DOI: 10.1126/science.1455234. Regulation of the Differentiation of Teratocarcinoma Cells into Primitive Endoderm by Gβ <sub>i2</sub>

Regulation of the Differentiation of Teratocarcinoma Cells into Primitive Endoderm by Gβ i2

10.1126/science.1455234

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The amount of the heterotrimeric G protein subunit Gα i2 decreases after the induction of F9 teratocarcinoma cells to become primitive endoderm in the presence of retinoic acid (RA). The reduction of the Gα i2 protein in F9 cells by antisense RNA expression was associated with (i) loss of receptor-mediated inhibition of adenylyl cyclase; (ii) decreased cell doubling time; (iii) induction of a primitive, endoderm-like phenotype in the absence of RA; and (iv) production of the differentiation marker tissue-type plasminogen activator. Expression of a constitutively active, mutant Gα i2 blocked RA-induced differentiation. These data suggest the involvement of Gα i2 in the control of stem cell differentiation and provide insight into the involvement of G proteins in growth regulation.

Bibliography

Watkins, D. C., Johnson, G. L., & Malbon, C. C. (1992). Regulation of the Differentiation of Teratocarcinoma Cells into Primitive Endoderm by GÎ² i2. Science, 258(5086), 1373â1375.

Authors 3

David C. Watkins (first)
Gary L. Johnson (additional)
Craig C. Malbon (additional)

