DOI: 10.1126/science.1191035. Substrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in Culture

Substrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in Culture

10.1126/science.1191035

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Environment Matters Stem cells isolated from muscle can be used for muscle regeneration, but only if the stem cells are fresh. Under standard cell culture conditions in the laboratory, muscle stem cells fail to proliferate efficiently and lose their regenerative capacity. Gilbert et al. (p. 1078 , published online 15 July; see the Perspective by Bhatia ) built an in vitro–culture system that resembles the physical characteristics in which muscle stem cells normally reside: a squishy elastic bed (rather than the hard slab of a plastic culture flask). Laminin tethered to hydrogels was used to generate substrates of varying elasticity. When cultured on these substrates, muscle stem cells remained undifferentiated and were able to support muscle regeneration when transplanted back into mice.

Bibliography

Gilbert, P. M., Havenstrite, K. L., Magnusson, K. E. G., Sacco, A., Leonardi, N. A., Kraft, P., Nguyen, N. K., Thrun, S., Lutolf, M. P., & Blau, H. M. (2010). Substrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in Culture. Science, 329(5995), 1078â1081.

Authors 10

P. M. Gilbert (first)
K. L. Havenstrite (additional)
K. E. G. Magnusson (additional)
A. Sacco (additional)
N. A. Leonardi (additional)
P. Kraft (additional)
N. K. Nguyen (additional)
S. Thrun (additional)
M. P. Lutolf (additional)
H. M. Blau (additional)

References 28 Referenced 1,360

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Dates

Type	When
Created	15 years, 1 month ago (July 15, 2010, 10:40 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 8:33 a.m.)
Indexed	1 week ago (Aug. 23, 2025, 1:06 a.m.)
Issued	15 years ago (Aug. 27, 2010)
Published	15 years ago (Aug. 27, 2010)
Published Print	15 years ago (Aug. 27, 2010)

Funders 0

None

BibTeX

@article{Gilbert_2010, title={Substrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in Culture}, volume={329}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1191035}, DOI={10.1126/science.1191035}, number={5995}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Gilbert, P. M. and Havenstrite, K. L. and Magnusson, K. E. G. and Sacco, A. and Leonardi, N. A. and Kraft, P. and Nguyen, N. K. and Thrun, S. and Lutolf, M. P. and Blau, H. M.}, year={2010}, month=aug, pages={1078–1081} }

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