DOI: 10.1126/science.122.3164.319. Acyl Derivatives of Glyceraldehyde-3-Phosphate Dehydrogenase

Acyl Derivatives of Glyceraldehyde-3-Phosphate Dehydrogenase

10.1126/science.122.3164.319

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Acetyl phosphate and 1,3-diphosphoglycerate react with glyceraldehyde-3-phosphate dehydrogenase to form relatively stable enzyme substrate compounds. These compounds appear to be thiol esters, and their properties indicate that they are intermediates in the catalytic activity of the enzyme: they undergo hydrolysis and arsenolysis in the presence of DPN and are reduced by DPNH to form aldehydes. These results are in agreement with the mechanism previously proposed for the oxidation of aldehydes in which a thiol ester formed on the enzyme, with concomitant reduction of DPN, is split in the presence of phosphate to acyl phosphate and regenerated enzyme.

Bibliography

Krimsky, I., & Racker, E. (1955). Acyl Derivatives of Glyceraldehyde-3-Phosphate Dehydrogenase. Science, 122(3164), 319â321.

Authors 2

I. Krimsky (first)
E. Racker (additional)

References 20 Referenced 93

BUCHER, T, BEZIEHUNG DES OXYDIERENDEN GARUNGSFERMENTS ZU FISCHER-ESTER BEI VERSCHIEDENEN ARSENIATKONZENTRATIONEN, BIOCHIMICA ET BIOPHYSICA ACTA 8: 220 (1952). (10.1016/0006-3002(52)90034-6) / BIOCHIMICA ET BIOPHYSICA ACTA (1952)
CORI, G.T., CRYSTALLINE D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM RABBIT MUSCLE, JOURNAL OF BIOLOGICAL CHEMISTRY 173: 605 (1948). (10.1016/S0021-9258(18)57432-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1948)
HARTING, J, FEDERATION PROCEEDINGS 12: 714 (1953). / FEDERATION PROCEEDINGS (1953)
Harting, J., Mechanism of Enzyme Action: 536 (1954). / Mechanism of Enzyme Action (1954)
KREBS, E.G., YEAST GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE .2. YEAST PROTEIN-2, JOURNAL OF BIOLOGICAL CHEMISTRY 200: 479 (1953). (10.1016/S0021-9258(18)71392-6) / JOURNAL OF BIOLOGICAL CHEMISTRY (1953)
KRIMSKY, I, FEDERATION PROCEEDINGS 13: 245 (1954). / FEDERATION PROCEEDINGS (1954)
KRIMSKY, I, FEDERATION PROCEEDINGS 14: 239 (1955). / FEDERATION PROCEEDINGS (1955)
KRIMSKY, I, GLUTATHIONE, A PROSTHETIC GROUP OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE, JOURNAL OF BIOLOGICAL CHEMISTRY 198: 721 (1952). (10.1016/S0021-9258(18)55529-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1952)
MEYERHOF, O, THE MECHANISM OF THE OXIDATIVE REACTION IN FERMENTATION, JOURNAL OF BIOLOGICAL CHEMISTRY 170: 1 (1947). (10.1016/S0021-9258(17)34929-3) / JOURNAL OF BIOLOGICAL CHEMISTRY (1947)
OESPER, P, THE MECHANISM OF ACTION OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE, JOURNAL OF BIOLOGICAL CHEMISTRY 207: 421 (1954). (10.1016/S0021-9258(18)71283-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1954)
RACKER, E, THE MECHANISM OF OXIDATION OF ALDEHYDES BY GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE, JOURNAL OF BIOLOGICAL CHEMISTRY 198: 731 (1952). (10.1016/S0021-9258(18)55530-7) / JOURNAL OF BIOLOGICAL CHEMISTRY (1952)
RACKER, E, THE MECHANISM OF ACTION OF GLYOXALASE, JOURNAL OF BIOLOGICAL CHEMISTRY 190: 685 (1951). (10.1016/S0021-9258(18)56017-8) / JOURNAL OF BIOLOGICAL CHEMISTRY (1951)
RACKER, E, MECHANISM OF ACTION AND PROPERTIES OF PYRIDINE NUCLEOTIDE-LINKED ENZYMES, PHYSIOLOGICAL REVIEWS 35: 1 (1955). (10.1152/physrev.1955.35.1.1) / PHYSIOLOGICAL REVIEWS (1955)
Racker E. A Symposium on Glutathione (1954).
SEGAL, H.L., THE ROLE OF SULFHYDRYL GROUPS IN THE ACTIVITY OF D-GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE, JOURNAL OF BIOLOGICAL CHEMISTRY 204: 265 (1953). (10.1016/S0021-9258(18)66136-8) / JOURNAL OF BIOLOGICAL CHEMISTRY (1953)
TAYLOR, J.F., THE PROSTHETIC GROUP OF CRYSTALLINE D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE, JOURNAL OF BIOLOGICAL CHEMISTRY 173: 619 (1948). (10.1016/S0021-9258(18)57433-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1948)
Taylor, J. F., A Symposium on Phosphorus Metabolism 1: 104 (1954). / A Symposium on Phosphorus Metabolism (1954)
VELICK, S.F., PHOSPHATE BINDING AND THE GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE REACTION, JOURNAL OF BIOLOGICAL CHEMISTRY 203: 545 (1953). (10.1016/S0021-9258(19)52325-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1953)
WARBURG, O, Isolation and crystallisation of the protein of oxidising fermenting enzymes, BIOCHEMISCHE ZEITSCHRIFT 303: 40 (1939). / BIOCHEMISCHE ZEITSCHRIFT (1939)
WARBURG, O, ZEITSCHRIFT FUR NATURFORSCHUNG SECTION B-A JOURNAL OF CHEMICAL SCIENCES 9: 391 (1954). (10.1515/znb-1954-0602) / ZEITSCHRIFT FUR NATURFORSCHUNG SECTION B-A JOURNAL OF CHEMICAL SCIENCES (1954)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 3:08 a.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 9:26 a.m.)
Indexed	1 year, 7 months ago (Jan. 10, 2024, 9:56 p.m.)
Issued	70 years ago (Aug. 19, 1955)
Published	70 years ago (Aug. 19, 1955)
Published Print	70 years ago (Aug. 19, 1955)

Funders 0

None

BibTeX

@article{Krimsky_1955, title={Acyl Derivatives of Glyceraldehyde-3-Phosphate Dehydrogenase}, volume={122}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.122.3164.319}, DOI={10.1126/science.122.3164.319}, number={3164}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Krimsky, I. and Racker, E.}, year={1955}, month=aug, pages={319–321} }

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