MEMBRANE IN HEPATOCYTES, EXPERIMENTAL CELL RESEARCH 135: 431 (1981). (10.1016/0014-4827(81)90184-1) / EXPERIMENTAL CELL RESEARCH (1981)
MORROW, J, BIOCHIM BIOPHYS ACTA 830: 147 (1985). (10.1016/0167-4838(85)90022-6) / BIOCHIM BIOPHYS ACTA (1985)
NARVANEN, O, A MONOCLONAL-ANTIBODY AGAINST A SYNTHETIC PEPTIDE REVEALS COMMON STRUCTURES AMONG SPECTRINS AND ALPHA-ACTININ, FEBS LETTERS 224: 156 (1987). (10.1016/0014-5793(87)80440-4) / FEBS LETTERS (1987)
OHTA, Y, LIGAND-SENSITIVE BINDING OF ACTIN-BINDING PROTEIN TO IMMUNOGLOBULIN-G FC RECEPTOR-I (FC-GAMMA-RI), CELL 67: 275 (1991). (10.1016/0092-8674(91)90179-3) / CELL (1991)
OKITA, J.R., ON THE ASSOCIATION OF GLYCOPROTEIN 1B AND ACTIN-BINDING PROTEIN IN HUMAN-PLATELETS, JOURNAL OF CELL BIOLOGY 100: 317 (1985). (10.1083/jcb.100.1.317) / JOURNAL OF CELL BIOLOGY (1985)
POLLARD, T.D., ACTIN AND ACTIN-BINDING PROTEINS - A CRITICAL-EVALUATION OF MECHANISMS AND FUNCTIONS, ANNUAL REVIEW OF BIOCHEMISTRY 55: 987 (1986). (10.1146/annurev.bi.55.070186.005011) / ANNUAL REVIEW OF BIOCHEMISTRY (1986)
SCHLEICHER, M, A DICTYOSTELIUM MUTANT WITH SEVERE DEFECTS IN ALPHA-ACTININ - ITS CHARACTERIZATION USING CDNA PROBES AND MONOCLONAL-ANTIBODIES, JOURNAL OF CELL SCIENCE 90: 59 (1988). (10.1242/jcs.90.1.59) / JOURNAL OF CELL SCIENCE (1988)
Stossel, T. P., Cytomechanics: 131 (1987). / Cytomechanics (1987)
STOSSEL, T.P., FROM SIGNAL TO PSEUDOPOD - HOW CELLS CONTROL CYTOPLASMIC ACTIN ASSEMBLY, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 18261 (1989). (10.1016/S0021-9258(18)51454-X) / JOURNAL OF BIOLOGICAL CHEMISTRY (1989)
STOSSEL, T. P., JOURNAL OF CELL BIOLOGY 99: S15 (1984). (10.1083/jcb.99.1.15s) / JOURNAL OF CELL BIOLOGY (1984)
WANG, K, FILAMIN, A NEW HIGH-MOLECULAR-WEIGHT PROTEIN FOUND IN SMOOTH-MUSCLE AND NON-MUSCLE CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 72: 4483 (1975). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1975)
WEISS, E, ELECTRON-MICROSCOPY OF ISOLATED RAT HEPATOCYTES BEFORE AND AFTER TREATMENT WITH PHALLOIDIN, BEITRAGE ZUR PATHOLOGIE 150: 345 (1973). (10.1016/S0005-8165(73)80085-X) / BEITRAGE ZUR PATHOLOGIE (1973)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 4:25 a.m.)
Indexed	3 weeks, 3 days ago (Aug. 2, 2025, 1:20 a.m.)
