DOI: 10.1126/science.1496376. Directed Evolution of an RNA Enzyme

10.1126/science.1496376

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

An in vitro evolution procedure was used to obtain RNA enzymes with a particular catalytic function. A population of 10 13 variants of the Tetrahymena ribozyme, a group I ribozyme that catalyzes sequence-specific cleavage of RNA via a phosphoester transfer mechanism, was generated. This enzyme has a limited ability to cleave DNA under conditions of high temperature or high MgCl 2 concentration, or both. A selection constraint was imposed on the population of ribozyme variants such that only those individuals that carried out DNA cleavage under physiologic conditions were amplified to produce "progeny" ribozymes. Mutations were introduced during amplification to maintain heterogeneity in the population. This process was repeated for ten successive generations, resulting in enhanced (100 times) DNA cleavage activity.

Bibliography

Beaudry, A. A., & Joyce, G. F. (1992). Directed Evolution of an RNA Enzyme. Science, 257(5070), 635â641.

Authors 2

Amber A. Beaudry (first)
Gerald F. Joyce (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 11:07 p.m.)
Indexed	3 weeks, 6 days ago (Aug. 2, 2025, 1:15 a.m.)
Issued	33 years ago (July 31, 1992)
Published	33 years ago (July 31, 1992)
Published Print	33 years ago (July 31, 1992)

Funders 0

None

BibTeX

@article{Beaudry_1992, title={Directed Evolution of an RNA Enzyme}, volume={257}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1496376}, DOI={10.1126/science.1496376}, number={5070}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Beaudry, Amber A. and Joyce, Gerald F.}, year={1992}, month=jul, pages={635–641} }

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