DOI: 10.1126/science.150.3692.64. Erythrocyte Membrane: Chemical Modification

Erythrocyte Membrane: Chemical Modification

10.1126/science.150.3692.64

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Erythrocytes treated with 1-fluoro-2,4-dinitrobenzene become permeable to Na + and K + , but not to small water-soluble nonelectrolytes or hemoglobin, and eventually lyse in isotonic buffer. Erythrocytes treated with 1,5-difluoro-2,4-dinitrobenzene become permeable to Na + and K + but do not lyse in buffer or in water, even after extraction with lipid solvents. The difluoro compound crosslinks the membrane and increases its strength. Both reagents appear to remove the positive fixed charge responsible for the cation impermeability of the normal cell.

Bibliography

Berg, H. C., Diamond, J. M., & Marfey, P. S. (1965). Erythrocyte Membrane: Chemical Modification. Science, 150(3692), 64â67.

Authors 3

Howard C. Berg (first)
Jared M. Diamond (additional)
Peter S. Marfey (additional)

References 4 Referenced 64

MARFEY, P.S., REACTION OF BOVINE PANCREATIC RIBONUCLEASE A WITH 1,5-DIFLUORO-2,4-DINITROBENZENE, JOURNAL OF BIOLOGICAL CHEMISTRY 240: 3264 (1965). (10.1016/S0021-9258(18)97213-3) / JOURNAL OF BIOLOGICAL CHEMISTRY (1965)
MARFEY, P.S., REACTION OF BOVINE PANCREATIC RIBONUCLEASE A WITH 1,5-DIFLUORO-2,4-DINITROBENZENE, JOURNAL OF BIOLOGICAL CHEMISTRY 240: 3270 (1965). (10.1016/S0021-9258(18)97214-5) / JOURNAL OF BIOLOGICAL CHEMISTRY (1965)
Whittam R. Transport and Diffusion in Red Blood Cells (1964).
ZAHN, H, REAKTIONEN VON 1,5-DIFLUOR-2,4-DINITROBENZOL MIT INSULIN .2. VERSUCHE MIT INSULIN, MAKROMOLEKULARE CHEMIE 26: 153 (1958). (10.1002/macp.1958.020260113) / MAKROMOLEKULARE CHEMIE (1958)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:04 a.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 8:57 p.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 12:35 p.m.)
Issued	59 years, 10 months ago (Oct. 1, 1965)
Published	59 years, 10 months ago (Oct. 1, 1965)
Published Print	59 years, 10 months ago (Oct. 1, 1965)

Funders 0

None

BibTeX

@article{Berg_1965, title={Erythrocyte Membrane: Chemical Modification}, volume={150}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.150.3692.64}, DOI={10.1126/science.150.3692.64}, number={3692}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Berg, Howard C. and Diamond, Jared M. and Marfey, Peter S.}, year={1965}, month=oct, pages={64–67} }

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