DOI: 10.1126/science.1177662. Structural Insight into Nascent Polypeptide Chain

Structural Insight into Nascent Polypeptide Chain–Mediated Translational Stalling

10.1126/science.1177662

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Nascent Chains Revealed Detailed analysis of protein translation and translocation across membranes requires the identification and structural analysis of intermediates involved in these processes (see the Perspective by Kampmann and Blobel ). Seidelt et al. (p. 1412 , published online 29 October) report the visualization by cryo-electron microscopy of a nascent polypeptide chain in the tunnel of the ribosome at 5.8 angstroms. This resolution allows analysis of the conformation and distinct contacts of the nascent chain within the ribosomal tunnel, which suggests a mechanism by which translational stalling is induced by this peptide. Protein translocation across cellular membranes involves the Sec61 protein, a component of a protein-conducting channel. Whether Sec61 acts as a monomer or as an oligomer during protein translocation has been unclear. Becker et al. (p. 1369 , published online 29 October) describe active yeast and mammalian ribosome-Sec61 structures that show the Sec61 complex interacting with the ribosome and a nascent secretory protein signal sequence. The analysis unambiguously reveals that the active protein-conducting channel is a single Sec61 copy with its central pore serving as conduit for the nascent polypeptide.

Bibliography

Seidelt, B., Innis, C. A., Wilson, D. N., Gartmann, M., Armache, J.-P., Villa, E., Trabuco, L. G., Becker, T., Mielke, T., Schulten, K., Steitz, T. A., & Beckmann, R. (2009). Structural Insight into Nascent Polypeptide ChainâMediated Translational Stalling. Science, 326(5958), 1412â1415.

Authors 12

Birgit Seidelt (first)
C. Axel Innis (additional)
Daniel N. Wilson (additional)
Marco Gartmann (additional)
Jean-Paul Armache (additional)
Elizabeth Villa (additional)
Leonardo G. Trabuco (additional)
Thomas Becker (additional)
Thorsten Mielke (additional)
Klaus Schulten (additional)
Thomas A. Steitz (additional)
Roland Beckmann (additional)

References 26 Referenced 250

Dates

Type	When
Created	15 years, 9 months ago (Oct. 29, 2009, 10:19 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 3:30 a.m.)
Indexed	4 months ago (April 17, 2025, 12:28 p.m.)
Issued	15 years, 8 months ago (Dec. 4, 2009)
Published	15 years, 8 months ago (Dec. 4, 2009)
Published Print	15 years, 8 months ago (Dec. 4, 2009)

Funders 0

None

BibTeX

@article{Seidelt_2009, title={Structural Insight into Nascent Polypeptide Chain–Mediated Translational Stalling}, volume={326}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1177662}, DOI={10.1126/science.1177662}, number={5958}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Seidelt, Birgit and Innis, C. Axel and Wilson, Daniel N. and Gartmann, Marco and Armache, Jean-Paul and Villa, Elizabeth and Trabuco, Leonardo G. and Becker, Thomas and Mielke, Thorsten and Schulten, Klaus and Steitz, Thomas A. and Beckmann, Roland}, year={2009}, month=dec, pages={1412–1415} }

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  "abstract": "<jats:title>Nascent Chains Revealed</jats:title>\n          <jats:p>\n            Detailed analysis of protein translation and translocation across membranes requires the identification and structural analysis of intermediates involved in these processes (see the Perspective by\n            <jats:bold>\n              <jats:related-article xmlns:xlink=\"http://www.w3.org/1999/xlink\" ext-link-type=\"doi\" issue=\"5958\" page=\"1352\" related-article-type=\"in-this-issue\" vol=\"326\" xlink:href=\"10.1126/science.1183690\">Kampmann and Blobel</jats:related-article>\n            </jats:bold>\n            ).\n            <jats:bold>\n              Seidelt\n              <jats:italic>et al.</jats:italic>\n            </jats:bold>\n            (p.\n            <jats:related-article xmlns:xlink=\"http://www.w3.org/1999/xlink\" ext-link-type=\"doi\" page=\"1412\" related-article-type=\"in-this-issue\" vol=\"326\" xlink:href=\"10.1126/science.1177662\">1412</jats:related-article>\n            , published online 29 October) report the visualization by cryo-electron microscopy of a nascent polypeptide chain in the tunnel of the ribosome at 5.8 angstroms. This resolution allows analysis of the conformation and distinct contacts of the nascent chain within the ribosomal tunnel, which suggests a mechanism by which translational stalling is induced by this peptide. Protein translocation across cellular membranes involves the Sec61 protein, a component of a protein-conducting channel. Whether Sec61 acts as a monomer or as an oligomer during protein translocation has been unclear.\n            <jats:bold>\n              Becker\n              <jats:italic>et al.</jats:italic>\n            </jats:bold>\n            (p.\n            <jats:related-article xmlns:xlink=\"http://www.w3.org/1999/xlink\" ext-link-type=\"doi\" page=\"1369\" related-article-type=\"in-this-issue\" vol=\"326\" xlink:href=\"10.1126/science.1178535\">1369</jats:related-article>\n            , published online 29 October) describe active yeast and mammalian ribosome-Sec61 structures that show the Sec61 complex interacting with the ribosome and a nascent secretory protein signal sequence. The analysis unambiguously reveals that the active protein-conducting channel is a single Sec61 copy with its central pore serving as conduit for the nascent polypeptide.\n          </jats:p>",
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