DOI: 10.1126/science.1165216. De Novo Formation of a Subnuclear Body

10.1126/science.1165216

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The mammalian cell nucleus contains structurally stable functional compartments. We show here that one of them, the Cajal body (CB), can be formed de novo. Immobilization on chromatin of both CB structural components, such as coilin, and functional components of the CB, such as the SMN complex, spliceosomal small nuclear ribonucleoproteins (RNPs), small nucleolar RNPs, and small Cajal body–specific RNPs, is sufficient for the formation of a morphologically normal and apparently functional CB. Biogenesis of the CB does not follow a hierarchical assembly pathway and exhibits hallmarks of a self-organizing structure.

Bibliography

Kaiser, T. E., Intine, R. V., & Dundr, M. (2008). De Novo Formation of a Subnuclear Body. Science, 322(5908), 1713â1717.

Authors 3

Trish E. Kaiser (first)
Robert V. Intine (additional)
Miroslav Dundr (additional)

References 27 Referenced 221

10.1038/nrm1172
10.1016/j.tcb.2005.11.005
10.1016/j.cell.2007.01.028
10.1016/j.cell.2008.03.004
10.1007/s00418-007-0355-x
10.1242/jcs.00400
10.1083/jcb.200311121
Materials and methods are available as supporting material on Science online.
A. S. Belmontet al., Methods Cell Biol.58, 203 (1999). / Methods Cell Biol. (1999)
10.1083/jcb.200710058
10.1083/jcb.200104083
10.1101/gad.908401
10.1093/hmg/ddi168
10.1093/hmg/ddm192
10.1083/jcb.142.2.319
10.1016/j.ceb.2006.03.005
10.1091/mbc.e07-12-1259
10.1017/S135583820101442X
10.1016/S1097-2765(02)00484-7
10.1093/emboj/17.14.4114
10.1093/nar/gkl1172
10.1016/S0091-679X(08)85012-1
10.1083/jcb.200108110
10.1016/j.jtbi.2005.07.019
10.1242/jcs.114.24.4407
10.1038/nature05916
We thank A. Belmont E. Bertrand J. Gall M. Hastings P. Hemmerich T. Kiss I. Lemm R. Lührmann G. Matera T. Meier T. Misteli S. Sharma E. Soutoglou C. Verheggen and P. Young for reagents; S. Shevtsov for technical assistance; and T. Misteli for helpful suggestions and critical reading of the manuscript. This research was supported by Schweppe Career Development Award (the Schweppe Foundation) to M.D. and by startup funds from the Rosalind Franklin University of Medicine and Science to M.D.

Dates

Type	When
Created	16 years, 10 months ago (Oct. 23, 2008, 9:28 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 3:56 a.m.)
Indexed	2 weeks, 5 days ago (Aug. 6, 2025, 9:54 a.m.)
Issued	16 years, 8 months ago (Dec. 12, 2008)
Published	16 years, 8 months ago (Dec. 12, 2008)
Published Print	16 years, 8 months ago (Dec. 12, 2008)

