DOI: 10.1126/science.150.3698.884. Autocatalytic Synthesis of a Viral RNA in vitro

Autocatalytic Synthesis of a Viral RNA in vitro

10.1126/science.150.3698.884

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Experiments with an RNA-dependent RNA polymerase ("replicase") purified from Escherichia coli infected with an RNA bacteriophage (Qβ) demonstrate that the enzyme generates a polynucleotide of the same molecular weight as viral RNA; the "replicase" cannot distinguish the polynucleotide from its own RNA genome. By starting reactions at input ratios below the saturation levels of template to enzyme, autocatalytic kinetics of RNA increase are observed. The data are consistent with the conclusion that self-propagation of complete viral genomes is occurring in this simple system.

Bibliography

Haruna, I., & Spiegelman, S. (1965). Autocatalytic Synthesis of a Viral RNA in vitro. Science, 150(3698), 884â886.

Authors 2

I. Haruna (first)
S. Spiegelman (additional)

References 9 Referenced 96

GESTELAND, R. F., FEDERATION PROCEEDINGS 24: 293 (1965). / FEDERATION PROCEEDINGS (1965)
HARUNA I IN PREPARATION.
HARUNA, I, AN RNA REPLICASE INDUCED BY AND SELECTIVE FOR A VIRAL RNA ISOLATION AND PROPERTIES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 50: 905 (1963). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1963)
HARUNA, I, SPECIFIC TEMPLATE REQUIREMENTS OF RNA REPLICASES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 54: 579 (1965). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1965)
Overby, L., Journal of Bacteriology 91: 442 (1966). (10.1128/jb.91.1.442-448.1966) / Journal of Bacteriology (1966)
SPAHR, P.F., PURIFICATION AND MECHANISM OF ACTION OF RIBONUCLEASE FROM ESCHERICHIA COLI RIBOSOMES, JOURNAL OF BIOLOGICAL CHEMISTRY 236: 823 (1961). (10.1016/S0021-9258(18)64315-7) / JOURNAL OF BIOLOGICAL CHEMISTRY (1961)
SPAHR, P.F., PURIFICATION + PROPERTIES OF RIBONUCLEASE 2 FROM ESCHERICHIA COLI, JOURNAL OF BIOLOGICAL CHEMISTRY 239: 3716 (1964). (10.1016/S0021-9258(18)91195-6) / JOURNAL OF BIOLOGICAL CHEMISTRY (1964)
SPIEGELMAN, S, SYNTHESIS OF A SELF-PROPAGATING AND INFECTIOUS NUCLEIC ACID WITH A PURIFIED ENZYME, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 54: 919 (1965). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1965)
WATANABE, I, NISHON RINSHO 22: 243 (1964). / NISHON RINSHO (1964)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:04 a.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 5:03 a.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 6:46 a.m.)
Issued	59 years, 9 months ago (Nov. 12, 1965)
Published	59 years, 9 months ago (Nov. 12, 1965)
Published Print	59 years, 9 months ago (Nov. 12, 1965)

Funders 0

None

BibTeX

@article{Haruna_1965, title={Autocatalytic Synthesis of a Viral RNA in vitro}, volume={150}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.150.3698.884}, DOI={10.1126/science.150.3698.884}, number={3698}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Haruna, I. and Spiegelman, S.}, year={1965}, month=nov, pages={884–886} }

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