DOI: 10.1126/science.1280858. Solution Structure of the SH3 Domain of Src and Identification of Its Ligand-Binding Site

Solution Structure of the SH3 Domain of Src and Identification of Its Ligand-Binding Site

10.1126/science.1280858

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The Src homology 3 (SH3) region is a protein domain of 55 to 75 amino acids found in many cytoplasmic proteins, including those that participate in signal transduction pathways. The solution structure of the SH3 domain of the tyrosine kinase Src was determined by multidimensional nuclear magnetic resonance methods. The molecule is composed of two short three-stranded anti-parallel β sheets packed together at approximately right angles. Studies of the SH3 domain bound to proline-rich peptide ligands revealed a hydrophobic binding site on the surface of the protein that is lined with the side chains of conserved aromatic amino acids.

Bibliography

Yu, H., Rosen, M. K., Shin, T. B., Seidel-Dugan, C., Brugge, J. S., & Schreiber, S. L. (1992). Solution Structure of the SH3 Domain of Src and Identification of Its Ligand-Binding Site. Science, 258(5088), 1665â1668.

Authors 6

Hongtao Yu (first)
Michael K. Rosen (additional)
Tae Bum Shin (additional)
Cynthia Seidel-Dugan (additional)
Joan S. Brugge (additional)
Stuart L. Schreiber (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 4:05 p.m.)
Indexed	4 weeks ago (July 30, 2025, 11:03 a.m.)
Issued	32 years, 8 months ago (Dec. 4, 1992)
Published	32 years, 8 months ago (Dec. 4, 1992)
Published Print	32 years, 8 months ago (Dec. 4, 1992)

Funders 0

None

BibTeX

@article{Yu_1992, title={Solution Structure of the SH3 Domain of Src and Identification of Its Ligand-Binding Site}, volume={258}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1280858}, DOI={10.1126/science.1280858}, number={5088}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Yu, Hongtao and Rosen, Michael K. and Shin, Tae Bum and Seidel-Dugan, Cynthia and Brugge, Joan S. and Schreiber, Stuart L.}, year={1992}, month=dec, pages={1665–1668} }

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