DOI: 10.1126/science.153.3731.64. In Vitro Synthesis of an Infectious Mutant RNA with a Normal RNA Replicase

In Vitro Synthesis of an Infectious Mutant RNA with a Normal RNA Replicase

10.1126/science.153.3731.64

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

When purified Qβ-RNA replicase is presented alternately with two genetically different Qβ-RNA molecules, the RNA synthesized is identical to the initiating template. The results establish that the RNA is the instructive agent in the replicative process and hence that it satisfies the operational definition of a self-duplicating entity. The data also eliminate alternative explanations which do not involve self-propagation of the input RNA. An opportunity is now provided for studying the genetics and evolution of a self-duplicating nucleic acid molecule under conditions permitting detailed control of environmental parameters and chemical components.

Bibliography

Pace, N. R., & Spiegelman, S. (1966). In Vitro Synthesis of an Infectious Mutant RNA with a Normal RNA Replicase. Science, 153(3731), 64â67.

Authors 2

N. R. Pace (first)
S. Spiegelman (additional)

References 13 Referenced 21

DAVERN, C.I., INHIBITION + MUTAGENESIS OF RNA BACTERIOPHAGE BY 5-FLUOROURACIL, AUSTRALIAN JOURNAL OF BIOLOGICAL SCIENCES 17: 726 (1964). (10.1071/BI9640726) / AUSTRALIAN JOURNAL OF BIOLOGICAL SCIENCES (1964)
FRASER, D, THE AMINO ACID COMPOSITION OF BACTERIOPHAGE-T3, JOURNAL OF BIOLOGICAL CHEMISTRY 205: 291 (1953). (10.1016/S0021-9258(19)77254-8) / JOURNAL OF BIOLOGICAL CHEMISTRY (1953)
GUTHRIE, G.D., OBSERVATIONS ON INFECTION OF BACTERIAL PROTOPLASTS WITH DEOXYRIBONUCLEIC ACID OF BACTERIOPHAGE PHIX174, BIOCHIMICA ET BIOPHYSICA ACTA 72: 290 (1963). (10.1016/0926-6550(63)90342-6) / BIOCHIMICA ET BIOPHYSICA ACTA (1963)
Haruna I. Proceedings of the National Academy of Sciences of the United States of America in press.
HARUNA, I, AN RNA REPLICASE INDUCED BY AND SELECTIVE FOR A VIRAL RNA ISOLATION AND PROPERTIES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 50: 905 (1963). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1963)
HARUNA, I, SPECIFIC TEMPLATE REQUIREMENTS OF RNA REPLICASES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 54: 579 (1965). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1965)
HARUNA, I, RECOGNITION OF SIZE AND SEQUENCE BY AN RNA REPLICASE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 54: 1189 (1965). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1965)
10.1126/science.150.3698.884
OVERBY, L.R., COMPARISON OF 2 SEROLOGICALLY DISTINCT RIBONUCLEIC ACID BACTERIOPHAGES .I. PROPERTIES OF VIRAL PARTICLES, JOURNAL OF BACTERIOLOGY 91: 442 (1966). (10.1128/jb.91.1.442-448.1966) / JOURNAL OF BACTERIOLOGY (1966)
Pace, N., Proceedings of the National Academy of Sciences of the United States of America 55: 1608 (1966). / Proceedings of the National Academy of Sciences of the United States of America (1966)
SPIEGELMAN, S, SYNTHESIS OF A SELF-PROPAGATING AND INFECTIOUS NUCLEIC ACID WITH A PURIFIED ENZYME, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 54: 919 (1965). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1965)
STRAUSS, J.A., J MOL BIOL 10: 422 (1963). / J MOL BIOL (1963)
WATANABE, I, NISHON RINSHO 22: 243 (1964). / NISHON RINSHO (1964)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:47 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 2:58 a.m.)
Indexed	1 month ago (July 26, 2025, 4:50 a.m.)
Issued	59 years, 1 month ago (July 1, 1966)
Published	59 years, 1 month ago (July 1, 1966)
Published Print	59 years, 1 month ago (July 1, 1966)

Funders 0

None

BibTeX

@article{Pace_1966, title={In Vitro Synthesis of an Infectious Mutant RNA with a Normal RNA Replicase}, volume={153}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.153.3731.64}, DOI={10.1126/science.153.3731.64}, number={3731}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Pace, N. R. and Spiegelman, S.}, year={1966}, month=jul, pages={64–67} }

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