Issued	33 years, 7 months ago (Jan. 17, 1992)
Published	33 years, 7 months ago (Jan. 17, 1992)
Published Print	33 years, 7 months ago (Jan. 17, 1992)

Funders 0

None

BibTeX

@article{Cunningham_1992, title={Actin-Binding Protein Requirement for Cortical Stability and Efficient Locomotion}, volume={255}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1549777}, DOI={10.1126/science.1549777}, number={5042}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Cunningham, C. Casey and Gorlin, Jed B. and Kwiatkowski, David J. and Hartwig, John H. and Janmey, Paul A. and Byers, H. Randolph and Stossel, Thomas P.}, year={1992}, month=jan, pages={325–327} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        2
      ]
    ],
    "date-time": "2025-08-02T05:20:00Z",
    "timestamp": 1754112000981
  },
  "reference-count": 30,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "5042",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1992,
        1,
        17
      ]
    ]
  },
  "abstract": "<jats:p>Three unrelated tumor cell lines derived from human malignant melanomas lack actin-binding protein (ABP), which cross-links actin filaments in vitro and connects these filaments to plasma membrane glycoproteins. The ABP-deficient cells have impaired locomotion and display circumferential blebbing of the plasma membrane. Expression of ABP in one of the lines after transfection restored translocational motility and reduced membrane blebbing. These findings establish that ABP functions to stabilize cortical actin in vivo and is required for efficient cell locomotion.</jats:p>",
  "DOI": "10.1126/science.1549777",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        10,
        5
      ]
    ],
    "date-time": "2006-10-05T23:03:06Z",
    "timestamp": 1160089386000
  },
  "page": "325-327",
  "source": "Crossref",
  "is-referenced-by-count": 480,
  "title": "Actin-Binding Protein Requirement for Cortical Stability and Efficient Locomotion",
  "prefix": "10.1126",
  "volume": "255",
  "author": [
    {
      "given": "C. Casey",
      "family": "Cunningham",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Divisions of Experimental Medicine and Hematology-Oncology, Brigham &amp; Women's Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115."
        }
      ]
    },
    {
      "given": "Jed B.",
      "family": "Gorlin",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Hematology-Oncology, Children's Hospital, Pediatric Oncology Division, Dana- Farber Cancer Institute, Boston, MA 02115."
        }
      ]
    },
    {
      "given": "David J.",
      "family": "Kwiatkowski",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Divisions of Experimental Medicine and Hematology-Oncology, Brigham &amp; Women's Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115."
        }
      ]
    },
    {
      "given": "John H.",
      "family": "Hartwig",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Divisions of Experimental Medicine and Hematology-Oncology, Brigham &amp; Women's Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115."
        }
      ]
    },
    {
      "given": "Paul A.",
      "family": "Janmey",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Divisions of Experimental Medicine and Hematology-Oncology, Brigham &amp; Women's Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115."
        }
      ]
    },
    {
      "given": "H. Randolph",
      "family": "Byers",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Pathology, Massachusetts General Hospital, Boston, MA 02114."
        }
      ]
    },
    {
      "given": "Thomas P.",
      "family": "Stossel",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Divisions of Experimental Medicine and Hematology-Oncology, Brigham &amp; Women's Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115."
