DOI: 10.1126/science.1235490. Elongation Factor G Bound to the Ribosome in an Intermediate State of Translocation

Elongation Factor G Bound to the Ribosome in an Intermediate State of Translocation

10.1126/science.1235490

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Revealed in Translation The ribosome, with the help of transfer RNAs (tRNAs), converts the triple genetic code in messenger RNA (mRNA) into protein. Upon decoding of a codon, the mRNA and associated tRNAs must be moved through the ribosome, so that the next codon can be read, with a new charged tRNA taken in at the A (aminoacyl-tRNA) site, the newly extended peptidyl-tRNA moved into the P (peptidyl-tRNA) site, and the deacylated tRNA removed from the exit site in the ribosome (see the Perspective by Rodnina ). Crystal structures from Tourigny et al. (p. 1235490 ), Pulk and Cate (p. 1235970 ), and Zhou et al. (p. 1236086 ), variously capture the prokaryotic ribosome during this translocation phase, revealing the hybrid states of the tRNAs and the substantial motions of the 30S ribosomal subunit during the process, the role of elongation factor G, and suggest how the direction and reading frame of the mRNA is maintained.

Bibliography

Tourigny, D. S., FernÃ¡ndez, I. S., Kelley, A. C., & Ramakrishnan, V. (2013). Elongation Factor G Bound to the Ribosome in an Intermediate State of Translocation. Science, 340(6140).

Authors 4

David S. Tourigny (first)
Israel S. Fernández (additional)
Ann C. Kelley (additional)
V. Ramakrishnan (additional)

References 50 Referenced 193

Dates

Type	When
Created	12 years, 2 months ago (June 27, 2013, 2:06 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 9:37 a.m.)
Indexed	4 weeks, 2 days ago (July 30, 2025, 9:05 a.m.)
Issued	12 years, 2 months ago (June 28, 2013)
Published	12 years, 2 months ago (June 28, 2013)
Published Print	12 years, 2 months ago (June 28, 2013)

