DOI: 10.1126/science.1161070. Helical Structures of ESCRT-III Are Disassembled by VPS4

Helical Structures of ESCRT-III Are Disassembled by VPS4

10.1126/science.1161070

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

During intracellular membrane trafficking and remodeling, protein complexes known as the ESCRTs (endosomal sorting complexes required for transport) interact with membranes and are required for budding processes directed away from the cytosol, including the budding of intralumenal vesicles to form multivesicular bodies; for the budding of some enveloped viruses; and for daughter cell scission in cytokinesis. We found that the ESCRT-III proteins CHMP2A and CHMP3 (charged multivesicular body proteins 2A and 3) could assemble in vitro into helical tubular structures that expose their membrane interaction sites on the outside of the tubule, whereas the AAA-type adenosine triphosphatase VPS4 could bind on the inside of the tubule and disassemble the tubes upon adenosine triphosphate hydrolysis. CHMP2A and CHMP3 copolymerized in solution, and their membrane targeting was cooperatively enhanced on planar lipid bilayers. Such helical CHMP structures could thus assemble within the neck of an inwardly budding vesicle, catalyzing late steps in budding under the control of VPS4.

Bibliography

Lata, S., Schoehn, G., Jain, A., Pires, R., Piehler, J., GoÌttlinger, H. G., & Weissenhorn, W. (2008). Helical Structures of ESCRT-III Are Disassembled by VPS4. Science, 321(5894), 1354â1357.

Authors 7

Suman Lata (first)
Guy Schoehn (additional)
Ankur Jain (additional)
Ricardo Pires (additional)
Jacob Piehler (additional)
Heinrich G. Gőttlinger (additional)
Winfried Weissenhorn (additional)

References 28 Referenced 316

10.1038/nrm2162
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10.1073/pnas.0603788103
S. Lataet al., J. Mol. Biol.378, 816 (2008). / J. Mol. Biol. (2008)
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10.1073/pnas.2133846100
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10.1038/374186a0
We thank X. Siebert (Institut de Biologie Structurale) for advice on UROX. This work was supported by Deutsche Forschungsgemeinschaft (SPP 1175) (W.W.) the Agence Nationale de la Recherche sur le SIDA (W.W.) Université Joseph Fourier (W.W.) the Agence Nationale de la Recherche (G.S.) the CNRS (G.S.) the NIH (grant AI29873 H.G.) and by postdoctoral fellowships from the European Molecular Biology Organization (S.L.) and the International Human Frontier Science Program Organization (S.L.). The EM map has been deposited at the European Bioinformatics Institute accession code EMD-1536.

Dates

Type	When
Created	17 years ago (Aug. 7, 2008, 9:35 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 3:23 a.m.)
Indexed	3 weeks, 6 days ago (Aug. 5, 2025, 8:34 a.m.)
Issued	16 years, 11 months ago (Sept. 5, 2008)
Published	16 years, 11 months ago (Sept. 5, 2008)
Published Print	16 years, 11 months ago (Sept. 5, 2008)

Funders 0

None

BibTeX

@article{Lata_2008, title={Helical Structures of ESCRT-III Are Disassembled by VPS4}, volume={321}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.1161070}, DOI={10.1126/science.1161070}, number={5894}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Lata, Suman and Schoehn, Guy and Jain, Ankur and Pires, Ricardo and Piehler, Jacob and Gőttlinger, Heinrich G. and Weissenhorn, Winfried}, year={2008}, month=sep, pages={1354–1357} }

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