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_2_1_1_1",
      "doi-asserted-by": "crossref",
      "first-page": "1477",
      "DOI": "10.1083/jcb.111.4.1477",
      "volume": "111",
      "year": "1990",
      "unstructured": "BRINK, M, A DICTYOSTELIUM MUTANT LACKING AN F-ACTIN CROSS-LINKING PROTEIN, THE 120-KD GELATION FACTOR, JOURNAL OF CELL BIOLOGY 111: 1477 (1990).",
      "journal-title": "JOURNAL OF CELL BIOLOGY"
    },
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "crossref",
      "first-page": "8988",
      "DOI": "10.1016/S0021-9258(17)34275-8",
      "volume": "253",
      "year": "1978",
      "unstructured": "BROTSCHI, E.A., GELATION OF ACTIN BY ACTIN-BINDING PROTEIN, JOURNAL OF BIOLOGICAL CHEMISTRY 253: 8988 (1978).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_3_1",
      "first-page": "423",
      "volume": "139",
      "year": "1991",
      "unstructured": "BYERS, H.R., CELL-MIGRATION AND ACTIN ORGANIZATION IN CULTURED HUMAN PRIMARY, RECURRENT CUTANEOUS AND METASTATIC MELANOMA - TIME-LAPSE AND IMAGE-ANALYSIS, AMERICAN JOURNAL OF PATHOLOGY 139: 423 (1991).",
      "journal-title": "AMERICAN JOURNAL OF PATHOLOGY"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00591a005"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "crossref",
      "first-page": "941",
      "DOI": "10.1083/jcb.75.3.941",
      "volume": "75",
      "year": "1977",
      "unstructured": "DAVIES, W.A., PERIPHERAL HYALINE BLEBS (PODOSOMES) OF MACROPHAGES, JOURNAL OF CELL BIOLOGY 75: 941 (1977).",
      "journal-title": "JOURNAL OF CELL BIOLOGY"
    },
    {
      "key": "e_1_2_1_6_1",
      "first-page": "169",
      "year": "1990",
      "unstructured": "Eicke, H.-F., Physical Networks: Polymers and Gels: 169 (1990).",
      "journal-title": "Physical Networks: Polymers and Gels"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "crossref",
      "first-page": "13303",
      "DOI": "10.1016/S0021-9258(18)37705-6",
      "volume": "263",
      "year": "1988",
      "unstructured": "EZZELL, R.M., LOCALIZATION OF THE DOMAIN OF ACTIN-BINDING PROTEIN THAT BINDS TO MEMBRANE GLYCOPROTEIN-IB AND ACTIN IN HUMAN-PLATELETS, JOURNAL OF BIOLOGICAL CHEMISTRY 263: 13303 (1988).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-2697(83)90418-9"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "crossref",
      "first-page": "11970",
      "DOI": "10.1016/S0021-9258(17)38972-X",
      "volume": "260",
      "year": "1985",
      "unstructured": "FOX, JEB, IDENTIFICATION OF ACTIN-BINDING PROTEIN AS THE PROTEIN LINKING THE MEMBRANE SKELETON TO GLYCOPROTEINS ON PLATELET PLASMA-MEMBRANES, JOURNAL OF BIOLOGICAL CHEMISTRY 260: 11970 (1985).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "crossref",
      "first-page": "1089",
      "DOI": "10.1083/jcb.111.3.1089",
      "volume": "111",
      "year": "1990",
      "unstructured": "GORLIN, J.B., HUMAN ENDOTHELIAL ACTIN-BINDING PROTEIN (ABP-280, NONMUSCLE FILAMIN) - A MOLECULAR LEAF SPRING, JOURNAL OF CELL BIOLOGY 111: 1089 (1990).",
      "journal-title": "JOURNAL OF CELL BIOLOGY"
    },
    {
      "key": "e_1_2_1_11_1",
      "first-page": "4831",
      "volume": "84",
      "year": "1987",
      "unstructured": "GUNNING, P, A HUMAN BETA-ACTIN EXPRESSION VECTOR SYSTEM DIRECTS HIGH-LEVEL ACCUMULATION OF ANTISENSE TRANSCRIPTS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 84: 4831 (1987).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "crossref",
      "first-page": "5696",
      "DOI": "10.1016/S0021-9258(19)41235-0",
      "volume": "250",
      "year": "1975",