Funders 0

None

BibTeX

@article{Tourigny_2013, title={Elongation Factor G Bound to the Ribosome in an Intermediate State of Translocation}, volume={340}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1235490}, DOI={10.1126/science.1235490}, number={6140}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Tourigny, David S. and Fernández, Israel S. and Kelley, Ann C. and Ramakrishnan, V.}, year={2013}, month=jun }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        30
      ]
    ],
    "date-time": "2025-07-30T13:05:01Z",
    "timestamp": 1753880701035,
    "version": "3.41.2"
  },
  "reference-count": 50,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "6140",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2013,
        6,
        28
      ]
    ]
  },
  "abstract": "<jats:title>Revealed in Translation</jats:title>\n          <jats:p>\n            The ribosome, with the help of transfer RNAs (tRNAs), converts the triple genetic code in messenger RNA (mRNA) into protein. Upon decoding of a codon, the mRNA and associated tRNAs must be moved through the ribosome, so that the next codon can be read, with a new charged tRNA taken in at the A (aminoacyl-tRNA) site, the newly extended peptidyl-tRNA moved into the P (peptidyl-tRNA) site, and the deacylated tRNA removed from the exit site in the ribosome (see the Perspective by\n            <jats:bold>\n              <jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" ext-link-type=\"uri\" xlink:href=\"http://www.sciencemag.org/content/340/6140/1534.full\">Rodnina</jats:ext-link>\n            </jats:bold>\n            ). Crystal structures from\n            <jats:bold>\n              Tourigny\n              <jats:italic>et al.</jats:italic>\n            </jats:bold>\n            (p.\n            <jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" ext-link-type=\"uri\" xlink:href=\"http://www.sciencemag.org/content/340/6140/1235490.full\">1235490</jats:ext-link>\n            ),\n            <jats:bold>Pulk and Cate</jats:bold>\n            (p.\n            <jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" ext-link-type=\"uri\" xlink:href=\"http://www.sciencemag.org/content/340/6140/1235970.full\">1235970</jats:ext-link>\n            ), and\n            <jats:bold>\n              Zhou\n              <jats:italic>et al.</jats:italic>\n            </jats:bold>\n            (p.\n            <jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" ext-link-type=\"uri\" xlink:href=\"http://www.sciencemag.org/content/340/6140/1236086.full\">1236086</jats:ext-link>\n            ), variously capture the prokaryotic ribosome during this translocation phase, revealing the hybrid states of the tRNAs and the substantial motions of the 30S ribosomal subunit during the process, the role of elongation factor G, and suggest how the direction and reading frame of the mRNA is maintained.\n          </jats:p>",
  "DOI": "10.1126/science.1235490",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2013,
        6,
        27
      ]
    ],
    "date-time": "2013-06-27T18:06:26Z",
    "timestamp": 1372356386000
  },
  "source": "Crossref",
  "is-referenced-by-count": 193,
  "title": "Elongation Factor G Bound to the Ribosome in an Intermediate State of Translocation",
  "prefix": "10.1126",
  "volume": "340",
  "author": [
    {
      "given": "David S.",
      "family": "Tourigny",
      "sequence": "first",
      "affiliation": [
        {
          "name": "MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK."
        }
      ]
    },
    {
      "given": "Israel S.",
      "family": "Fern\u00e1ndez",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK."
        }
      ]
    },
    {
      "given": "Ann C.",
      "family": "Kelley",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK."
        }
      ]
    },
    {
      "given": "V.",
      "family": "Ramakrishnan",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK."
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_3_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/342142a0"
    },
    {
      "key": "e_1_3_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0809587105"
    },
    {
      "key": "e_1_3_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.molcel.2008.10.001"
    },
    {
      "key": "e_1_3_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35018597"
    },
    {
      "key": "e_1_3_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nsmb1060"
    },
    {
      "key": "e_1_3_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1261/rna.601507"
    },
    {
      "key": "e_1_3_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jmb.2007.04.042"
    },
    {
      "key": "e_1_3_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1994.tb06376.x"
    },
    {
      "key": "e_1_3_2_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1994.tb06675.x"
    },
    {
      "key": "e_1_3_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.270.5241.1464"
    },
    {
      "key": "e_1_3_2_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(03)00476-8"
    },
    {
      "key": "e_1_3_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.molcel.2007.01.027"
    },
    {
      "key": "e_1_3_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature09547"
    },
    {
      "key": "e_1_3_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)42519-2"
    },
    {
      "key": "e_1_3_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi970610k"
    },
    {
      "key": "e_1_3_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/385037a0"
    },
    {
      "key": "e_1_3_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(03)00230-2"
    },
    {
      "key": "e_1_3_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.molcel.2011.03.024"
    },
    {
      "key": "e_1_3_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-2836(03)00947-1"
    },
    {
      "key": "e_1_3_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1194460"
    },
    {
      "key": "e_1_3_2_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1202472"
    },
    {
      "key": "e_1_3_2_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1202472"
    },
    {
      "key": "e_1_3_2_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.1105714108"
    },
    {
      "key": "e_1_3_2_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1261/rna.031187.111"
    },
    {
      "key": "e_1_3_2_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1179709"
    },
    {
      "key": "e_1_3_2_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nsmb.2011"
    },
    {
      "key": "e_1_3_2_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1202692"
    },
    {
      "key": "e_1_3_2_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.1112185108"
    },
    {
      "key": "e_1_3_2_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.1201288109"
    },
    {
      "key": "e_1_3_2_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1117230"
    },
    {
      "key": "e_1_3_2_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1131127"
    },
    {
      "key": "e_1_3_2_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.1218999109"
    },
    {
      "key": "e_1_3_2_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.molcel.2008.03.012"
    },
    {
      "key": "e_1_3_2_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0813180106"
    },
    {
      "key": "e_1_3_2_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0908597107"
    },
    {
      "key": "e_1_3_2_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.febslet.2005.07.016"
    },
    {
      "key": "e_1_3_2_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/334362a0"
    },
    {
      "key": "e_1_3_2_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1175275"
    },
    {
      "key": "e_1_3_2_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35030019"
    },
    {
      "key": "e_1_3_2_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/cb700100n"
    },
    {
      "key": "e_1_3_2_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jmb.2012.03.016"
    },
    {
      "key": "e_1_3_2_43_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M002656200"
    },
    {
      "key": "e_1_3_2_44_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0909049509035377"
    },
    {
      "key": "e_1_3_2_45_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0907444909047337"
    },
    {
      "key": "e_1_3_2_46_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0907444909042589"
    },
    {
      "key": "e_1_3_2_47_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0907444909052925"
    },
    {
      "key": "e_1_3_2_48_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0907444911001314"
    },
    {
      "key": "e_1_3_2_49_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0907444910007493"
    },
    {
      "key": "e_1_3_2_50_2",
      "unstructured": "W. L. DeLano The PyMOL Molecular Graphics System; www.pymol.org (2006)."
    },
    {
      "key": "e_1_3_2_51_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/bioinformatics/btp033"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.1235490",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        10
      ]
    ],
    "date-time": "2024-01-10T14:37:20Z",
    "timestamp": 1704897440000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.1235490"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2013,
        6,
        28
      ]
    ]
  },
  "references-count": 50,
  "journal-issue": {
    "issue": "6140",
    "published-print": {
      "date-parts": [
        [
          2013,
          6,
          28
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.1235490"
  ],
  "URL": "http://dx.doi.org/10.1126/science.1235490",
  "relation": {
    "has-review": [
      {
        "id-type": "doi",
        "id": "10.3410/f.718025066.793484136",
        "asserted-by": "object"
      }
    ]
  },
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        2013,
        6,
        28
      ]
    ]
  },
  "article-number": "1235490"
}