References 27 Referenced 80

ANDRADEGORDON, P, INTERACTION OF HEPARIN WITH PLASMINOGEN ACTIVATORS AND PLASMINOGEN - EFFECTS ON THE ACTIVATION OF PLASMINOGEN, BIOCHEMISTRY 25: 4033 (1986). (10.1021/bi00362a007) / BIOCHEMISTRY (1986)
BIRNBAUMER, L, RECEPTOR-EFFECTOR COUPLING BY G-PROTEINS, BIOCHIMICA ET BIOPHYSICA ACTA 1031: 163 (1990). (10.1016/0304-4157(90)90007-Y) / BIOCHIMICA ET BIOPHYSICA ACTA (1990)
10.1038/348125a0
10.1038/349117a0
BROWN, A.M., IONIC CHANNELS AND THEIR REGULATION BY G-PROTEIN SUBUNITS, ANNUAL REVIEW OF PHYSIOLOGY 52: 197 (1990). (10.1146/annurev.ph.52.030190.001213) / ANNUAL REVIEW OF PHYSIOLOGY (1990)
BROWN, B.L., SIMPLE AND SENSITIVE SATURATION ASSAY METHOD FOR MEASUREMENT OF ADENOSINE 3'-5'-CYCLIC MONOPHOSPHATE, BIOCHEMICAL JOURNAL 121: 561 (1971). (10.1042/bj1210561) / BIOCHEMICAL JOURNAL (1971)
DARROW, A.L., MAINTENANCE AND USE OF F9 TERATOCARCINOMA CELLS, METHODS IN ENZYMOLOGY 190: 110 (1990). (10.1016/0076-6879(90)90015-S) / METHODS IN ENZYMOLOGY (1990)
GALVINPARTON P unpublished data.
GALVINPARTON, P.A., RETINOIC ACID MODULATION OF TRANSMEMBRANE SIGNALING - ANALYSIS IN F9-TERATOCARCINOMA CELLS, JOURNAL OF BIOLOGICAL CHEMISTRY 265: 17771 (1990). (10.1016/S0021-9258(18)38230-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1990)
GILMAN, A.G., REGULATION OF ADENYLYL CYCLASE BY G-PROTEINS, ADVANCES IN SECOND MESSENGER AND PHOSPHOPROTEIN RESEARCH 24: 51 (1990). / ADVANCES IN SECOND MESSENGER AND PHOSPHOPROTEIN RESEARCH (1990)
GRIEP, A.E., ANTISENSE MYC SEQUENCES INDUCE DIFFERENTIATION OF F9 CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 6806 (1988). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1988)
GUPTA, S.K., ANALYSIS OF THE FIBROBLAST TRANSFORMATION POTENTIAL OF GTPASE-DEFICIENT GIP2 ONCOGENES, MOLECULAR AND CELLULAR BIOLOGY 12: 190 (1992). / MOLECULAR AND CELLULAR BIOLOGY (1992)
KLEBE, R.J., QUANTITATIVE ASSAY FOR MORPHOGENESIS INDICATES THE ROLE OF EXTRACELLULAR-MATRIX COMPONENTS AND G-PROTEINS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 9588 (1991). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1991)
LAMORTE, V.J., INHIBITION OF DNA-SYNTHESIS IN LIVING CELLS BY MICROINJECTION OF GI2 ANTIBODIES, JOURNAL OF BIOLOGICAL CHEMISTRY 267: 691 (1992). (10.1016/S0021-9258(18)48337-8) / JOURNAL OF BIOLOGICAL CHEMISTRY (1992)
LOWNDES, J.M., GTPASE-DEFICIENT G-ALPHA-I2 ONCOGENE GIP2 INHIBITS ADENYLYLCYCLASE AND ATTENUATES RECEPTOR-STIMULATED PHOSPHOLIPASE-A2 ACTIVITY, JOURNAL OF BIOLOGICAL CHEMISTRY 266: 14193 (1991). (10.1016/S0021-9258(18)98666-7) / JOURNAL OF BIOLOGICAL CHEMISTRY (1991)
10.1126/science.2116665
MARKOWITZ, D, CONSTRUCTION AND USE OF A SAFE AND EFFICIENT AMPHOTROPIC PACKAGING CELL-LINE, VIROLOGY 167: 400 (1988). (10.1016/S0042-6822(88)90101-8) / VIROLOGY (1988)
MCKENZIE, F.R., DELTA-OPIOID-RECEPTOR-MEDIATED INHIBITION OF ADENYLATE-CYCLASE IS TRANSDUCED SPECIFICALLY BY THE GUANINE-NUCLEOTIDE-BINDING PROTEIN GI2, BIOCHEMICAL JOURNAL 267: 391 (1990). (10.1042/bj2670391) / BIOCHEMICAL JOURNAL (1990)
MILLER, A.D., IMPROVED RETROVIRAL VECTORS FOR GENE-TRANSFER AND EXPRESSION, BIOTECHNIQUES 7: 980 (1989). / BIOTECHNIQUES (1989)
POUYSSEGUR, J, TRANSMEMBRANE RECEPTORS AND INTRACELLULAR PATHWAYS THAT CONTROL CELL-PROLIFERATION, ANNUAL REVIEW OF PHYSIOLOGY 54: 195 (1992). (10.1146/annurev.ph.54.030192.001211) / ANNUAL REVIEW OF PHYSIOLOGY (1992)
Sambrook J. Molecular Cloning: A Laboratory Manual 2 (1989).
STRICKLAND, S, INDUCTION OF DIFFERENTIATION IN TERATOCARCINOMA STEM-CELLS BY RETINOIC ACID, CELL 15: 393 (1978). (10.1016/0092-8674(78)90008-9) / CELL (1978)
STRICKLAND, S, HORMONAL INDUCTION OF DIFFERENTIATION IN TERATOCARCINOMA STEM-CELLS - GENERATION OF PARIETAL ENDODERM BY RETINOIC ACID AND DIBUTYRYL CAMP, CELL 21: 347 (1980). (10.1016/0092-8674(80)90471-7) / CELL (1980)
VANDERKROL, A.R., MODULATION OF EUKARYOTIC GENE-EXPRESSION BY COMPLEMENTARY RNA OR DNA-SEQUENCES, BIOTECHNIQUES 6: 958 (1988). / BIOTECHNIQUES (1988)
WANG, H.Y., ANTISENSE OLIGODEOXYNUCLEOTIDES TO G(S) PROTEIN ALPHA-SUBUNIT SEQUENCE ACCELERATE DIFFERENTIATION OF FIBROBLASTS TO ADIPOCYTES, NATURE 358: 334 (1992). (10.1038/358334a0) / NATURE (1992)
WATKINS D.C. unpublished data.
WONG, Y.H., MUTANT ALPHA-SUBUNITS OF GI2 INHIBIT CYCLIC-AMP ACCUMULATION, NATURE 351: 63 (1991). (10.1038/351063a0) / NATURE (1991)

Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 7:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 9:58 p.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 4:37 p.m.)
Issued	32 years, 9 months ago (Nov. 20, 1992)
Published	32 years, 9 months ago (Nov. 20, 1992)
Published Print	32 years, 9 months ago (Nov. 20, 1992)

Funders 0

None

BibTeX

@article{Watkins_1992, title={Regulation of the Differentiation of Teratocarcinoma Cells into Primitive Endoderm by Gβ i2}, volume={258}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1455234}, DOI={10.1126/science.1455234}, number={5086}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Watkins, David C. and Johnson, Gary L. and Malbon, Craig C.}, year={1992}, month=nov, pages={1373–1375} }

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