      "unstructured": "HARTWIG, J.H., ISOLATION AND PROPERTIES OF ACTIN, MYOSIN, AND A NEW ACTIN-BINDING PROTEIN IN RABBIT ALVEOLAR MACROPHAGES, JOURNAL OF BIOLOGICAL CHEMISTRY 250: 5696 (1975).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "crossref",
      "first-page": "841",
      "DOI": "10.1083/jcb.87.3.841",
      "volume": "87",
      "year": "1980",
      "unstructured": "HARTWIG, J.H., ACTIN-BINDING PROTEIN PROMOTES THE BIPOLAR AND PERPENDICULAR BRANCHING OF ACTIN-FILAMENTS, JOURNAL OF CELL BIOLOGY 87: 841 (1980).",
      "journal-title": "JOURNAL OF CELL BIOLOGY"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "crossref",
      "first-page": "1571",
      "DOI": "10.1083/jcb.109.4.1571",
      "volume": "109",
      "year": "1989",
      "unstructured": "HARTWIG, J.H., ASSOCIATION OF PROFILIN WITH FILAMENT-FREE REGIONS OF HUMAN-LEUKOCYTE AND PLATELET MEMBRANES AND REVERSIBLE MEMBRANE-BINDING DURING PLATELET ACTIVATION, JOURNAL OF CELL BIOLOGY 109: 1571 (1989).",
      "journal-title": "JOURNAL OF CELL BIOLOGY"
    },
    {
      "key": "e_1_2_1_15_1",
      "first-page": "141",
      "year": "1991",
      "unstructured": "Hartwig, J. H., The Lung: Scientific Foundation: 141 (1991).",
      "journal-title": "The Lung: Scientific Foundation"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "crossref",
      "first-page": "41",
      "DOI": "10.1016/0165-022X(91)90080-G",
      "volume": "22",
      "year": "1991",
      "unstructured": "JANMEY, P.A., A TORSION PENDULUM FOR MEASUREMENT OF THE VISCOELASTICITY OF BIOPOLYMERS AND ITS APPLICATION TO ACTIN NETWORKS, JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS 22: 41 (1991).",
      "journal-title": "JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "crossref",
      "first-page": "13857",
      "DOI": "10.1016/S0021-9258(18)68322-X",
      "volume": "263",
      "year": "1988",
      "unstructured": "KWIATKOWSKI, D.J., PREDOMINANT INDUCTION OF GELSOLIN AND ACTIN-BINDING PROTEIN DURING MYELOID DIFFERENTIATION, JOURNAL OF BIOLOGICAL CHEMISTRY 263: 13857 (1988).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "crossref",
      "first-page": "369",
      "DOI": "10.1146/annurev.bb.17.060188.002101",
      "volume": "17",
      "year": "1988",
      "unstructured": "LUBYPHELPS, K, THE SUBMICROSCOPIC PROPERTIES OF CYTOPLASM AS A DETERMINANT OF CELLULAR FUNCTION, ANNUAL REVIEW OF BIOPHYSICS AND BIOPHYSICAL CHEMISTRY 17: 369 (1988).",
      "journal-title": "ANNUAL REVIEW OF BIOPHYSICS AND BIOPHYSICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "crossref",
      "first-page": "431",
      "DOI": "10.1016/0014-4827(81)90184-1",
      "volume": "135",
      "year": "1981",
      "unstructured": "MESLAND, DAM, CYTOCHALASIN-B DISRUPTS THE ASSOCIATION OF FILAMENTOUS WEB AND PLASMA-MEMBRANE IN HEPATOCYTES, EXPERIMENTAL CELL RESEARCH 135: 431 (1981).",
      "journal-title": "EXPERIMENTAL CELL RESEARCH"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "crossref",
      "first-page": "147",
      "DOI": "10.1016/0167-4838(85)90022-6",
      "volume": "830",
      "year": "1985",
      "unstructured": "MORROW, J, BIOCHIM BIOPHYS ACTA 830: 147 (1985).",
      "journal-title": "BIOCHIM BIOPHYS ACTA"
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "crossref",
      "first-page": "156",
      "DOI": "10.1016/0014-5793(87)80440-4",
      "volume": "224",
      "year": "1987",
      "unstructured": "NARVANEN, O, A MONOCLONAL-ANTIBODY AGAINST A SYNTHETIC PEPTIDE REVEALS COMMON STRUCTURES AMONG SPECTRINS AND ALPHA-ACTININ, FEBS LETTERS 224: 156 (1987).",
      "journal-title": "FEBS LETTERS"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "crossref",
      "first-page": "275",
      "DOI": "10.1016/0092-8674(91)90179-3",
      "volume": "67",
      "year": "1991",