Funders 0

None

BibTeX

@article{Kaiser_2008, title={De Novo Formation of a Subnuclear Body}, volume={322}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1165216}, DOI={10.1126/science.1165216}, number={5908}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Kaiser, Trish E. and Intine, Robert V. and Dundr, Miroslav}, year={2008}, month=dec, pages={1713–1717} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        6
      ]
    ],
    "date-time": "2025-08-06T13:54:41Z",
    "timestamp": 1754488481956
  },
  "reference-count": 27,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "5908",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2008,
        12,
        12
      ]
    ]
  },
  "abstract": "<jats:p>The mammalian cell nucleus contains structurally stable functional compartments. We show here that one of them, the Cajal body (CB), can be formed de novo. Immobilization on chromatin of both CB structural components, such as coilin, and functional components of the CB, such as the SMN complex, spliceosomal small nuclear ribonucleoproteins (RNPs), small nucleolar RNPs, and small Cajal body\u2013specific RNPs, is sufficient for the formation of a morphologically normal and apparently functional CB. Biogenesis of the CB does not follow a hierarchical assembly pathway and exhibits hallmarks of a self-organizing structure.</jats:p>",
  "DOI": "10.1126/science.1165216",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2008,
        10,
        24
      ]
    ],
    "date-time": "2008-10-24T01:28:16Z",
    "timestamp": 1224811696000
  },
  "page": "1713-1717",
  "source": "Crossref",
  "is-referenced-by-count": 221,
  "title": "De Novo Formation of a Subnuclear Body",
  "prefix": "10.1126",
  "volume": "322",
  "author": [
    {
      "given": "Trish E.",
      "family": "Kaiser",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Cell Biology, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA."
        },
        {
          "name": "Dr. William M. Scholl College of Podiatric Medicine at Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA."
        }
      ]
    },
    {
      "given": "Robert V.",
      "family": "Intine",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA."
        },
        {
          "name": "Dr. William M. Scholl College of Podiatric Medicine at Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA."
        }
      ]
    },
    {
      "given": "Miroslav",
      "family": "Dundr",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA."
        },
        {
          "name": "Dr. William M. Scholl College of Podiatric Medicine at Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA."
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_3_1_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nrm1172"
    },
    {
      "key": "e_1_3_1_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.tcb.2005.11.005"
    },
    {
      "key": "e_1_3_1_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.cell.2007.01.028"
    },
    {
      "key": "e_1_3_1_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.cell.2008.03.004"
    },
    {
      "key": "e_1_3_1_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s00418-007-0355-x"
    },
    {
      "key": "e_1_3_1_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/jcs.00400"
    },
    {
      "key": "e_1_3_1_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.200311121"
    },
    {
      "key": "e_1_3_1_9_2",
      "unstructured": "Materials and methods are available as supporting material on Science online."
    },
    {
      "key": "e_1_3_1_10_2",
      "first-page": "203",
      "volume": "58",
      "year": "1999",
      "unstructured": "A. S. Belmontet al., Methods Cell Biol.58, 203 (1999).",
      "journal-title": "Methods Cell Biol."
    },
    {
      "key": "e_1_3_1_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.200710058"
    },
    {
      "key": "e_1_3_1_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.200104083"
    },
    {
      "key": "e_1_3_1_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.908401"
    },
    {
      "key": "e_1_3_1_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/hmg/ddi168"
    },
    {
      "key": "e_1_3_1_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/hmg/ddm192"
    },
    {
      "key": "e_1_3_1_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.142.2.319"
    },
    {
      "key": "e_1_3_1_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.ceb.2006.03.005"
    },
    {
      "key": "e_1_3_1_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.e07-12-1259"
    },
    {
      "key": "e_1_3_1_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1017/S135583820101442X"
    },
    {
      "key": "e_1_3_1_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(02)00484-7"
    },
    {
      "key": "e_1_3_1_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/17.14.4114"
    },
    {
      "key": "e_1_3_1_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/gkl1172"
    },
    {
      "key": "e_1_3_1_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0091-679X(08)85012-1"
    },
    {
      "key": "e_1_3_1_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.200108110"
    },
    {
      "key": "e_1_3_1_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jtbi.2005.07.019"
    },
    {
      "key": "e_1_3_1_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/jcs.114.24.4407"
    },
    {
      "key": "e_1_3_1_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature05916"
    },
    {
      "key": "e_1_3_1_28_2",
      "unstructured": "We thank A. Belmont E. Bertrand J. Gall M. Hastings P. Hemmerich T. Kiss I. Lemm R. L\u00fchrmann G. Matera T. Meier T. Misteli S. Sharma E. Soutoglou C. Verheggen and P. Young for reagents; S. Shevtsov for technical assistance; and T. Misteli for helpful suggestions and critical reading of the manuscript. This research was supported by Schweppe Career Development Award (the Schweppe Foundation) to M.D. and by startup funds from the Rosalind Franklin University of Medicine and Science to M.D."
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.1165216",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        10
      ]
    ],
    "date-time": "2024-01-10T08:56:50Z",
    "timestamp": 1704877010000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.1165216"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2008,
        12,
        12
      ]
    ]
  },
  "references-count": 27,
  "journal-issue": {
    "issue": "5908",
    "published-print": {
      "date-parts": [
        [
          2008,
          12,
          12
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.1165216"
  ],
  "URL": "http://dx.doi.org/10.1126/science.1165216",
  "relation": {
    "has-review": [
      {
        "id-type": "doi",
        "id": "10.3410/f.1127049.584180",
        "asserted-by": "object"
      },
      {
        "id-type": "doi",
        "id": "10.3410/f.1127049.595006",
        "asserted-by": "object"
      }
    ]
  },
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        2008,
        12,
        12
      ]
    ]
  }
}