DOI: 10.1126/science.1439793. A Surface Protease and the Invasive Character of Plague

A Surface Protease and the Invasive Character of Plague

10.1126/science.1439793

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A 9.5-kilobase plasmid of Yersinia pestis , the causative agent of plague, is required for high virulence when mice are inoculated with the bacterium by subcutaneous injection. Inactivation of the plasmid gene pla , which encodes a surface protease, increased the median lethal dose of the bacteria for mice by a millionfold. Moreover, cloned pla was sufficient to restore segregants lacking the entire pla - bearing plasmid to full virulence. Both pla + strains injected subcutaneously and pla - mutants injected intravenously reached high titers in liver and spleen of infected mice, whereas pla - mutants injected subcutaneously failed to do so even though they establish a sustained local infection at the injection site. More inflammatory cells accumulated in lesions caused by the pla - mutants than in lesions produced by the pla + parent. The Pla protease was shown to be a plasminogen activator with unusual kinetic properties. It can also cleave complement C3 at a specific site.

Bibliography

Sodeinde, O. A., Subrahmanyam, Y. V. B. K., Stark, K., Quan, T., Bao, Y., & Goguen, J. D. (1992). A Surface Protease and the Invasive Character of Plague. Science, 258(5084), 1004â1007.

Authors 6

Ola A. Sodeinde (first)
Y. V. B. K. Subrahmanyam (additional)
Kimberly Stark (additional)
Thomas Quan (additional)
Youdi Bao (additional)
Jon D. Goguen (additional)

References 16 Referenced 391

BEESLEY, E. D., JOURNAL OF BACTERIOLOGY 94: 19 (1967). (10.1128/jb.94.1.19-26.1967) / JOURNAL OF BACTERIOLOGY (1967)
Bercouvier, H., Bergeys Manual of Systematic Bacteriology 1: 498 (1984). / Bergeys Manual of Systematic Bacteriology (1984)
BRUBAKER, R.R., PASTEURELLA PESTIS - ROLE OF PESTICIN I AND IRON IN EXPERIMENTAL PLAGUE, SCIENCE 149: 422 (1965). (10.1126/science.149.3682.422) / SCIENCE (1965)
Butler, T., Plague and Other Yersinia Infections: 118 (1983). / Plague and Other Yersinia Infections (1983)
DUNN, D.L., FIBRIN IN PERITONITIS .3. THE MECHANISM OF BACTERIAL TRAPPING BY POLYMERIZING FIBRIN, SURGERY 92: 513 (1982). / SURGERY (1982)
FINEGOLD, M. J., AMERICAN JOURNAL OF PATHOLOGY 53: 99 (1968). / AMERICAN JOURNAL OF PATHOLOGY (1968)
HOYLAERTS, M, KINETICS OF THE ACTIVATION OF PLASMINOGEN BY HUMAN-TISSUE PLASMINOGEN-ACTIVATOR - ROLE OF FIBRIN, JOURNAL OF BIOLOGICAL CHEMISTRY 257: 2912 (1982). (10.1016/S0021-9258(19)81051-7) / JOURNAL OF BIOLOGICAL CHEMISTRY (1982)
LOTTENBERG, R, METHODS IN ENZYMOLOGY 80: 341 (1981). (10.1016/S0076-6879(81)80030-4) / METHODS IN ENZYMOLOGY (1981)
MIGNATTI, P, TUMOR INVASION THROUGH THE HUMAN AMNIOTIC MEMBRANE - REQUIREMENT FOR A PROTEINASE CASCADE, CELL 47: 487 (1986). (10.1016/0092-8674(86)90613-6) / CELL (1986)
PELTZ, S.W., POSITIVE REGULATION OF ACTIVATION OF PLASMINOGEN BY UROKINASE - DIFFERENCES IN KM FOR (GLUTAMIC-ACID) PLASMINOGEN AND LYSINE-PLASMINOGEN AND EFFECT OF CERTAIN ALPHA,OMEGA-AMINO ACIDS, BIOCHEMISTRY 21: 2798 (1982). (10.1021/bi00540a035) / BIOCHEMISTRY (1982)
REED, L. J., AMERICAN JOURNAL OF HYGIENE 27: 493 (1938). / AMERICAN JOURNAL OF HYGIENE (1938)
ROSQVIST, R, INCREASED VIRULENCE OF YERSINIA-PSEUDOTUBERCULOSIS BY 2 INDEPENDENT MUTATIONS, NATURE 334: 522 (1988). (10.1038/334522a0) / NATURE (1988)
SODEINDE, O.A., GENETIC-ANALYSIS OF THE 9.5-KILOBASE VIRULENCE PLASMID OF YERSINIA-PESTIS, INFECTION AND IMMUNITY 56: 2743 (1988). (10.1128/iai.56.10.2743-2748.1988) / INFECTION AND IMMUNITY (1988)
SODEINDE, O.A., NUCLEOTIDE-SEQUENCE OF THE PLASMINOGEN-ACTIVATOR GENE OF YERSINIA-PESTIS - RELATIONSHIP TO OMPT OF ESCHERICHIA-COLI AND GENE-E OF SALMONELLA-TYPHIMURIUM, INFECTION AND IMMUNITY 57: 1517 (1989). (10.1128/iai.57.5.1517-1523.1989) / INFECTION AND IMMUNITY (1989)
STOPPELLI, M.P., AUTOCRINE SATURATION OF PROUROKINASE RECEPTORS ON HUMAN A431 CELLS, CELL 45: 675 (1986). (10.1016/0092-8674(86)90782-8) / CELL (1986)
VERHEIJEN, J.H., A SIMPLE, SENSITIVE SPECTROPHOTOMETRIC ASSAY FOR EXTRINSIC (TISSUE-TYPE) PLASMINOGEN-ACTIVATOR APPLICABLE TO MEASUREMENTS IN PLASMA, THROMBOSIS AND HAEMOSTASIS 48: 266 (1982). (10.1055/s-0038-1657277) / THROMBOSIS AND HAEMOSTASIS (1982)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 10:08 p.m.)
Indexed	4 weeks, 2 days ago (July 24, 2025, 7:07 a.m.)
Issued	32 years, 9 months ago (Nov. 6, 1992)
Published	32 years, 9 months ago (Nov. 6, 1992)
Published Print	32 years, 9 months ago (Nov. 6, 1992)

Funders 0

None

BibTeX

@article{Sodeinde_1992, title={A Surface Protease and the Invasive Character of Plague}, volume={258}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1439793}, DOI={10.1126/science.1439793}, number={5084}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Sodeinde, Ola A. and Subrahmanyam, Y. V. B. K. and Stark, Kimberly and Quan, Thomas and Bao, Youdi and Goguen, Jon D.}, year={1992}, month=nov, pages={1004–1007} }

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