      "unstructured": "OHTA, Y, LIGAND-SENSITIVE BINDING OF ACTIN-BINDING PROTEIN TO IMMUNOGLOBULIN-G FC RECEPTOR-I (FC-GAMMA-RI), CELL 67: 275 (1991).",
      "journal-title": "CELL"
    },
    {
      "key": "e_1_2_1_23_1",
      "doi-asserted-by": "crossref",
      "first-page": "317",
      "DOI": "10.1083/jcb.100.1.317",
      "volume": "100",
      "year": "1985",
      "unstructured": "OKITA, J.R., ON THE ASSOCIATION OF GLYCOPROTEIN 1B AND ACTIN-BINDING PROTEIN IN HUMAN-PLATELETS, JOURNAL OF CELL BIOLOGY 100: 317 (1985).",
      "journal-title": "JOURNAL OF CELL BIOLOGY"
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "crossref",
      "first-page": "987",
      "DOI": "10.1146/annurev.bi.55.070186.005011",
      "volume": "55",
      "year": "1986",
      "unstructured": "POLLARD, T.D., ACTIN AND ACTIN-BINDING PROTEINS - A CRITICAL-EVALUATION OF MECHANISMS AND FUNCTIONS, ANNUAL REVIEW OF BIOCHEMISTRY 55: 987 (1986).",
      "journal-title": "ANNUAL REVIEW OF BIOCHEMISTRY"
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "crossref",
      "first-page": "59",
      "DOI": "10.1242/jcs.90.1.59",
      "volume": "90",
      "year": "1988",
      "unstructured": "SCHLEICHER, M, A DICTYOSTELIUM MUTANT WITH SEVERE DEFECTS IN ALPHA-ACTININ - ITS CHARACTERIZATION USING CDNA PROBES AND MONOCLONAL-ANTIBODIES, JOURNAL OF CELL SCIENCE 90: 59 (1988).",
      "journal-title": "JOURNAL OF CELL SCIENCE"
    },
    {
      "key": "e_1_2_1_26_1",
      "first-page": "131",
      "year": "1987",
      "unstructured": "Stossel, T. P., Cytomechanics: 131 (1987).",
      "journal-title": "Cytomechanics"
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "crossref",
      "first-page": "18261",
      "DOI": "10.1016/S0021-9258(18)51454-X",
      "volume": "264",
      "year": "1989",
      "unstructured": "STOSSEL, T.P., FROM SIGNAL TO PSEUDOPOD - HOW CELLS CONTROL CYTOPLASMIC ACTIN ASSEMBLY, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 18261 (1989).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_28_1",
      "doi-asserted-by": "crossref",
      "first-page": "S15",
      "DOI": "10.1083/jcb.99.1.15s",
      "volume": "99",
      "year": "1984",
      "unstructured": "STOSSEL, T. P., JOURNAL OF CELL BIOLOGY 99: S15 (1984).",
      "journal-title": "JOURNAL OF CELL BIOLOGY"
    },
    {
      "key": "e_1_2_1_29_1",
      "first-page": "4483",
      "volume": "72",
      "year": "1975",
      "unstructured": "WANG, K, FILAMIN, A NEW HIGH-MOLECULAR-WEIGHT PROTEIN FOUND IN SMOOTH-MUSCLE AND NON-MUSCLE CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 72: 4483 (1975).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_30_1",
      "doi-asserted-by": "crossref",
      "first-page": "345",
      "DOI": "10.1016/S0005-8165(73)80085-X",
      "volume": "150",
      "year": "1973",
      "unstructured": "WEISS, E, ELECTRON-MICROSCOPY OF ISOLATED RAT HEPATOCYTES BEFORE AND AFTER TREATMENT WITH PHALLOIDIN, BEITRAGE ZUR PATHOLOGIE 150: 345 (1973).",
      "journal-title": "BEITRAGE ZUR PATHOLOGIE"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.1549777",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        11
      ]
    ],
    "date-time": "2024-01-11T09:25:11Z",
    "timestamp": 1704965111000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.1549777"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1992,
        1,
        17
      ]
    ]
  },
  "references-count": 30,
  "journal-issue": {
    "issue": "5042",
    "published-print": {
      "date-parts": [
        [
          1992,
          1,
          17
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.1549777"
  ],
  "URL": "http://dx.doi.org/10.1126/science.1549777",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1992,
        1,
        17
      ]
    ]
  }
}