DOI: 10.1126/science.1536005. The Elongation-Termination Decision in Transcription

The Elongation-Termination Decision in Transcription

10.1126/science.1536005

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

At any template position, the decision to extend the transcript by one residue or to release the nascent RNA represents a kinetic competition between elongation and termination pathways. This competition is discussed in terms of alternative Eyring transition state barriers; changes in termination efficiency correspond to small changes in the relative heights of these barriers. Elongation complexes are stable at nonterminator positions; a model is presented to explain the destabilization of these complexes at intrinsic termination sites. Functionally analogous effects can operate at rho-dependent terminators. Mechanisms for modulation of termination efficiency by regulatory proteins are described.

Bibliography

von Hippel, P. H., & Yager, T. D. (1992). The Elongation-Termination Decision in Transcription. Science, 255(5046), 809â812.

Authors 2

Peter H. von Hippel (first)
Thomas D. Yager (additional)

References 15 Referenced 66

ANDREWS, C, TRANSCRIPTION TERMINATION FACTOR-RHO MEDIATES SIMULTANEOUS RELEASE OF RNA TRANSCRIPTS AND DNA-TEMPLATE FROM COMPLEXES WITH ESCHERICHIA-COLI RNA-POLYMERASE, JOURNAL OF BIOLOGICAL CHEMISTRY 260: 5826 (1985). (10.1016/S0021-9258(18)89096-2) / JOURNAL OF BIOLOGICAL CHEMISTRY (1985)
ARNDT, K.M., RNA CHAIN ELONGATION BY ESCHERICHIA-COLI RNA-POLYMERASE - FACTORS AFFECTING THE STABILITY OF ELONGATING TERNARY COMPLEXES, JOURNAL OF MOLECULAR BIOLOGY 213: 79 (1990). (10.1016/S0022-2836(05)80123-8) / JOURNAL OF MOLECULAR BIOLOGY (1990)
BRENNAN, C.A., TRANSCRIPTION TERMINATION FACTOR-RHO IS AN RNA-DNA HELICASE, CELL 48: 945 (1987). (10.1016/0092-8674(87)90703-3) / CELL (1987)
GILL, S.C., THERMODYNAMIC ANALYSIS OF THE TRANSCRIPTION CYCLE IN ESCHERICHIA-COLI, BIOPHYSICAL CHEMISTRY 37: 239 (1990). (10.1016/0301-4622(90)88023-L) / BIOPHYSICAL CHEMISTRY (1990)
Jin, D. J., Proceedings of the National Academy of Sciences of the United States of America 89: 1453 (1992). / Proceedings of the National Academy of Sciences of the United States of America (1992)
MARTIN, F.H., DNA-RNA HYBRID DUPLEXES CONTAINING OLIGO(DA-RU) SEQUENCES ARE EXCEPTIONALLY UNSTABLE AND MAY FACILITATE TERMINATION OF TRANSCRIPTION, NUCLEIC ACIDS RESEARCH 8: 2295 (1980). (10.1093/nar/8.10.2295) / NUCLEIC ACIDS RESEARCH (1980)
MORGAN, W.D., RHO-DEPENDENT TERMINATION OF TRANSCRIPTION .2. KINETICS OF MESSENGER-RNA ELONGATION DURING TRANSCRIPTION FROM THE BACTERIOPHAGE-LAMBDA-PR PROMOTER, JOURNAL OF BIOLOGICAL CHEMISTRY 258: 9565 (1983). (10.1016/S0021-9258(17)44704-1) / JOURNAL OF BIOLOGICAL CHEMISTRY (1983)
RHODES, G, RIBONUCLEIC-ACID CHAIN ELONGATION BY ESCHERICHIA-COLI RIBONUCLEIC-ACID POLYMERASE .1. ISOLATION OF TERNARY COMPLEXES AND KINETICS OF ELONGATION, JOURNAL OF BIOLOGICAL CHEMISTRY 249: 6675 (1974). (10.1016/S0021-9258(19)42207-2) / JOURNAL OF BIOLOGICAL CHEMISTRY (1974)
RICE, G.A., FOOTPRINTING ANALYSIS OF MAMMALIAN RNA POLYMERASE-II ALONG ITS TRANSCRIPT - AN ALTERNATIVE VIEW OF TRANSCRIPTION ELONGATION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 4245 (1991). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1991)
VONHIPPEL, P.H., TRANSCRIPT ELONGATION AND TERMINATION ARE COMPETITIVE KINETIC PROCESSES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 2307 (1991). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1991)
WHELAN W.A. unpublished data.
YAGER, T.D., A THERMODYNAMIC ANALYSIS OF RNA TRANSCRIPT ELONGATION AND TERMINATION IN ESCHERICHIA-COLI, BIOCHEMISTRY 30: 1097 (1991). (10.1021/bi00218a032) / BIOCHEMISTRY (1991)
Yager, T. D., Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology: 1241 (1987). / Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology (1987)
YAGER T.D. unpublished data.
YANG, X.J., GENE-Q ANTITERMINATOR PROTEINS OF ESCHERICHIA-COLI PHAGE-82 AND PHAGE-LAMBDA SUPPRESS PAUSING BY RNA-POLYMERASE AT A RHO-DEPENDENT TERMINATOR AND AT OTHER SITES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 5301 (1989). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1989)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 4:25 a.m.)
Indexed	1 month, 3 weeks ago (July 11, 2025, 6:42 a.m.)
Issued	33 years, 6 months ago (Feb. 14, 1992)
Published	33 years, 6 months ago (Feb. 14, 1992)
Published Print	33 years, 6 months ago (Feb. 14, 1992)

Funders 0

None

BibTeX

@article{von_Hippel_1992, title={The Elongation-Termination Decision in Transcription}, volume={255}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1536005}, DOI={10.1126/science.1536005}, number={5046}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={von Hippel, Peter H. and Yager, Thomas D.}, year={1992}, month=feb, pages={809–812} }

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