DOI: 10.1126/science.1439809. Chemical Contrast in X-Ray Microscopy and Spatially Resolved XANES Spectroscopy of Organic Specimens

Chemical Contrast in X-Ray Microscopy and Spatially Resolved XANES Spectroscopy of Organic Specimens

10.1126/science.1439809

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The scanning transmission x-ray microscope at the National Synchrotron Light Source has been used to record x-ray absorption near-edge structure (XANES) spectra from 0.01-square-micrometer regions of organic specimens. The spectral features observed reflect the molecular structure of the dominant absorbing atoms and provide the contrast mechanism for high-resolution imaging with chemical sensitivity. This technique was used with x-ray energies near the carbon K absorption edge to identify and map separate phases in various polymer blends and to map the DNA distribution in chromosomes with a spatial resolution of 55 nanometers.

Bibliography

Ade, H., Zhang, X., Cameron, S., Costello, C., Kirz, J., & Williams, S. (1992). Chemical Contrast in X-Ray Microscopy and Spatially Resolved XANES Spectroscopy of Organic Specimens. Science, 258(5084), 972â975.

Authors 6

H. Ade (first)
X. Zhang (additional)
S. Cameron (additional)
C. Costello (additional)
J. Kirz (additional)
S. Williams (additional)

References 20 Referenced 306

Anderson E. X-ray Microscopy III (1992).
BORGGREVE, RJM, BRITTLE TOUGH TRANSITION IN NYLON RUBBER BLENDS - EFFECT OF RUBBER CONCENTRATION AND PARTICLE-SIZE, POLYMER 28: 1489 (1987). (10.1016/0032-3861(87)90348-X) / POLYMER (1987)
BRIBER, P. M., JOURNAL OF POLYMER SCIENCE PART B: POLYMER PHYSICS 26: 621 (1988). (10.1002/polb.1988.090260313) / JOURNAL OF POLYMER SCIENCE PART B: POLYMER PHYSICS (1988)
CAZAUX, J, THE INFLUENCE OF RADIATION-DAMAGE (MICROSCOPIC CAUSES) ON THE SENSITIVITY OF AUGER-ELECTRON SPECTROSCOPY AND X-RAY PHOTOELECTRON-SPECTROSCOPY, APPLIED SURFACE SCIENCE 20: 457 (1985). (10.1016/0378-5963(85)90167-9) / APPLIED SURFACE SCIENCE (1985)
CIMMINO, S, POLYMER ENGINEERING AND SCIENCE 24: 48 (1984). (10.1002/pen.760240106) / POLYMER ENGINEERING AND SCIENCE (1984)
HIRAYAMA, K, RAPID CONFIRMATION AND REVISION OF THE PRIMARY STRUCTURE OF BOVINE SERUM-ALBUMIN BY ESIMS AND FRIT-FAB LC MS, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 173: 639 (1990). (10.1016/S0006-291X(05)80083-X) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1990)
ISAACSON, M, INTERACTION OF 25 KEV ELECTRONS WITH NUCLEIC-ACID BASES, ADENINE, THYMINE, AND URACIL .2. INNER SHELL EXCITATION AND INELASTIC-SCATTERING CROSS-SECTIONS, JOURNAL OF CHEMICAL PHYSICS 56: 1813 (1972). (10.1063/1.1677457) / JOURNAL OF CHEMICAL PHYSICS (1972)
JACOBSEN, C, DIFFRACTION-LIMITED IMAGING IN A SCANNING-TRANSMISSION X-RAY MICROSCOPE, OPTICS COMMUNICATIONS 86: 351 (1991). (10.1016/0030-4018(91)90016-7) / OPTICS COMMUNICATIONS (1991)
Kirtley, S. M., Biochimica et Biophysica Acta 1132: 249 (1992). (10.1016/0167-4781(92)90157-U) / Biochimica et Biophysica Acta (1992)
LAIBINIS, P.E., X-RAY-DAMAGE TO CF3CO2-TERMINATED ORGANIC MONOLAYERS ON SI/AU - PRINCIPAL EFFECT OF ELECTRONS, SCIENCE 254: 981 (1991). (10.1126/science.254.5034.981) / SCIENCE (1991)
LEAPMAN, R. D., JOURNAL OF MICROSCOPY-OXFORD 165: 225 (1992). (10.1111/j.1365-2818.1992.tb01482.x) / JOURNAL OF MICROSCOPY-OXFORD (1992)
RARBACK, H, JOURNAL OF X-RAY SCIENCE AND TECHNOLOGY 2: 274 (1990). / JOURNAL OF X-RAY SCIENCE AND TECHNOLOGY (1990)
Reimer L. Transmission Electron Microscopy (1988). (10.1007/978-3-662-21579-1)
SOLEM, J.C., X-RAY BIOMICROHOLOGRAPHY, OPTICAL ENGINEERING 23: 193 (1984). / OPTICAL ENGINEERING (1984)
10.1126/science.218.4569.229
Stohr J. NEXAFS Spectroscopy and the Structure of Adsorbed Molecules (1992). (10.1007/978-3-662-02853-7)
Williams, S., Journal of Microscopy 170: 155 (1993). (10.1111/j.1365-2818.1993.tb03335.x) / Journal of Microscopy (1993)
WU, S, POLYMER 26: 1855 (1987). / POLYMER (1987)
WU, S. H., JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS 21: 699 (1983). (10.1002/pol.1983.180210503) / JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS (1983)
WU, S. H., POLYMER ENGINEERING AND SCIENCE 27: 335 (1987). (10.1002/pen.760270506) / POLYMER ENGINEERING AND SCIENCE (1987)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 10:08 p.m.)
Indexed	1 month ago (July 23, 2025, 8:35 a.m.)
Issued	32 years, 9 months ago (Nov. 6, 1992)
Published	32 years, 9 months ago (Nov. 6, 1992)
Published Print	32 years, 9 months ago (Nov. 6, 1992)

Funders 0

None

BibTeX

@article{Ade_1992, title={Chemical Contrast in X-Ray Microscopy and Spatially Resolved XANES Spectroscopy of Organic Specimens}, volume={258}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1439809}, DOI={10.1126/science.1439809}, number={5084}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Ade, H. and Zhang, X. and Cameron, S. and Costello, C. and Kirz, J. and Williams, S.}, year={1992}, month=nov, pages={972–975} }

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