DOI: 10.1126/science.1439791. A GDP Dissociation Inhibitor That Serves as a GTPase Inhibitor for the Ras-Like Protein CDC42Hs

A GDP Dissociation Inhibitor That Serves as a GTPase Inhibitor for the Ras-Like Protein CDC42Hs

10.1126/science.1439791

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Members of the family of Ras-related guanosine triphosphate (GTP) binding proteins appear to take part in the regulation of a number of biological processes, including cell growth and differentiation. Three different classes of proteins that regulate the GTP binding and GTP hydrolytic activities of the Ras family members have been identified. These different regulatory proteins inhibit guanosine diphosphate (GDP) dissociation (designated as GDIs), stimulate GDP dissociation and GDP-GTP exchange (designated as GDSs), or stimulate GTP hydrolysis (designated as GAPs). In the case of the Ras-like protein CDC42Hs, which is the human homolog of a Saccharomyces cerevisiae cell division cycle protein, the GDI protein also inhibited both the intrinsic and GAP-stimulated hydrolysis of GTP. These findings establish an additional role for the GDI protein—namely, as a guanosine triphosphatase (GTPase) inhibitory protein for a Ras-like GTP binding protein.

Bibliography

Hart, M. J., Maru, Y., Leonard, D., Witte, O. N., Evans, T., & Cerione, R. A. (1992). A GDP Dissociation Inhibitor That Serves as a GTPase Inhibitor for the Ras-Like Protein CDC42Hs. Science, 258(5083), 812â815.

Authors 6

Matthew J. Hart (first)
Yoshiro Maru (additional)
David Leonard (additional)
Owen N. Witte (additional)
Tony Evans (additional)
Richard A. Cerione (additional)

