DOI: 10.1126/science.1496380. Mechanistic Aspects of Signaling Through Ras in NIH 3T3 Cells

Mechanistic Aspects of Signaling Through Ras in NIH 3T3 Cells

10.1126/science.1496380

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Serum and growth factors can increase the proportion of Ras in the active guanosine triphosphate (GTP)-bound form. Growth factors might stimulate guanine nucleotide exchange or decrease the activity of the guanosine triphosphatase-activating proteins GAP and neurofibromin (NF1). In NIH 3T3 cells that overexpress the mutant Ras protein His 116 , which releases bound guanine nucleotide at a constitutively high rate and retains sensitivity to GAP and NF1, the proportion of GTP bound to the His 116 protein was not altered by serum or platelet-derived growth factor. However, these mitogens increased the proportion of Ras in the GTP-bound form in cells that overexpressed control Ras proteins with a normal intrinsic rate of guanine nucleotide release. The amount of GTP-bound His 116 or control Ras proteins was higher in cells at low density than in cells at high density, which have more GAP-like activity. The lower proportion of GTP-bound Ras in NIH 3T3 cells at high density may result from increased GAP-like activity. By contrast, serum and platelet-derived growth factors appear to stimulate guanine nucleotide exchange.

Bibliography

Zhang, K., Papageorge, A. G., & Lowy, D. R. (1992). Mechanistic Aspects of Signaling Through Ras in NIH 3T3 Cells. Science, 257(5070), 671â674.

Authors 3

Ke Zhang (first)
Alex G. Papageorge (additional)
Douglas R. Lowy (additional)

References 34 Referenced 61

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10.1038/348125a0
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FURTH, M.E., MONOCLONAL-ANTIBODIES TO THE P21 PRODUCTS OF THE TRANSFORMING GENE OF HARVEY MURINE SARCOMA-VIRUS AND OF THE CELLULAR RAS GENE FAMILY, JOURNAL OF VIROLOGY 43: 294 (1982). (10.1128/jvi.43.1.294-304.1982) / JOURNAL OF VIROLOGY (1982)
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JONES, S, THE CDC25 PROTEIN OF SACCHAROMYCES-CEREVISIAE PROMOTES EXCHANGE OF GUANINE-NUCLEOTIDES BOUND TO RAS, MOLECULAR AND CELLULAR BIOLOGY 11: 2641 (1991). / MOLECULAR AND CELLULAR BIOLOGY (1991)
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10.1126/science.3547648
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SATOH, T, ACCUMULATION OF P21RAS.GTP IN RESPONSE TO STIMULATION WITH EPIDERMAL GROWTH-FACTOR AND ONCOGENE PRODUCTS WITH TYROSINE KINASE-ACTIVITY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 87: 7926 (1990). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1990)
SIGAL, I.S., MUTANT RAS-ENCODED PROTEINS WITH ALTERED NUCLEOTIDE BINDING EXERT DOMINANT BIOLOGICAL EFFECTS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 952 (1986). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1986)
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10.1126/science.2821624
10.1126/science.2181667
10.1016/0092-8674(90)90149-9
10.1038/346754a0

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 11:08 p.m.)
Indexed	1 year, 7 months ago (Jan. 15, 2024, 7 a.m.)
Issued	33 years ago (July 31, 1992)
Published	33 years ago (July 31, 1992)
Published Print	33 years ago (July 31, 1992)

Funders 0

None

BibTeX

@article{Zhang_1992, title={Mechanistic Aspects of Signaling Through Ras in NIH 3T3 Cells}, volume={257}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1496380}, DOI={10.1126/science.1496380}, number={5070}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Zhang, Ke and Papageorge, Alex G. and Lowy, Douglas R.}, year={1992}, month=jul, pages={671–674} }

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