References 28 Referenced 125

10.1038/353668a0
ADAMS, J.M., THE HEMATOPOIETICALLY EXPRESSED VAV PROTOONCOGENE SHARES HOMOLOGY WITH THE DBL GDP-GTP EXCHANGE FACTOR, THE BCR GENE AND A YEAST GENE (CDC24) INVOLVED IN CYTOSKELETAL ORGANIZATION, ONCOGENE 7: 611 (1992). / ONCOGENE (1992)
ARAKI, S, REGULATION OF REVERSIBLE BINDING OF SMG P25A, A RAS P21-LIKE GTP-BINDING PROTEIN, TO SYNAPTIC PLASMA-MEMBRANES AND VESICLES BY ITS SPECIFIC REGULATORY PROTEIN, GDP DISSOCIATION INHIBITOR, JOURNAL OF BIOLOGICAL CHEMISTRY 265: 13007 (1990). (10.1016/S0021-9258(19)38260-2) / JOURNAL OF BIOLOGICAL CHEMISTRY (1990)
ARBO, A, J BIOL CHEM 266: 23577 (1991). (10.1016/S0021-9258(18)54322-2) / J BIOL CHEM (1991)
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Cerione, R. A., Receptor-Effector Coupling: A Practical Approach: 69 (1990). / Receptor-Effector Coupling: A Practical Approach (1990)
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DOWNWARD, J, STIMULATION OF P21RAS UPON T-CELL ACTIVATION, NATURE 346: 719 (1990). (10.1038/346719a0) / NATURE (1990)
EVA, A, ISOLATION OF A NEW HUMAN ONCOGENE FROM A DIFFUSE B-CELL LYMPHOMA, NATURE 316: 273 (1985). (10.1038/316273a0) / NATURE (1985)
FUKUMOTO, Y, MOLECULAR-CLONING AND CHARACTERIZATION OF A NOVEL TYPE OF REGULATORY PROTEIN (GDI) FOR THE RHO PROTEINS, RAS P21-LIKE SMALL GTP-BINDING PROTEINS, ONCOGENE 5: 1321 (1990). / ONCOGENE (1990)
GARRETT, M.D., IDENTIFICATION OF DISTINCT CYTOPLASMIC TARGETS FOR RAS R-RAS AND RHO-REGULATORY PROTEINS, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 10 (1989). (10.1016/S0021-9258(17)31215-2) / JOURNAL OF BIOLOGICAL CHEMISTRY (1989)
HALL, A, SIGNAL TRANSDUCTION THROUGH SMALL GTPASES - A TALE OF 2 GAPS, CELL 69: 389 (1992). (10.1016/0092-8674(92)90441-E) / CELL (1992)
HALL, C, NOVEL HUMAN-BRAIN CDNA-ENCODING A 34,000 MR PROTEIN N-CHIMAERIN, RELATED TO BOTH THE REGULATORY DOMAIN OF PROTEIN KINASE-C AND BCR, THE PRODUCT OF THE BREAKPOINT CLUSTER REGION GENE, JOURNAL OF MOLECULAR BIOLOGY 211: 11 (1990). (10.1016/0022-2836(90)90006-8) / JOURNAL OF MOLECULAR BIOLOGY (1990)
HART, M.J., IDENTIFICATION OF THE HUMAN PLATELET GTPASE ACTIVATING PROTEIN FOR THE CDC42HS PROTEIN, JOURNAL OF BIOLOGICAL CHEMISTRY 266: 20840 (1991). (10.1016/S0021-9258(18)54786-4) / JOURNAL OF BIOLOGICAL CHEMISTRY (1991)
HART, M.J., CATALYSIS OF GUANINE-NUCLEOTIDE EXCHANGE ON THE CDC42HS PROTEIN BY THE DBL ONCOGENE PRODUCT, NATURE 354: 311 (1991). (10.1038/354311a0) / NATURE (1991)
HART M.J. unpublished data.
HEISTERKAMP, N, STRUCTURAL ORGANIZATION OF THE BCR GENE AND ITS ROLE IN THE PH' TRANSLOCATION, NATURE 315: 758 (1985). (10.1038/315758a0) / NATURE (1985)
HIRAOKA, K, BOTH STIMULATORY AND INHIBITORY GDP/GTP EXCHANGE PROTEINS, SMG GDS AND RHO GDI, ARE ACTIVE ON MULTIPLE SMALL GTP-BINDING PROTEINS, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 182: 921 (1992). (10.1016/0006-291X(92)91820-G) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1992)
KATZAV, S, VAV, A NOVEL HUMAN ONCOGENE DERIVED FROM A LOCUS UBIQUITOUSLY EXPRESSED IN HEMATOPOIETIC-CELLS, EMBO JOURNAL 8: 2283 (1989). (10.1002/j.1460-2075.1989.tb08354.x) / EMBO JOURNAL (1989)
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SETTLEMAN, J, MOLECULAR-CLONING OF CDNAS ENCODING THE GAP-ASSOCIATED PROTEIN P190 - IMPLICATIONS FOR A SIGNALING PATHWAY FROM RAS TO THE NUCLEUS, CELL 69: 539 (1992). (10.1016/0092-8674(92)90454-K) / CELL (1992)
10.1126/science.2237442
UEDA, T, PURIFICATION AND CHARACTERIZATION FROM BOVINE BRAIN CYTOSOL OF A NOVEL REGULATORY PROTEIN INHIBITING THE DISSOCIATION OF GDP FROM AND THE SUBSEQUENT BINDING OF GTP TO RHOB P20, A RAS P21-LIKE GTP-BINDING PROTEIN, JOURNAL OF BIOLOGICAL CHEMISTRY 265: 9373 (1990). (10.1016/S0021-9258(19)38859-3) / JOURNAL OF BIOLOGICAL CHEMISTRY (1990)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 9:54 p.m.)
Indexed	2 months, 1 week ago (June 25, 2025, 7:49 p.m.)
Issued	32 years, 10 months ago (Oct. 30, 1992)
Published	32 years, 10 months ago (Oct. 30, 1992)
Published Print	32 years, 10 months ago (Oct. 30, 1992)

Funders 0

None

BibTeX

@article{Hart_1992, title={A GDP Dissociation Inhibitor That Serves as a GTPase Inhibitor for the Ras-Like Protein CDC42Hs}, volume={258}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1439791}, DOI={10.1126/science.1439791}, number={5083}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Hart, Matthew J. and Maru, Yoshiro and Leonard, David and Witte, Owen N. and Evans, Tony and Cerione, Richard A.}, year={1992}, month=oct, pages={812–815} }

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